Tomato-fruit-derived extracellular vesicles inhibit Fusobacterium nucleatum via lipid-mediated mechanism

Abstract

Extracellular vesicles derived from tomato have potential on regulating gut microbiota.

Exploring new avenues of health protection: plant-derived nanovesicles reshape microbial communities

“…In addition, this was verified not only in ginger-derived nanovesicles, but also in tomato-derived nanovesicles. Gorevic et al found that tomato-derived nanovesicles were also taken up by Fusobacterium nucleatum and that this uptake process was also dependent on lipid components [ 14 ]. When lipids were removed, the uptake of tomato-derived nanovesicles by Fusobacterium nucleatum was reduced and their growth state was restored [ 14 ].…”

“…Gorevic et al found that tomato-derived nanovesicles were also taken up by Fusobacterium nucleatum and that this uptake process was also dependent on lipid components [ 14 ]. When lipids were removed, the uptake of tomato-derived nanovesicles by Fusobacterium nucleatum was reduced and their growth state was restored [ 14 ]. In turn, nanovesicles reconstituted by cholesteryl esters (CE) were able to be taken up by Fusobacterium nucleatum [ 14 ].…”

“…When lipids were removed, the uptake of tomato-derived nanovesicles by Fusobacterium nucleatum was reduced and their growth state was restored [ 14 ]. In turn, nanovesicles reconstituted by cholesteryl esters (CE) were able to be taken up by Fusobacterium nucleatum [ 14 ]. These studies confirm the important role of lipids in the recognition of PDNVs by bacteria, but more studies are needed to confirm whether other components also play an important mediating role.…”

“… Tartary buckwheat UC TBDNs significantly promoted E. coli and LGG , enhanced fecal microbial diversity, and increased short-chain fatty acid levels Human E.coli and LGG A total of 129 microRNAs were identified by sequencing analyses, including 11 newly discovered microRNAs. Target gene prediction indicated that some of the microRNAs were able to target functional genes in physiological processes related to E. coli and LGG [ 43 ] Tomato UC Promoting the growth of probiotic LGG while inhibiting the growth of the conditional pathogens C. difficile and F. nucleatum Smulator of intestinal microbial ecosystem model LGG ; C. diff and F. nucleatum The antimicrobial effect of tomato-derived nanovesicles is driven by the presence of specific lipids in the nanovesicles, as demonstrated by lipid depletion and reconstitution experiments [ 14 ] Bee pollen UC Inhibition of S. aureus biofilm formation – S. aureus – [ 67 ] Rehmannia UC + D Antimicrobial, inhibits biofilm formation LPS-treated mice E.coli miR-7972 down-regulates the expression of GPR 161, activates the Hedgehog pathway, and inhibits biofilm formation in E. coli by targeting the virulence gene stx 2 [ 44 ] Garlic UC + D Regulation of microbial community homeostasis DSS-induced gut microbiota dysbiosis – – [ 16 ] Ginger UC + D Regulation of microbial community homeostasis C57 BL/6 mouse/human …”

“…It has been shown that the key to the specific uptake of PDNVs by microbiota lies in the differences in their surface lipid fractions [ 11 ]. These vesicles, enriched with various components, have the ability to simultaneously target multiple bacterial genes, including affect bacterial metabolic functions [ 13 ], modulate pathogenic gene expression in bacteria, reduce their pathogenicity [ 12 , 14 ], and enhance the growth and survival of probiotics [ 11 , 15 ]. These biological activities lay the foundation for PDNVs to regulate microbiota homeostasis.…”