Effect of propolis consumption on hepatotoxicity and brain damage in male rats exposed to chlorpyrifos

Newairy,; A, A; Abdou,; H, M

doi:10.5897/ajb12.2797

Cited by 36 publications

(21 citation statements)

References 46 publications

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“…These results are in agreement with who studied the effect of Propolis on CPF induced hepatic toxicity and reported serum reduction in thiobarbituric acid-reactive substances (TBARS) level and increased the activities of (SOD, CAT and GSH) [19]. Also with who studied the effect of Propolis on antioxidant status and reported that propolis caused reduction in the malondialdehyde (MDA) level and increased the activities of the antioxidant enzymes (SOD, GPX and CAT) [50,51].…”

Section: Journal Of Medical Toxicology and Clinical Forensic Medicinesupporting

confidence: 80%

The Propolis Effect on Chlorpyrifos Induced Thyroid Toxicity in Male Albino Rats

El-Sheikh¹,

Ibrahim²

2017

J Med Toxicol Clin Forens Med

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Chlorpyrifos (CPF) is a chlorinated organophosphate insecticide that is used widely in the world and Egypt. The aim of present work was to study the possible protective role of Propolis on thyroid gland of male rats after chronic exposure of chlorpyrifos. For this purpose, Fifty adult male albino rats were randomized into 5 groups: I, II (Control), III (received Propolis dissolved in 0.5 ml corn oil, 50 mg/kg b.w./day), IV (received CPF dissolved in 0.5 ml corn oil 6.75 mg/kg b.w./ day) and V (received CPF and Propolis at the same previously mentioned doses). Rats were orally treated by gavage for 12 weeks. We found that Chlorpyrifos induced damaging effects in thyroid tissue architecture, thickened collagen fibers between the follicles and decreased periodic acid schiff (PAS) reaction in the colloids. Immunohistochemically, there was weak thyroglobulin (TGB) expression. Besides, it significantly decreased the serum levels of Tri-iodothyronin (T3), Tetraiodothyronin (T4) and Thyroid stimulating hormone (TSH). It depressed reduced glutathione (GSH) content and antioxidant enzyme activities in thyroid tissues with respect to control groups. Furthermore, CPF increased thyroid oxidative stress as manifested by elevated malondialdehyde (MDA) levels. On the other hand, the concurrent administration of Propolis with CPF showed significant improvement in previous changes. Our findings showed that the concurrent oral administration of Propolis with CPF improved the CPF induced oxidative damaging effects in thyroid gland and the antioxidative defense in the rats.

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Section: Journal Of Medical Toxicology and Clinical Forensic Medicinesupporting

confidence: 80%

The Propolis Effect on Chlorpyrifos Induced Thyroid Toxicity in Male Albino Rats

El-Sheikh¹,

Ibrahim²

2017

J Med Toxicol Clin Forens Med

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“…Data cedera kepala di Rumah Sakit neuroprotektif. Oxygen-derived free radical mempunyai Dr. Wahidin Sudirohusodo Makasar tahun 2005 berjumlah implikasi terhadap patogenesis cedera otak setelah 861 kasus, tahun 2006 berjumlah 817 kasus dan tahun iskemia-reperfusi (11)(12)(13). CAPE dapat melindungi medula 2007 berjumlah 1078 kasus.…”

Section: Pendahuluanunclassified

“…antioksidan. Pemberian CAPE dan alpha-tocopherol dapat Disamping itu, berdasarkan hasil penelitian juga dapat menekan reperfusi iskemia akibat cerebral lipid dilihat bahwa semakin tinggi dosis ekstrak propolis yang peroxidation dan cedera otak (11)(12)(13). Selain itu, terapi diberikan akan semakin menurunkan ekspresi TLR4.…”

Section: Pendahuluanunclassified

Pengaruh Pemberian Ekstrak Propolis terhadap Ekspresi TLR4 dan Apoptosis pada Jaringan Otak Tikus Model Traumatic Brain Injury (TBI)

Prasetyo

Handono

Dalhar

2017

JKB

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Traumatic brain injury (TBI) merupakan cedera kepala yang sering terjadi pada populasi di seluruh dunia. Selama cedera kepala terjadi reaksi kaskade inflamasi yang dihubungkan dengan aktivasi TLR4. Propolis adalah produk lebah dengan kandungan flavonoid tinggi yang mempunyai efek antioksidan, anti inflamasi, proteksi DNA, neuroprotektan, dan immunomodulator. Kandungan propolis berupa Caffeic Acid Phenethyl Ester (CAPE) dapat menekan reperfusi iskemia, mencegah aktifasi TLR4, menghambat produksi sitokin proinflamasi dan menghambat produksi ROS pada level transkripsi melalui supresi aktivasi NFkB sehingga akan menghambat apoptosis. Penelitian ini bertujuan untuk menguji pengaruh pemberian ekstrak propolis dalam berbagai dosis pada ekspresi TLR4 dan apoptosis di jaringan otak tikus Rattus norvegicus model trauma kapitis. Metode yang digunakan dalam penelitian ini adalah imunohistokimia dan DNA terfragmentasi (TUNEL). Sampel dibagi menjadi 5 kelompok, yaitu: kelompok model trauma dan perlakuan propolis masing-masing dosis 50mg, 100mg, dan 200mg, kontrol positif dan kontrol negatif. Pada akhir penelitian, tikus dikorbankan dan dibuat preparat otak untuk menilai ekspresi TLR4 dan apoptosis. Berdasarkan hasil analisis statistik, didapatkan hubungan yang signifikan antara ekspresi TLR4 dan apoptosis sel otak tikus model traumatik dengan berbagai dosis propolis (p=0,000, r=-0,903, p=0,000, r=-0,907, Korelasi Pearson). Penelitian ini membuktikan bahwa propolis berpengaruh dalam penurunan ekspresi TLR4 dan apoptosis di sel otak tikus model trauma kapitis.

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“…The antioxidant activity of CAPE is due to the presence of two hydroxyl groups in its structure (57) . Also, Propolis contains acid derivatives such as benzoic-4-hydroxy benzoic which improves the digestive utilization of calcium, phosphorus and magnesium (58) . Therefore, the flavonoids of the Propolis can increase the activities of the antioxidant enzymes and reduce the levels of the ROS.…”

Section: (33)mentioning

confidence: 99%

Influence of Propolis, Nigella sativa on toxicity induced by Chlorpyrifos administration in adult male albino rats.

Ameen¹,

Keshta²,

AbdEl-Fatah³

2016

Biochemistry Letters

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Background: Chlorpyrifos (CPF) is one of the most widely used Organophosphates (OPs) insecticides in agriculture and public health. Natural products (Propolis and Nigella Sativa) are a promising source for the discovery of new pharmaceuticals. Aim: The present study was an attempt to evaluate the benefits of Propolis and Nigella Sativa alone and in combination against toxicity induced by Chlorpyrifos in on lung and heart tissues of adult male albino rats. Materials & Methods: 54 adult male albino rats were equally divided into 9 groups as following: Group I (negative control group); Group II: received the solvent. Group III (Propolis treated group): in which each rat received orally Propolis only rats (400mg/kg body weight) dissolved in corn oil. Group IV (Nigella Sativa oil treated group) each rat received orally Nigella Sativa oil (100 mg/kg) dissolved in corn oil. Group V: (Propolis and Nigella Sativa treated group). Group VI (CPF treated group): rats gavaged orally CPF at a daily dose of 6.75 mg/kg (1/20 of the oral LD50 of CPF (135mg/kg) dissolved in corn. Group 7 (CPF and Propolis treated group): rats gavaged orally Propolis before CPF administration. Group 8 (CPF and Nigella Sativa oil treated group): the rats gavaged orally Nigella Sativa before CPF. Group 9 (CPF, Nigella Sativa oil and Propolis treated group). Results: Chlorpyrifos induced a highly significant increase in lactate dehydrogenase, creatine kinase activities, elevation of Galctin-3 and troponin levels, and a significant reduction in acetylcholine esterase activity. All these alterations were confirmed histopathologically in lung and heart tissues, where Chlorpyrifos induced myocardial necrosis, muscular hyalinosis, bronchopneumonia and bronchitis. Meanwhile, Propolis and Nigella Sativa showed a protective action against Chlorpyrifos toxic effects especially when used in combination. Conclusion: It can be concluded that Propolis and Nigella Sativa had an ameliorating effect against this toxicity induced in adult albino rats.

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Effect of propolis consumption on hepatotoxicity and brain damage in male rats exposed to chlorpyrifos

Cited by 36 publications

References 46 publications

The Propolis Effect on Chlorpyrifos Induced Thyroid Toxicity in Male Albino Rats

The Propolis Effect on Chlorpyrifos Induced Thyroid Toxicity in Male Albino Rats

Pengaruh Pemberian Ekstrak Propolis terhadap Ekspresi TLR4 dan Apoptosis pada Jaringan Otak Tikus Model Traumatic Brain Injury (TBI)

Influence of Propolis, Nigella sativa on toxicity induced by Chlorpyrifos administration in adult male albino rats.

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