Effect of prolonged water stress on specialized secondary metabolites, peltate glandular trichomes, and pathway gene expression in Artemisia annua L.

Yadav, Radha; Sangwan, Rajender S.; Sabir, Farzana; Srivastava, Ankit; Sangwan, Neelam S.

doi:10.1016/j.plaphy.2013.10.023

Cited by 138 publications

(89 citation statements)

References 55 publications

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“…Stem, leaves and flowers presents T‐shaped filamentous trichomes and glandular biseriate trichomes (GT), (Figure ), whose average density was comparable to what was found by Kjær et al . (39.10 ± 2.50 GT/mm 2 ) and higher than those reported in studies performed either on populations grown in the field or under laboratory conditions (Table ) …”

Section: Resultssupporting

confidence: 84%

Optimisation of artemisinin and scopoletin extraction from Artemisia annua with a new modern pressurised cyclic solid–liquid (PCSL) extraction technique

Zarrelli

Pollio

Aceto

et al. 2019

Phytochemical Analysis

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Introduction Artemisia annua is a small herbaceous plant belonging to the Asteraceae family declared therapeutic by the World Health Organisation, in particular for its artemisinin content, an active ingredient at the base of most antimalarial treatments, used every year by over 300 million people. In the last years, owing to low artemisinin content, research of new ways to increase the yield of the plant matrix has led to the use of the total extract taking advantage from the synergic and stabilising effects of the other components. Objective In this work we evaluated and compared the content of artemisinin and scopoletin in extracts of A. annua collected in the Campania Region (southern Italy), by two different extraction processes. Methodology Artemisia annua plants were extracted by traditional maceration (TM) in hydroalcoholic solution as a mother tincture prepared according to the European Pharmacopeia and by pressurised cyclic solid–liquid (PCSL) extraction, a new generation method using the Naviglio extractor. Results The results showed that the PCSL extraction technique is more effective than traditional methods in extracting both phytochemicals, up to 15 times more, reducing the extraction times, without using solvents or having risks for the operators, the environment and the users of the extracts. Conclusion The Naviglio extractor provides extracts with an artemisinin and scopoletin content eight times higher than the daily therapeutic dose, which should be evaluated for its stability over time and biological properties for possible direct use for therapeutic purposes.

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Section: Resultssupporting

confidence: 84%

Optimisation of artemisinin and scopoletin extraction from Artemisia annua with a new modern pressurised cyclic solid–liquid (PCSL) extraction technique

Zarrelli

Pollio

Aceto

et al. 2019

Phytochemical Analysis

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“…Similarly Yadav et al . observed increase in proline content under water stressed plants. Total soluble protein content increased at earlier stages (EVS and LVS) under MS and SS, but it began to decrease at PFS under SS and continued to decrease until FS in both treatments.…”

Section: Discussionmentioning

confidence: 74%

Water deficit‐induced oxidative stress affects artemisinin content and expression of proline metabolic genes in Artemisia annua L.

Soni

Abdin

2017

FEBS Open Bio

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Water stress is one of the most critical abiotic stresses that restricts growth, development, and alters physiological and biochemical mechanisms of plant. The effects of long‐term water shortage‐induced oxidative stress on morphophysiological parameters, proline metabolic genes, and artemisinin content were studied in Artemisia annua L. under greenhouse conditions. Plant growth, biomass accumulation, relative water content, and chlorophyll content were reduced under drought. Leaf water potential ranged from −0.3248 MPa to −1.22 MPa in stress conditions. Increased levels of proline accumulation, protein concentration, and lipid peroxidation were detected in water‐stressed plants. Stage‐dependent increases in activity of antioxidants including superoxide dismutase, ascorbate peroxidase, glutathione reductase, monodehydroascorbate reductase, and dehydroascorbate reductase were observed. The expression of proline biosynthetic genes including pyrroline‐5‐carboxylase synthase1, 1‐pyrroline‐5‐carboxylase synthase2, and 1‐pyrroline‐5‐carboxylase reductase was induced, while the ornithine aminotransferase transcript showed a variable response and the expression of proline catabolic genes including proline dehydrogenase1, proline dehydrogenase1, and proline 5‐carboxylate dehydrogenase was reduced by water stress. Our results indicate that the glutamine pathway is predominant under drought stress in A. annua and a reduction of catabolic gene expression is adopted as a defense strategy in adverse conditions. Higher expression of biosynthetic genes and lower expression of catabolic genes at the preflowering stage confirmed the important role of proline in flower development. Artemisinin content decreased owing to water stress, but the slightly higher amounts were detected in leaves of severely stressed plants compared with moderately stressed plants. The artemisinin content of A. annua might be regulated by controlling irrigation regimes.

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“…Interestingly, it was observed that water deficit stress induces a decrease in the density and size of glandular trichomes, thereby negatively modulating artemisinin and its precursors (Yadav 2014). The cytosol, cooperated with chloroplasts, is responsible for artemisinin biosynthesis In plants, there are differential organelle-specific isoprenoid biosynthesis pathways, which mainly include the cytosolic mevalonate (MVA) pathway and the chloroplast methylerythritol 4-phosphate (MEP) pathway (Zeng et al 2008b).…”

Section: Glandular Trichomes Are Dominant Sites Storing Artemisininmentioning

confidence: 99%

An ecological implication of glandular trichome-sequestered artemisinin: as a sink of biotic/abiotic stress-triggered singlet oxygen

Jiang

Gao

Liao

et al. 2015

Preprint

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Artemisinin is accumulated in wormwood (Artemisia annua) with uncertain ecological implications. Here, we suggest that artemisinin is generated in response to biotic/abiotic stress, during which dihydroartemisinic acid, a direct artemisinin precursor, quenches singlet oxygen ( 1 O2), one kind of reactive oxygen species. Evidence supporting artemisinin as a sink of 1 O2 emerges from that volatile isoprenoids protect plants from biotic/abiotic stress; biotic/abiotic stress induces artemisinin biosynthesis; and stress signaling pathways are involved in the biosynthesis of volatile isoprenoids among plants as well as the biosynthesis of artemisinin in A. annua. In this review, we address the ecological implication of glandular trichome-sequestered artemisinin as a sink sink of biotic/abiotic stress-triggered 1 O2, and also summarize the cumulating data on the transcriptomic and metabolic profiling of stress-enhanced artemisinin production upon eliciting 1 O2 omission from chloroplasts and initiating retrograde 1 O2 signaling from chloroplasts to nuclei.

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Effect of prolonged water stress on specialized secondary metabolites, peltate glandular trichomes, and pathway gene expression in Artemisia annua L.

Cited by 138 publications

References 55 publications

Optimisation of artemisinin and scopoletin extraction from Artemisia annua with a new modern pressurised cyclic solid–liquid (PCSL) extraction technique

Optimisation of artemisinin and scopoletin extraction from Artemisia annua with a new modern pressurised cyclic solid–liquid (PCSL) extraction technique

Water deficit‐induced oxidative stress affects artemisinin content and expression of proline metabolic genes in Artemisia annua L.

An ecological implication of glandular trichome-sequestered artemisinin: as a sink of biotic/abiotic stress-triggered singlet oxygen

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