Effect of Potentilla fulgens on lipid peroxidation and antioxidant status in alloxan-induced diabetic mice

Saio, Valrielyn; Syiem, Donkupar; Sharma, Ramesh

doi:10.4103/0976-0105.103816

Cited by 10 publications

(5 citation statements)

References 40 publications

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“…Hydrogen peroxide (H 2 O 2 ), generated by the dismutation reaction of SODs, is neutralized by CAT and hence, cooperative activity of these enzymes is required to maintain the redox homeostasis. In our study, the decreased activity of CAT in diabetic mice is consistent with some earlier reports . The decreased activity of investigated enzymes might be due to the inactivation caused by free radicals and/or by nonenzymatic glycation due to the persistent hyperglycemia.…”

Section: Discussionsupporting

confidence: 93%

“…Other prominent biomarkers are protein carbonyls that form due to the direct oxidation of certain amino acid residues by ROS or by their interaction with aldehyde containing lipid peroxidation products . The elevation of these biomarkers under the hyperglycemic condition is reported from several studies on diabetic patients as well as animal models . A significant increase of MDA along with protein carbonyl levels was also noticed in STZ‐induced diabetic mice in our study.…”

Section: Discussionsupporting

confidence: 78%

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Chlorophyllin supplementation modulates hyperglycemia‐induced oxidative stress and apoptosis in liver of streptozotocin‐administered mice

et al. 2018

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Chlorophyllin is a water-soluble mixture of sodium-copper salts of chlorophyll with antioxidant and antimutagen properties. In this study, an attempt has been made to evaluate the effect of chlorophyllin on hyperglycemia-induced oxidative stress and apoptosis in liver of streptozotocin (STZ)-administered mice. In STZ-induced diabetes, two causative factors for pancreatic β-cell deaths are DNA alkylation and profound reactive oxygen species (ROS) generation. In this study, chlorophyllin treatment was found to be able to modulate oxidative stress and apoptosis in liver of diabetic mice. Diabetic mice exhibited a significant reduction of ROS, malondialdehyde (MDA), and protein carbonyl levels upon treatment with the chlorophyllin. However, antioxidant enzymes, such as copperzinc superoxide dismutase (CuZnSOD), manganese superoxide dismutase (MnSOD), and catalase (CAT) showed enhanced activity as well as expression in chlorophyllin-administered diabetic mice. The hepatoprotective effect of chlorophyllin was confirmed from the decreased activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). The histological and ultrastructural studies revealed the ability of chlorophyllin to restore morphological and cellular alterations as observed in STZ-induced diabetic mice. The effect of chlorophyllin on apoptosis showed the downregulation of cysteine-dependent aspartatespecific protease (caspase) 3 and caspase 9, whereas upregulation of B-cell lymphoma-2 (Bcl-2) protein, and the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) assay demonstrated a few apoptotic cells. In conclusion, it can be stated that chlorophyllin treatment can exert hepatoprotective effect via modulating hyperglycemia-induced oxidative stress and apoptosis in STZ-administered diabetic mice. © 2018 BioFactors, 44(5): [418][419][420][421][422][423][424][425][426][427][428][429][430] 2018

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 78%

Chlorophyllin supplementation modulates hyperglycemia‐induced oxidative stress and apoptosis in liver of streptozotocin‐administered mice

et al. 2018

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“…MDA level in alloxan-induced diabetic mice was found to be higher than that in non-diabetic mice. This could be due to the establishment of chronic hyperglycemia and effect of alloxan [ 41 ]. Treatment of diabetic mice with Mo -LPI (500 mg/kg·bw; i.p.)…”

Section: Discussionmentioning

confidence: 99%

A Protein Isolate from Moringa oleifera Leaves Has Hypoglycemic and Antioxidant Effects in Alloxan-Induced Diabetic Mice

et al. 2017

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Moringa oleifera has been used in traditional medicine to treat diabetes. However, few studies have been conducted to relate its antidiabetic properties to proteins. In this study, a leaf protein isolate was obtained from M. oleifera leaves, named Mo-LPI, and the hypoglycemic and antioxidant effects on alloxan-induced diabetic mice were assessed. Mo-LPI was obtained by aqueous extraction, ammonium sulphate precipitation and dialysis. The electrophoresis profile and proteolytic hydrolysis confirmed its protein nature. Mo-LPI showed hemagglutinating activity, cross-reaction with anti-insulin antibodies and precipitation after zinc addition. Single-dose intraperitoneal (i.p.) administration of Mo-LPI (500 mg/kg·bw) reduced the blood glucose level (reductions of 34.3%, 60.9% and 66.4% after 1, 3 and 5 h, respectively). The effect of Mo-LPI was also evidenced in the repeated dose test with a 56.2% reduction in the blood glucose level on the 7th day after i.p. administration. Mo-LPI did not stimulate insulin secretion in diabetic mice. Mo-LPI was also effective in reducing the oxidative stress in diabetic mice by a decrease in malondialdehyde level and increase in catalase activity. Mo-LPI (2500 mg/kg·bw) did not cause acute toxicity to mice. Mo-LPI is a promising alternative or complementary agent to treat diabetes.

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“…Leaf, flower, and stem extracts of P. fruticosa, in the experiment using egg yolk, have been declared to demonstrate strong peroxidation inhibition (Yu et al 2016). In alloxaninduced diabetic rats P. fulgens at 250 mg kg -1 bw dose caused a reduction (75%) in TBARS levels in liver tissue (Saio et al 2012). Strong antioxidant properties of Potentilla species are also prominent with their lipid peroxidation inhibitory activities, and in our study, strong inhibition of P. recta on lipid peroxidation was reported by us for the first time.…”

Section: Albicansmentioning

confidence: 99%

Evaluation of Antioxidant and Antimicrobial Activities of Potentilla recta L.

Karatoprak

ÖKDEM

İlgün

et al. 2022

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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The objective of this study was to determine the effectiveness and the antioxidant activity of Potentilla recta L. (Rosaceae), which is traditionally used in the treatment of many diseases, against pathogenic microorganisms in the skin. The antioxidant activity was determined using 1,1-Diphenyl-2-picrylhydrazyl (DPPH • ) and 2,2-Azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS •+ ) radical scavenging activities, inhibition of β-carotene bleaching, protection of 2-deoxyribose and bovine brain-derived phospholipids against hydroxyl radical-mediated degradation assay, at different concentrations ranging from 0.001 to 2 mg mL -1 . Antimicrobial activity was evaluated against Staphylococcus aureus (ATCC 29213), Staphylococcus epidermidis (ATCC 3699), Pseudomonas aeruginosa (ATCC 27853) and Candida albicans (ATCC 90028) by microdilution method. Besides, viability enhancing effects on murine fibroblast cells (L929) were determined by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide) method. The key parameters for the extract included the following: DPPH • IC50: 0.19 mg mL -1 , TEAC: 0.76 mmol L -1 Trolox, reduction: 0.87 mmol g -1 AsscE, and protection of lipid peroxidation IC50: 0.07 mg mL -1 . A strong effect on S. epidermidis was observed with 79% inhibition at a concentration of 125 mg mL -1 but did not show toxicity to L929 cells below 250 mg mL -1 concentration. The present findings highlight the need for research of plants for traditional medicinal uses and the importance of scientific evaluations.

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Effect of Potentilla fulgens on lipid peroxidation and antioxidant status in alloxan-induced diabetic mice

Cited by 10 publications

References 40 publications

Chlorophyllin supplementation modulates hyperglycemia‐induced oxidative stress and apoptosis in liver of streptozotocin‐administered mice

Chlorophyllin supplementation modulates hyperglycemia‐induced oxidative stress and apoptosis in liver of streptozotocin‐administered mice

A Protein Isolate from Moringa oleifera Leaves Has Hypoglycemic and Antioxidant Effects in Alloxan-Induced Diabetic Mice

Evaluation of Antioxidant and Antimicrobial Activities of Potentilla recta L.

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