“…Structural modifications associated with freezing/thawing have been studied in several plant tissues through light microscopy (Buggenhout et al, 2006a;Khan & Vincent, 1996;Otero, Martino, Zaritzky, Solas, & Sanz, 2000;Sterling, 1968) and scanning electron microscopy (SEM) without (Delgado & Rubiolo, 2005;Sousa, Canet, Alvarez, & Tortosa, 2006) or with cryo-system (Cryo-SEM) (Alonso, Tortosa, Canet, & Rodriguez, 2005;Bomben, King, & Hayes, 1983; Martinez-Monzo, Martinez-Navarrete, Chiralt, & Fito, 1998;Tregunno & Goff, 1996). The impact of freezing on the microstructure of apples (Bomben et al, 1983), carrots (Buggenhout et al, 2006a), peaches (Otero et al, 2000), raspberries and blackberries (Sousa et al, 2006), blueberries and wild blackberries (Marti & Aguilera, 1991) and strawberries (Delgado & Rubiolo, 2005) have already been studied. The main drawback of microscopic techniques is sample preparation that is time consuming and the reduced field of view observed in a single image.…”