Effect of polysaccharide derived from dehulled adlay on regulating gut microbiota and inhibiting Clostridioides difficile in an in vitro colonic fermentation model

Dehulled Adlay (Coix lachryma-jobi L.) ameliorates hepatic gluconeogenesis and steatosis in streptozotocin/high-fat diet-induced diabetic rats

“…Yeh et al showed that dehulled alday displayed hypolipidemic and hypoglycemic properties in STZ/HFD-induced diabetic rats [ 19 ]. Additionally, polysaccharide derived from dehulled adlay, acted as prebiotics, promoted a healthy gut microbiota and helped maintain intestinal barrier function [ 44 ]. Furthermore, dehulled adlay fermented by Bacillus was found to improve lipid profiles, antioxidant status, and gut microflora in HFD-fed hamster [ 45 ].…”

Lactiplantibacillus plantarum-encapsulated microcapsules prepared from okra polysaccharides improved intestinal microbiota in Alzheimer’s disease mice

“…In brief, adlay polysaccharide powder (1 mg) was mixed with hydrolysis solution (1 mL) for reaction at 80°C for 2 h. The solution was mixed with 2 mg 2,3-naphthalenediamine, 1 mg iodine, and 1 mL acetic acid (stirring for 1 h) for monosaccharide fluorescent labeling ( Lin et al, 2010 ). The molecule weight was measured by diffusion ordered NMR spectroscopy (DOSY, Bruker AV600) at 600 MHz to obtain the diffusion coefficient and H1 profile ( Lee et al, 2023 ).…”

Tomato-fruit-derived extracellular vesicles inhibit Fusobacterium nucleatum via lipid-mediated mechanism

“…The in vitro colon microbial community utilized in the experiments was cultured and maintained in a simulator of intestinal microbial ecosystem, as previously described. 19 The experimental animals used in the present study were reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) in National Cheng Kung University in Taiwan (IACUC approval No. 112165).…”

“…Recently, there have been reports of C. difficile being innocuously present in the colon until intestinal microbiota imbalance arises. 19 F. nucleatum can promote cancer cell proliferation and have deleterious effects in the tumour microenvironment that appear to enable cancer cells to excrete chemotherapy drugs, thereby increasing drug resistance in cancer cells, and ultimately accelerating the development of colorectal cancer. 25 For our current study, we utilized a dose of 1 × 10 10 EV particles, which is equivalent to approximately one gram of tomato.…”