Effect of preharvest anti-fungal compounds on Aspergillus steynii and A. carbonarius under fluctuating and extreme environmental conditions

García-Cela, Esther; Gil-Serna, Jéssica; Marı́n, Sonia; Acevedo, H. Carhuancho; Patiño, Belén; Ramos, Antonio J.

doi:10.1016/j.ijfoodmicro.2012.08.001

Cited by 10 publications

(6 citation statements)

References 41 publications

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“…Nonetheless, the possible degradation to other compounds was not evaluated, as it occurs on maize, where a natural transformation from transzearalenone (ZEN) to cis-ZEN after 24 h of UV-A irradiation (3.2 mW/cm2) can occur (Brezina et al 2013). Similarly, incubation of A. carbonarius under two simulated climatic conditions characterized by alternating temperature cycles (10/25 ºC and 15/35 ºC) with photoperiod (14/10 h lightness/darkness), and two moisture levels (40 % and 25 %) for 21 d, showed that the extreme conditions tested caused a significant OTA reduction contamination (García-Cela et al 2012). Therefore, Aspergillus presence on crops under future climate conditions proposed by Southern Europe (EC 2007), could not be compromised; although the mycotoxin risk in vivo derived from predicted climatic conditions characterized by high temperatures, drier conditions, and increased UV could be reduced.…”

Section: Discussionmentioning

confidence: 84%

Effect of ultraviolet radiation A and B on growth and mycotoxin production by Aspergillus carbonarius and Aspergillus parasiticus in grape and pistachio media

et al. 2015

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The effects of two exposure times per day (6 and 16 h) of UV-A or UV-B radiation, combined with dark and dark plus light incubation periods during 7-21 d on fungal growth and mycotoxins production of Aspergillus species were studied. Aspergillus carbonarius and Aspergillus parasiticus were inoculated on grape and pistachio media under diurnal and nocturnal temperatures choosing light photoperiod according to harvest conditions of these crops in Spain. Ultraviolet irradiation had a significant effect on A. carbonarius and A. parasiticus colony size (diameter, biomass dry weight, and colony density) and mycotoxin accumulation, although intraspecies differences were observed. Inhibition of A. carbonarius fungal growth decreased when exposure time was reduced from 16 h to 6 h, but this was not always true for ochratoxin A (OTA) production. OTA reduction was higher under UV-A than UV-B radiation and the reduction increased along time conversely to the aflatoxins (AFs). Aflatoxin B1 (AFB1) was the main toxin produced by A. parasiticus except in the UV-B light irradiated colonies which showed a higher percentage of AFG than AFB. Morphological changes were observed in colonies grown under UV-B light.

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Section: Discussionmentioning

confidence: 84%

Effect of ultraviolet radiation A and B on growth and mycotoxin production by Aspergillus carbonarius and Aspergillus parasiticus in grape and pistachio media

et al. 2015

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“…No previous report has examined the impact that environmental conditions have on growth of A. steynii and A. ochraceus isolated from Spanish maize and on OTA production in maize kernels. As far as we know, only a study on wheat has been published [28] using ochratoxigenic isolates obtained from other hosts (barley and grapes). In that paper [28], very high OTA levels were found in wheat cultures of A. steynii incubated at 25/35 º C cycle, which surpass 1 mg/kg.…”

Section: Resultsmentioning

confidence: 99%

“…As far as we know, only a study on wheat has been published [28] using ochratoxigenic isolates obtained from other hosts (barley and grapes). In that paper [28], very high OTA levels were found in wheat cultures of A. steynii incubated at 25/35 º C cycle, which surpass 1 mg/kg. The assays were performed by inoculation of seeds on the dish center with 5 μL of a spore suspension of fungi of 10 6 spores/mL.…”

Section: Resultsmentioning

confidence: 99%

Environmental Temperature and Relative Humidity, two Key Factors in Maize Technology Affecting Ochratoxin a Production and Growth of Ochratoxigenic Species

Mateo¹,

Moliner²,

Gómez³

et al. 2018

ijfe

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Ochratoxin A (OTA) is a secondary fungal metabolite produced naturally by filamentous fungi of the Aspergillus and Penicillium genera. The aims of this study were to identify the most relevant OTA producing species in maize in Spain by PCR and to study the effect of environmental conditions (a w , temperature) on the development of these fungi and on OTA production in maize. Eighteen and 20 corn samples harvested in 2014 and 2015, respectively, from conventional farms and 31 and 34 corn samples harvested in 2014 and 2015, respectively, from organic farms located in various Spanish areas were tested for presence of ochratoxin-producing fungi. The most important OTA-producing Aspergillus spp. were isolated and identified by species-specific PCR protocols. The growth rate of the main ochratoxigenic species (A. steynii and A. ochraceus) under different environmental conditions was registered and OTA level in cultures was determined by solvent extraction, immunoaffinity column clean-up and LC-fluorescence detection. The results revealed that A. steynii and A. ochraceus are good indicators of OTA risk in maize. Temperature, a w , and isolate influenced OTA production. The highest OTA levels were found in maize cultures of A. steynii at 0.98 a w and 30 ºC. 

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“…In vitro seed germination and multiplication of Calophyllum brasiliense a)Fungicide added to culture medium Cercobin ® is a systemic fungicide. Its active compound is thiophanate methyl which combats fungal contamination due to the recognized property of its group, benzimidazoles, of preventing tubulin polymerization during metaphase, thereby causing inhibition of mitotic divisions and cell proliferation (García-Cela et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

In vitro seed germination and multiplication of Calophyllum brasiliense

2016

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Calophyllum brasiliense is a tree species with limited natural reproduction. In vitro germination may be an alternative for obtaining high-quality seedlings. Seeds were maintained in water before surface disinfestation and compared with control seeds (i.e. not immersed), without differences between treatments. HgCl2 used during surface-disinfestation reduced contamination rates of cultures. Fungal contamination was reduced with fungicide added to culture medium (23 to 6.4%), although bacterial contamination increased (24 to 36%). In another experiment, seeds were immersed in plant preservative mixture (PPM™) prior to surface disinfestation. By combining immersion for 48 h and 2 mL L-1 in culture medium, contamination was only 6%. Seeds immersion in GA3 prior to surface disinfestation reduced root formation as concentration increased. Germination rate and GSI were reduced, respectively, from 72% and 0.129 (24 h) to 60% and 0.092 (48 h) according to exposure time to GA3. After 90 days in multiplication medium containing benzylaminopurine, average number of shoots per nodal segment was 3.4. In conclusion, in vitro germination of C. brasiliense seeds is feasible in sucrose-free WPM medium and reaches a high contamination-free rate (up to 93.3%).

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Effect of preharvest anti-fungal compounds on Aspergillus steynii and A. carbonarius under fluctuating and extreme environmental conditions

Cited by 10 publications

References 41 publications

Effect of ultraviolet radiation A and B on growth and mycotoxin production by Aspergillus carbonarius and Aspergillus parasiticus in grape and pistachio media

Effect of ultraviolet radiation A and B on growth and mycotoxin production by Aspergillus carbonarius and Aspergillus parasiticus in grape and pistachio media

Environmental Temperature and Relative Humidity, two Key Factors in Maize Technology Affecting Ochratoxin a Production and Growth of Ochratoxigenic Species

In vitro seed germination and multiplication of Calophyllum brasiliense

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