Effect of Porphyromonas gingivalis outer membrane vesicles on gingipain-mediated detachment of cultured oral epithelial cells and immune responses

Nakao, Ryoma; Takashiba, Shogo; Kosono, Saori; Yoshida, Minoru; Watanabe, Haruo; Ohnishi, Makoto; Senpuku, Hidenobu

doi:10.1016/j.micinf.2013.10.005

Cited by 79 publications

(79 citation statements)

References 43 publications

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“…Not surprisingly, fimbrial proteins (FimA, C, D, E and Mfa1) were exclusively detected in 33277 vesicles, which is consistent with expression of these proteins in P. gingivalis 33277 cells [20,35,42]. A most striking finding was that tetratricopeptide repeat (TPR) domain proteins were not detectable in 33277 vesicles (Supplementary Table 1), while four or five TPR domain proteins were found in the vesicles from W50 and W83, even though there are eight genes encoding TPR proteins in the P. gingivais genome.…”

Section: P Gingivalis Omv Contentssupporting

confidence: 60%

“…In addition, P. gingivalis vesicles may also act as decoys to absorb specific antibodies toward the bacteria. Thus, preincubation of vesicles with serum samples from periodontitis patients was able to reduce the immune reactivity of sera against P. gingivalis cells [35]. Another likely advantage of vesiculation is that cell associated vesicles serve as an expanded outer membrane, which optimizes membrane functionality.…”

Section: Vesiculation Of P Gingivalis and Its Regulationmentioning

confidence: 99%

“…In addition to carrying the major outer membrane contents of P. gingivalis , vesicles have shown the primary characteristic features of this organism, such as induction of inflammatory responses, impairment of host cells and transmission of virulence factors into host cells [27,35]. A recent study showed that after treatment with P. gingivalis vesicles (5–10 μg ml -1 ) for 1 h, oral epithelial cells in a confluent monolayer were detached from the wells of culture plates [35].…”

Section: Virulence Of P Gingivalis Vesiclesmentioning

confidence: 99%

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Biogenesis and Function of Porphyromonas Gingivalis Outer Membrane Vesicles

Xie

2015

Future Microbiol.

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Porphyromonas gingivalis is one of the keystone pathogens associated with chronic periodontitis. All P. gingivalis strains examined thus far produce outer membrane vesicles. Recent studies have found that vesicles possess some well-known virulence factors of P. gingivalis such as adhesins, toxins and proteolytic enzymes. Carrying most of the characteristic features of their parent P. gingivalis cells, vesicles communicate with host cells and other members of microbial biofilms, resulting in the transmission of virulence factors into these host cells and the formation of pathogenic bacteria-dominated microbial communities. An in-depth understanding of both the nature and role of vesicles in the pathogenicity of P. gingivalis is both important and timely, particularly when speaking of periodontitis and its related systemic effects.

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Section: P Gingivalis Omv Contentssupporting

confidence: 60%

Section: Vesiculation Of P Gingivalis and Its Regulationmentioning

confidence: 99%

Section: Virulence Of P Gingivalis Vesiclesmentioning

confidence: 99%

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Biogenesis and Function of Porphyromonas Gingivalis Outer Membrane Vesicles

Xie

2015

Future Microbiol.

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“…The periodontal pathogens Treponema denticola and P. gingivalis produce OMVs that cause disruption and detachment of epithelial cell monolayers, which enables OMVs and their pathogenic cargo to cause further damage to the underlying tissue 35,36 . These examples highlight that OMVs are used by Gram-negative pathogens to facilitate the transfer of antigens and virulence determinants into the submucosa to maintain a chronic inflammatory state in the host.…”

Section: Disruption Of Epithelial Barrier Integritymentioning

confidence: 99%

Immune modulation by bacterial outer membrane vesicles

2015

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Gram-negative bacteria shed extracellular outer membrane vesicles (OMVs) during their normal growth both in vitro and in vivo. OMVs are spherical, bilayered membrane nanostructures that contain many components found within the parent bacterium. Until recently, OMVs were dismissed as a by-product of bacterial growth; however, findings within the past decade have revealed that both pathogenic and commensal bacteria can use OMVs to manipulate the host immune response. In this Review, we describe the mechanisms through which OMVs induce host pathology or immune tolerance, and we discuss the development of OMVs as innovative nanotechnologies.

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“…Gingipains are located on cell surfaces and in culture supernatants, and are related to extracellular vesicles (Chen et al, 2001). The gingipain-loaded outer membrane vesicles may contribute to tissue destruction in periodontal diseases by serving as a vehicle for the antigens and active proteases (Nakao et al, 2014). When separate proteinases with arginine and lysine specificity were isolated from a high molecular mass fraction of the P. gingivalis culture fluid, the arginine-and lysine-gingipains were found to contain a hemagglutinin complex.…”

Section: Discussionmentioning

confidence: 99%

Effect of Roselle Calyx Extract on Gingipain Activity, Production of Inflammatory Cytokines, and Oral Bacterial Morphology

Sulistyani¹,

Fujita²,

Nakazawa³

2016

J microb biotech food sci

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This study to investigate the effect of roselle calyx extract on gingipain activity, production of inflammatory cytokines, and oral bacterial morphology. The inhibitory effect of roselle calyx extract (RCE) on Arg- (Rgp) and Lys-gingipain (Kgp) was evaluated using synthetic substrate colorimetric assay with a 96-well microtiter plate. RCE with various concentrations was tested for determination of interleukin (IL)-6 and IL-8 produced from KB cells stimulated with heat-inactivated P. gingivalis. The production of IL-6 and IL-8 was quantified using an ELISA kit. The morphological alterations in cells of S. mutans and P. gingivalis after treatment with RCE were studied using scanning electron microscopy (SEM). RCE could inhibit both Rgp (90%) and Kgp (70%) activities significantly at concentrations lower than the minimum inhibitory concentration (MIC) (2.2 mg/mL). Furthermore, the production of IL-6 and IL-8 from KB cells stimulated with P. gingivalis was significantly inhibited by RCE at 6 h after exposure, in a dose-dependent manner. After treatment with RCE, the morphological alterations were observed in cells of S. mutans and P. gingivalis by using SEM. The ability of RCE to inhibit gingipain activity and production of inflammatory cytokines indicates that RCE could be considered for prevention and clinical treatment for periodontitis.

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Effect of Porphyromonas gingivalis outer membrane vesicles on gingipain-mediated detachment of cultured oral epithelial cells and immune responses

Cited by 79 publications

References 43 publications

Biogenesis and Function of Porphyromonas Gingivalis Outer Membrane Vesicles

Biogenesis and Function of Porphyromonas Gingivalis Outer Membrane Vesicles

Immune modulation by bacterial outer membrane vesicles

Effect of Roselle Calyx Extract on Gingipain Activity, Production of Inflammatory Cytokines, and Oral Bacterial Morphology

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