Effect of post-pretreatment washing on saccharification and co-fermentation from bagasse pretreated with biocompatible cholinium ionic liquid

Abstract

a b s t r a c tCholine acetate (ChOAc), a cholinium ionic liquid (IL), was used for biomass pretreatment; thereafter, saccharification of the pretreated biomass and glucose/xylose co-fermentation were conducted with different number of post-pretreatment washes (1-5 and 10 washes). Besides saccharification and cofermentation data, the inhibitory effect of ChOAc on the biocatalyst was compared with that of 1-ethyl-3-methylimidazolium acetate (EmimOAc). ChOAc showed less inhibitory effect on cellulase and yeast activity compared with EmimOAc. The cellulose and hemicellulose saccharification percentages of the IL-pretreated bagasse were approximately 90% and 60%, respectively, irrespective of ChOAc and EmimOAc with sufficient post-pretreatment washes. The cellulose and hemicellulose saccharification percentages after 5 washes were 82% and 59%, respectively, in the case of ChOAc and 51% and 13%, respectively, in the case of EmimOAc. The overall ethanol yields on the original bagasse basis after saccharification and co-fermentation of the IL-pretreated bagasse when washed 5 times were 54% and 22% in the case of ChOAc and EmimOAc, respectively. ChOAc-pretreated bagasse could be saccharified and co-fermented with fewer washes than EmimOAc-pretreated bagasse.

Evaluation of several ionic liquids for in situ hydrolysis of empty fruit bunches by locally-produced cellulase

“…Aside from the enzymes’ compatibility, the high cost of ILs and the concern over the environment on the disposal of ILs are also some of the main obstacles hindering the large-scale application in the pretreatment of lignocellulosic biomass. Advantageously, ILs may have a good recyclability (Ninomiya et al 2015c ) and can be reused to reduce the cost and the impact on the environment. There are several methods to recycle ILs, and distillation of volatile solutes is always the first choice to recover the ILs due to their low vapor pressure.…”

Technoeconomic Analysis of Multiple-Stream Ethanol and Lignin Production from Lignocellulosic Biomass: Insights into the Chemical Selection and Process Integration

“…The residual ChCl:EG and H 2 SO 4 in the pretreated biomass cannot be ignored because of their toxicity to enzymes and microbes. 95,96 Moreover, Viswanathan et al 55 conducted the TEA of ethanol and diesel coproduction from industrial hemp biomass with hydrothermal pretreatment at 50% solid loading and enzymatic hydrolysis subsequently. They mentioned that glucose and xylose yields were 85 and 80%, respectively.…”

Enhancing the Thermostability of Papain by Immobilizing on Deep Eutectic Solvents-Treated Chitosan With Optimal Microporous Structure and Catalytic Microenvironment

“…They could disturb the major linkages between sugars and lignin of biomass, and extract them from the raw materials ( Feng et al, 2019 ; Tan et al, 2020 ). However, issues about DESs recovery and DESs residues in the biomass still exist ( Ninomiya et al, 2015 ). More specifically, a certain amount of solvent often remains in the biomass after the treatment, which may reduce the quality of biomass and cause solvent loss.…”

“…More specifically, a certain amount of solvent often remains in the biomass after the treatment, which may reduce the quality of biomass and cause solvent loss. Therefore, a tedious and cost-intensive washing step is required to make the treated biomass free of DES and to obtain a higher recovery yield of the solvent ( Ninomiya et al, 2015 ; Hou et al, 2017a ). Nonetheless, trace of DES may still remain in the recovered- or regenerated- biomass after washings.…”