Effect of prematuration, meiosis activating sterol and enriched maturation medium on the nuclear maturation and competence to development of calf oocytes

Donnay, Isabelle; Faerge, I.; Grøndahl, C.; Verhaeghe, Benjamin; Sayoud, Hichem; Ponderato, Nunzia; Galli, Cesare; Lazzari, Giovanna

doi:10.1016/j.theriogenology.2003.12.019

Cited by 55 publications

(31 citation statements)

References 53 publications

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“…Similar patterns of in vitro development were observed in this study, but only with calf oocytes from 2-3 and 4-8 mm follicles, suggesting that the deficiencies described previously are restricted to these size categories. This may partially explain why calf oocytes from 2-8 mm follicles recently failed to improve their developmental competence after a 24 h prematuration step using inhibitors of meiosis [46].…”

Section: Discussionmentioning

confidence: 99%

The <i>In Vitro</i> Developmental Competence of Oocytes from Juvenile Calves is Related to Follicular Diameter

Kauffold¹,

Amer

Bergfeld³

et al. 2005

Journal of Reproduction and Development

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Abstract. This study investigated the relationship between follicle size (FS) and developmental competence of calf oocytes. Cumulus-oocyte-complexes (COCs) from follicles >8 (L-COCs; n=19), 4-8 (M-COCs; n=54), and 2-3 mm (S-COCs; n=155) were recovered from non-stimulated 1-4 months old dairy calves post mortem and ex vivo (laparoscopy), and in parallel from slaughtered adult cows from follicles of identical size categories [> 8 (n=91); 4-8 (n=138); 2-3 mm (n=193)]. Morphologically intact COCs were subjected to in vitro maturation, fertilization, and embryo culture. Cleavage rate (CR; 46 h post-insemination=p.i.), rate of morulae/blastocysts (M/Bl; day 7 p.i.), and blastocysts (Bl; day 9 p.i.) were recorded. FS had no effect on the CR in calves. However, calf L-COCs yielded the highest rates of M/Bl and Bl compared with the two other size categories (P<0.05). In contrast, calf S-and M-COCs gave similar rates of M/Bl, whereas the proportion of Bl was lowest for S-COCs (P<0.05). This was almost identical to findings in cows, except that the CR was highest for L-COCs and M/Bl yields were lowest for S-COCs (P<0.05). There were no differences between calf and cows with regard to CR for the respective FS categories. L-COCs from calves and cows yielded similar rates of M/Bl and Bl, whereas calf S-and M-COCs yielded lower rates of Bl than S-and M-COCs from cows and a lower rate of M/Bl when S-and M-COCs were analyzed as one group (P<0.05). Whereas the CR was similar in calves and cows, calf COCs yielded lower rates of M/Bl and Bl (P<0.05). In conclusion, the results show that the developmental competence of calf oocytes is higher in those derived from follicles larger than 8 mm, and thus are almost equally as competent as cow oocytes derived from follicles of identical size. This suggests that calf oocytes acquire developmental competence within the large follicle, potentially due to a process similar to prematuration of the oocyte in the adult cow. It is proposed that procedures that facilitate prematuration, such as "coasting" following a preceding superstimulation, might increase the developmental competence of calf oocytes.

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Section: Discussionmentioning

confidence: 99%

The <i>In Vitro</i> Developmental Competence of Oocytes from Juvenile Calves is Related to Follicular Diameter

Kauffold¹,

Amer

Bergfeld³

et al. 2005

Journal of Reproduction and Development

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“…COC with a compact and complete cumulus were selected and washed three times in HEPESbuffered tissue culture medium-199 (TCM-199) with gentamycin (50 mg/l). Groups of 50 COC were matured in vitro in 500 ml serum-free TCM-199 supplemented with ascorbic acid (75 mg/ml), L-cysteine (90 mg/ml), epidermal growth factor (EGF) (10 ng/ml), and fibroblast growth factor (FGF) from bovine pituitary (mainly FGF2, 2.2 ng/ml), b-glycine (720 mg/ml), glutamine (0.1 mg/ml), hCG (5 IU/ml), IGF1 (19 ng/ml), insulin (5 mg/ml), mercaptoethanol (0.1 mM), PMSG (10 IU/ml), pyruvate (110 mg/ml), selenium (5 ng/ml), and transferrin (5 mg/ml) (Donnay et al 2004). In vitro maturation (IVM) was performed for 24 h at 38.8 8C in humidified atmosphere consisting of 5% CO 2 and 95% air.…”

Section: Isolation and Culture Of Gcs And Cumulus-oocyte Complexesmentioning

confidence: 99%

Expression and effect of resistin on bovine and rat granulosa cell steroidogenesis and proliferation

Maillard¹,

Froment²,

Ramé³

et al. 2011

Reproduction

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Resistin, initially identified in adipose tissue and macrophages, was implicated in insulin resistance. Recently, its mRNA was found in hypothalamo-pituitary axis and rat testis, leading us to hypothesize that resistin may be expressed in ovary. In this study, we determined in rats and cows 1) the characterization of resistin in ovary by RT-PCR, immunoblotting, and immunohistochemistry and 2) the effects of recombinant resistin (10, 100, 333, and 667 ng/ml)GIGF1 (76 ng/ml) on steroidogenesis, proliferation, and signaling pathways of granulosa cells (GC) measured by enzyme immunoassay, [3 H]thymidine incorporation, and immunoblotting respectively. We observed that resistin mRNA and protein were present in several bovine and rat ovarian cells. Nevertheless, only bovine GC abundantly expressed resistin mRNA and protein. Resistin treatment decreased basal but not IGF1-induced progesterone (P!0.05; whatever the dose) and estradiol (P!0.005; for 10 and 333 ng/ml) production by bovine GC. In rats, resistin (10 ng/ml) increased basal and IGF1-induced progesterone secretion (P!0.0001), without effect on estradiol release. We found no effect of resistin on rat GC proliferation. Conversely, in cows, resistin increased basal proliferation (P!0.0001; for 100-667 ng/ml) and decreased IGF1-induced proliferation of GC (P!0.0001; for 10-333 ng/ml) associated with a decrease in cyclin D2 protein level (P!0.0001). Finally, resistin stimulated AKT and p38-MAPK phosphorylation in both species, ERK1/2-MAPK phosphorylation in rats and had the opposite effect on the AMPK pathway (P!0.05). In conclusion, our results show that resistin is expressed in rat and bovine ovaries. Furthermore, it can modulate GC functions in basal state or in response to IGF1 in vitro.

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“…COC with more than three layers of compact CC surrounding the oocyte were selected and washed several times in TCM199/HEPES medium supplemented with 50 mg/l of gentamycin. Groups of 50 COC were subjected to IVM in 500 ml of TCM199 serum-free medium supplemented with EGF (10 ng/ml), IGF-1 (19 ng/ml), fibroblast growth factor (2.2 ng/ml), hCG (5 UI/ml), pregnant mares serum gonadotrophin (10 UI/ml), insulin (5 mg/ml), transferrin (5 mg/ml), selenium (5 ng/ml), L-cystein (90 mg/ml), b-mercaptoethanol (0.1 mM), ascorbic acid (75 mg/ml), glycine (720 mg/ml), glutamine (0.1 mg/ml) and pyruvate (110 mg/ml) at 38.8 8C for 24 h in a humidified atmosphere containing 5% CO 2 as described earlier (Donnay et al 2004). LiCl 2 (final concentration of 20 mM if not specified) or BIO at 0.5-50 mM was added in culture medium just before IVM.…”

Section: Samples Collectionmentioning

confidence: 99%

Glycogen synthase kinase 3B in bovine oocytes and granulosa cells: possible involvement in meiosis during in vitro maturation

Uzbekova¹,

Salhab²,

Perreau³

et al. 2009

Reproduction

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Glycogen synthase kinase 3 (GSK3) regulates cellular metabolism and cell cycle via different signalling pathways. In response to insulin and growth factors GSK3 is serine-phosphorylated and inactivated. We analysed GSK3B expression and activation in bovine cumulus cells (CC) and oocytes at different meiotic stages in vitro in parallel with MAP kinases ERK (MAPK3/MAPK1) and p38 (MAPK14). GSK3B localised to cytoplasm in granulosa cells and in oocytes throughout folliculogenesis. In mature metaphase-II (MII) oocytes, GSK3B was concentrated to the region of midzone between the oocyte and the first polar body, as well as active phospho-Thr Aurora A kinase (AURKA). During in vitro maturation (IVM), in oocytes, phospho-Ser 9 -GSK3B level increased as well as phospho-MAPK3/MAPK1, while phospho-MAPK14 decreased. In CC, phospho-MAPK14 increased upon germinal vesicle breakdown (GVBD)/metaphase-I (MI) and then decreased during transition to MII. Administration of inhibitors of GSK3 activity (lithium chloride or 2 0 Z,3 0 E -6-bromoindirubin-3 0 -oxime) rapidly increased phospho-Ser 9 -GSK3B, and led to transient decrease of phospho-MAPK3/MAPK1 and to durable enhancing of phospho-MAPK14 in granulosa primary cell culture. GSK3 inhibitors during IVM diminished cumulus expansion and delayed meiotic progression. In cumulus, phospho-MAPK14 level was significantly higher in the presence of inhibitors, comparing with control, through the time of MI/MII transition. In oocytes, phospho-GSK3B was increased and phospho-MAPK3/MAPK1 was decreased before GVBD and oocytes were mainly arrested at MI. Therefore, GSK3B might regulate oocyte meiosis, notably MI/MII transition being the part of MAPK3/1 and MAPK14 pathways in oocytes and CC. GSK3B might be also involved in the local activation of AURKA that controls this transition.

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Effect of prematuration, meiosis activating sterol and enriched maturation medium on the nuclear maturation and competence to development of calf oocytes

Cited by 55 publications

References 53 publications

The <i>In Vitro</i> Developmental Competence of Oocytes from Juvenile Calves is Related to Follicular Diameter

The <i>In Vitro</i> Developmental Competence of Oocytes from Juvenile Calves is Related to Follicular Diameter

Expression and effect of resistin on bovine and rat granulosa cell steroidogenesis and proliferation

Glycogen synthase kinase 3B in bovine oocytes and granulosa cells: possible involvement in meiosis during in vitro maturation

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