Effect of Precursors and Organic Compounds on Alkaloid Production in Transformed Root Cultures of<i>Catharanthus roseus</i>. var.<i>nirmal</i>.

Gaviraj, E.N.; Veeresham, Ciddi

doi:10.1080/13880200600748929

Cited by 10 publications

(4 citation statements)

References 22 publications

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“…For the increase of TIA content in CHR, there were several studies using terpenoid precursor such as loganin that could enhance the catharanthine by 26%, ajmalicine by 84%, lochnericine by 119% and tabersonine by 225% in the induced samples (23). A study, it was reported that with loganin feeding, ajmalicine content (3.27 mg L -1 ) was improved by 2.3-fold and there was 1.8-fold increase in the yield of serpentine (2.51 mg L -1 ) when compared with control cultures (1.42 and 1.39 mg L -1 respectively) (24). Our study was firstly using PHEL and TYR for elevating VBL and VCR in CHR.…”

Section: Effects Of L-tyrosinementioning

confidence: 96%

In vitro growth and content of vincristine and vinblastine of Catharanthus roseus L. hairy roots in response to precursors and elicitors

Cao

Bui

et al. 2022

Plant Sci. Today

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Catharanthus roseus L. is a medicinal plant that produces numerous indole terpenoid alkaloids, including vincristine and vinblastine, which are used for cancer treatment. The effect of specified precursors (L-phenylalanine, L-tyrosine) and elicitors (chitosan, methyl jasmonate) on C. roseus hairy roots (CHR) growth has been examined in order to increase the content of vincristine and vinblastine. Our results showed that CHR generated by an Agrobacterium rhizogenes strain isolated in Vietnam was capable of producing both vincristine and vinblastine when subjected to precursors, but only vinblastine when exposed to elicitors. However, both precursors and elicitors were evaluated to have an effect on increasing the accumulation of TIAs in CHR. In particular, the use of elicitors required more time to find the appropriate induction conditions, while the use of precursors gave outstanding efficiency in the treatment with 1 µM phenylalanine. The greatest yields of vincristine (51.99 µg g-1 DW) and vinblastine (699.92 µg g-1 DW) were obtained in the 7th week (with 0.306 g DW biomass). This result is the first time we might boost the levels of vincristine and vinblastine in our CHR clone generated by the Vietnam strain of A. rhizogenes.

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Section: Effects Of L-tyrosinementioning

confidence: 96%

In vitro growth and content of vincristine and vinblastine of Catharanthus roseus L. hairy roots in response to precursors and elicitors

Cao

Bui

et al. 2022

Plant Sci. Today

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“…Apart from their involvement in transcriptional gene regulation, the JA and allied compounds also act as transducers of elicitor signals for the production and/or promotion and accumulation of various groups of SMs including alkaloids [44,45], Coumarines and furocoumarines [46], antraquinones and saponins [47], etc. in HRs and cell suspension systems.…”

Section: Transcription Factors Involved In Elicitation and Activationmentioning

confidence: 99%

Elicitor-Induced Cellular and Molecular Events Are Responsible for Productivity Enhancement in Hairy Root Cultures: An Insight Study

Goel

Mehrotra

Kukreja

2011

Appl Biochem Biotechnol

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A wide range of external stress stimuli triggers a plant cell to undergo a complex network of reactions that ultimately lead to the synthesis and accumulation of secondary metabolites. These secondary metabolites help the plant to survive under stress challenge. The potential of biotic and abiotic elicitors for the induction and enhancement of secondary metabolite production in various culture systems including hairy root (HR) cultures is well-known. The elicitor-induced defense responses involves signal perception of elicitor by a cell surface receptor followed by its transduction involving some major cellular and molecular events including activation of major secondary message signaling pathways. This result in induction of gene expressions escorting to the synthesis of various proteins mainly associated with plant defense responses and secondary metabolite synthesis and accumulation. The review discusses the elicitor-induced various cellular and molecular events and correlates them with enhanced secondary metabolite synthesis in HR systems. Further, this review also concludes that combining elicitation with in-silico approaches enhances the usefulness of this practice in better understanding and identifying the rate-limiting steps of biosynthetic pathways existing in HRs which in turn can contribute towards better productivity by utilizing metabolic engineering aspects.

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“…The amino acids added to cell suspension culture enhanced the production of tropane and indole alkaloids[11] as well as supplementation of precursors, organic compounds and multiple feedings of loganin increased the biosynthesis followed by production of indole alkaloids in transformed roots of Catharanthus roseus . [12]…”

Section: Introductionmentioning

confidence: 99%

In vitro production of alkaloids: Factors, approaches, challenges and prospects

Ahmad¹,

Garg²,

Tamboli³

et al. 2013

Phcog Rev

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The wide diversity of plant secondary metabolites is largely used for the production of various pharmaceutical compounds. In vitro cell tissue or organ culture has been employed as a possible alternative to produce such industrial compounds. Tissue culture techniques provide continuous, reliable, and renewable source of valuable plant pharmaceuticals and might be used for the large-scale culture of the plant cells from which these secondary metabolites can be extracted. Alkaloids are one of the most important secondary metabolites known to play a vital role in various pharmaceutical applications leading to an increased commercial importance in recent years. The tissue culture techniques may be utilized to improve their production of alkaloids via somaclonal variations and genetic transformations. The focus of this review is toward the application of different tissue culture methods/techniques employed for the in vitro production of alkaloids with a systematic approach to improve their production.

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Effect of Precursors and Organic Compounds on Alkaloid Production in Transformed Root Cultures ofCatharanthus roseus. var.nirmal.

Cited by 10 publications

References 22 publications

In vitro growth and content of vincristine and vinblastine of Catharanthus roseus L. hairy roots in response to precursors and elicitors

In vitro growth and content of vincristine and vinblastine of Catharanthus roseus L. hairy roots in response to precursors and elicitors

Elicitor-Induced Cellular and Molecular Events Are Responsible for Productivity Enhancement in Hairy Root Cultures: An Insight Study

In vitro production of alkaloids: Factors, approaches, challenges and prospects

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