The &lt;i&gt;In Vitro&lt;/i&gt; Developmental Competence of Oocytes from Juvenile Calves is Related to Follicular Diameter

Kauffold, Johannes; Amer, Hassan; Bergfeld, U.; Weber, Wolfgang; Sobiraj, A.

doi:10.1262/jrd.17002

Cited by 58 publications

(66 citation statements)

References 52 publications

(78 reference statements)

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“…The developmental and meiotic competence of oocytes isolated from different sized follicles has been intensively investigated in several species [8,[33][34][35][36]. It has been suggested that oocytes originating from large and medium follicles display an increased developmental potential compared with oocytes isolated from small follicles [8,37].…”

Section: Discussionmentioning

confidence: 99%

“…During each stage of follicle development, the somatic cells secrete several growth factors that have various effects on the maturation and development of the oocyte. The heterogeneity of follicular size has been implicated as the most important factor determining the developmental competence of oocytes in different stages of life [30][31][32].The developmental and meiotic competence of oocytes isolated from different sized follicles has been intensively investigated in several species [8,[33][34][35][36]. It has been suggested that oocytes originating from large and medium follicles display an increased developmental potential compared with oocytes isolated from small follicles [8,37].…”

mentioning

confidence: 99%

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Follicular Size is Associated with the Levels of Transcripts and Proteins of Selected Molecules Responsible for the Fertilization Ability of Oocytes of Puberal Gilts

Antosik

Kempisty²,

Bukowska

et al. 2009

Journal of Reproduction and Development

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Abstract. The maturation and developmental competence of the oocyte is acquired during folliculogenesis. It is still unclear whether follicle size is associated with the levels of transcript and protein encoding molecules contributing to the fertilization ability of the porcine oocyte. Follicles were dissected from porcine ovaries after slaughter and classified as small (< 3 mm), medium (3-5 mm) or large (>5 mm), aspirated cumulus-oocyte complexes were cultured in standard porcine IVM culture medium (TCM 199) for 44 h. In developmentally competent oocytes, assessed by determining the activity of glucose-6-phosphate dehydrogenase (G6PDH) using a brilliant cresyl blue (BCB) test, real-time quantitative PCR reaction methods, western-blot and confocal microscopy analysis were applied to determine the transcript levels of porcine zona pellucida glycoproteins pZP1, pZP2, pZP3, pZP3 alpha and integrins beta 1 and beta 2, as well as the levels of pZP3 and integrin beta 2 proteins. We observed significantly higher levels of pZP1, pZP3 and integrin beta1 and beta2 transcripts in oocytes collected from medium follicles as compared with small follicles (P<0.001). Moreover, we found an increased content of all investigated mRNAs in oocytes isolated from large follicles as compared with small follicles (P<0.001). Western-blot analysis demonstrated a higher level of pZP3 protein in oocytes isolated from large and medium follicles as compared with small follicles (P<0.001). Our results suggest that the levels of transcripts and proteins for selected molecules contributing to the fertilization ability of oocytes are associated with follicular size in puberal gilts. Key words: Follicular size, Integrins, Oocyte, Pig, ZPs glycoproteins (J. Reprod. Dev. 55: [588][589][590][591][592][593] 2009) ocyte quality has a significant influence on early embryonic development and survival, establishment and maintenance of pregnancy, fetal development and even the health of offspring in postnatal life [1][2][3][4][5]. The developmental competence of an oocyte may be affected by follicle size [6][7][8]. The growth of the oocyte within the follicle is accompanied by expression of a series of genes [9][10][11]. Some of these genes are involved in cell cycle control and meiotic progression, resulting in full meiotic competence of the oocyte [12,13]. Several other proteins are involved in cellular progress as characterized by complete developmental competence of the gamete prior to fertilization [14,15]. During the growth phase, several messenger RNA (mRNA) and their respective proteins are accumulated and stored long-term to be used after fertilization and subsequent proper embryo development [1,16]. The abilities of fertilization and embryo development are acquired by an oocyte only after a long period of growth and development. Specific follicular growth and somatic follicular cell-oocyte interactions lead to the acquisition of high developmental potential for the oocyte.The porcine zona pellucida is composed of four major glycoproteins: ZP1...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Follicular Size is Associated with the Levels of Transcripts and Proteins of Selected Molecules Responsible for the Fertilization Ability of Oocytes of Puberal Gilts

Antosik

Kempisty²,

Bukowska

et al. 2009

Journal of Reproduction and Development

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“…However, currently the average rates of viable blastocysts produced from prepubertal donor oocytes are lower than the use of adult oocytes (10-15 vs w30%; Oropeza et al 2004, Zaraza et al 2010. Incomplete or deficient cytoplasmic maturation, altered protein synthesis, reduced oocyte size, and impaired metabolism in prepubertal oocytes have been proposed as possible mechanisms for the reduced developmental potential (Lonergan et al 1994, Steeves et al 1999, De Paz et al 2001, Kauffold et al 2005.…”

Section: Introductionmentioning

confidence: 99%

DNA methylation and mRNA expression profiles in bovine oocytes derived from prepubertal and adult donors

Diederich¹,

Hansmann²,

Heinzmann³

et al. 2012

Reproduction

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The developmental capacity of oocytes from prepubertal cattle is reduced compared with their adult counterparts, and epigenetic mechanisms are thought to be involved herein. Here, we analyzed DNA methylation in three developmentally important, nonimprinted genes (SLC2A1, PRDX1, ZAR1) and two satellite sequences, i.e. 'bovine testis satellite I' (BTS) and 'Bos taurus alpha satellite I' (BTaS). In parallel, mRNA expression of the genes was determined by quantitative real-time PCR. Oocytes were retrieved from prepubertal calves and adult cows twice per week over a 3-week period by ultrasound-guided follicular aspiration after treatment with FSH and/or IGF1. Both immature and in vitro matured prepubertal and adult oocytes showed a distinct hypomethylation profile of the three genes without differences between the two types of donors. The methylation status of the BTS sequence changed according to the age and treatment while the methylation status of BTaS sequence remained largely unchanged across the different age and treatment groups. Relative transcript abundance of the selected genes was significantly different in immature and in vitro matured oocytes; only minor changes related to origin and treatment were observed. In conclusion, methylation levels of the investigated satellite sequences were high (O50%) in all groups and showed significant variation depending on the age, treatment, or in vitro maturation. To what extent this is involved in the acquisition of developmental competence of bovine oocytes needs further study.

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“…However, in a few studies, blastocysts have been produced from oocytes of young calves without prior superstimulation [15][16][17].…”

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confidence: 99%

Offspring from Non-stimulated Calves at an Age Younger than Two Months: A Preliminary Report

Kauffold¹,

Amer

Bergfeld³

et al. 2005

Journal of Reproduction and Development

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Abstract. This study investigated the viability of embryos from non-stimulated 2-3-month-old calves generated in vitro using oocytes from follicles of defined size in terms of their ability to produce fullterm pregnancies. Ablation of follicles ≥4 mm was used to induce the emergence of a new follicular wave, and calves (n=3) were laparoscopically punctured three times at 7-day-intervals to recover cumulus-oocyte-complexes (COCs) from follicles >8 (group A) and between 4-8 mm (group B). Calves were aged 49, 56, and 80 days, respectively, at first recovery. Morphologically intact COCs were subjected to in vitro maturation, fertilization, and embryo culture, and compact morulae/ blastocysts were transferred on day 7 post-insemination to synchronized virgin heifers. Blood typing was used for maternity analysis. A total of 29 COCs were recovered, 21 cultured, yielding 11 cleaved embryos (52.4%) and 6 compact morulae/blastocysts (28.6%). No differences were observed between groups. Transfer of the 6 embryos to 5 recipients resulted in three pregnancies (one from group A and two from group B). Two normal male offspring (both from group B), with birth weights of 44 and 51 kg, were born, and two donor calves, aged 56 and 59 days, were identified as the dams. In conclusion, the results demonstrate that embryos generated in vitro from oocytes from non-stimulated calves at an age younger than two months are viable in terms of their ability to produce full-term pregnancies, and suggest that the viability of calf embryos is not related to follicle size.

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The <i>In Vitro</i> Developmental Competence of Oocytes from Juvenile Calves is Related to Follicular Diameter

Cited by 58 publications

References 52 publications

Follicular Size is Associated with the Levels of Transcripts and Proteins of Selected Molecules Responsible for the Fertilization Ability of Oocytes of Puberal Gilts

Follicular Size is Associated with the Levels of Transcripts and Proteins of Selected Molecules Responsible for the Fertilization Ability of Oocytes of Puberal Gilts

DNA methylation and mRNA expression profiles in bovine oocytes derived from prepubertal and adult donors

Offspring from Non-stimulated Calves at an Age Younger than Two Months: A Preliminary Report

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