Effect of polyethylene glycol additives on structure, stability, and biocatalytic activity of ormosil sol–gel encapsulated yeast cells

Lavrova, D. G.; Kamanina, Olga A.; Мачулин, А. В.; Suzina, N. E.; Алферов, В. А.; Понаморева, О. Н.

doi:10.1007/s10971-017-4333-z

Cited by 10 publications

(8 citation statements)

References 22 publications

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“…Two reagent systems including PEG differing in molecular mass (PEG3000 in SP EG -composite and PEG400 in STP EG -composite) were used as a polymeric matrix in the study for nanocomposite synthesis and cell immobilization. This was caused by different methodological approaches in the synthesis of silicon polyethylene glycol [ 66 , 68 , 70 ]. In addition, filler particles were synthesized from a TEOS:MTES mixture (15:85 v:v ratio) when the SPEG composite was produced.…”

Section: Resultsmentioning

confidence: 99%

“…Methylotrophic yeasts possessing an effective short-chain alcohol oxidation system were used as a biological part of the composite biocatalysts. In these conditions, upon a certain ratio of silane precursors, yeast cells become the centers of formation of the so-called “cell in the shell” architectures [ 65 , 66 , 67 , 68 ]. Such shells protect the cells from environmental stress factors (heavy metal ions, UV radiation, and extreme pH values) [ 65 , 67 ], which is important for the development of industrial wastewater purification technologies.…”

Section: Introductionmentioning

confidence: 99%

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Biocompatible Silica-Polyethylene Glycol-Based Composites for Immobilization of Microbial Cells by Sol-Gel Synthesis

et al. 2023

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Biocatalysts based on the methylotrophic yeast Ogataea polymorpha VKM Y-2559 immobilized in polymer-based nanocomposites for the treatment of methanol-containing wastewater were developed. The organosilica composites with different matrix-to-filler ratios derived from TEOS/MTES in the presence of PEG (SPEG-composite) and from silicon-polyethylene glycol (STPEG-composite) differ in the structure of the silicate phase and its distribution in the composite matrix. Methods of fluorescent and scanning microscopy first confirmed the formation of an organosilica shell around living yeast cells during sol-gel bio-STPEG-composite synthesis. Biosensors based on the yeast cells immobilized in STPEG- and SPEG-composites are characterized by effective operation: the coefficient of sensitivity is 0.85 ± 0.07 mgO2 × min−1 × mmol−1 and 0.87 ± 0.05 mgO2 × min−1 × mmol−1, and the long-term stability is 10 and 15 days, respectively. The encapsulated microbial cells are protected from UV radiation and the toxic action of heavy metal ions. Biofilters based on the developed biocatalysts are characterized by high effectiveness in the utilization of methanol-rich wastewater—their oxidative power reached 900 gO2/(m3 × cycle), and their purification degree was up to 60%.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biocompatible Silica-Polyethylene Glycol-Based Composites for Immobilization of Microbial Cells by Sol-Gel Synthesis

et al. 2023

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“…The sensitivity coefficient was determined as the tangent of the slope of the rectilinear section of the calibration function ( Table 1 ). Characteristics of the biosensor obtained on the basis of P. yeei bacteria encapsulated in an organosilicon sol–gel matrix with a precursor ratio of 50 vol.% DEDMS and 50 vol.% TEOS were compared with the previously studied bioreceptor element based on the same bacteria, where the MTES precursor was used instead of DEDMS [ 7 ].…”

Section: Resultsmentioning

confidence: 99%

“…The replacement of methyltriethoxysilane [ 7 ] with diethoxydimethylsilane led to a narrower range of determined BOD contents and a lower limit of determined concentrations. Presumably, this is due to the peculiarity of the DEDMS structure, which has a smaller number of Si-O– bonds, which leads to the formation of a material with a flexible and flat structure.…”

Section: Resultsmentioning

confidence: 99%

“…Over the past ten years, our research team has been conducting research on the production of the porous hybrid biocatalysts using sol–gel technology. The simple and mild conditions for sol–gel synthesis were proposed, under which the spontaneous formation of an organosilicate capsule around methylotrophic yeast cells [ 7 ] occurs when tetraethoxysilane and methyltriethoxysilane are used as silane precursors [ 8 ]. The processes of the utilization of wastewater containing methanol with oxygen saturation [ 9 ] and without oxygen saturation [ 10 ] have been studied.…”

Section: Introductionmentioning

confidence: 99%

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The Use of Diethoxydimethylsilane as the Basis of a Hybrid Organosilicon Material for the Production of Biosensitive Membranes for Sensory Devices

et al. 2022

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Biomembranes based on an organosilica sol–gel matrix were used to immobilize bacteria Paracoccus yeei VKM B-3302 as part of a biochemical oxygen demand (BOD) biosensor. Diethoxydimethylsilane (DEDMS) and tetraethoxysilane (TEOS) were used as precursors to create the matrix in a 1:1 volume ratio. The use of scanning electron microscopy (SEM) and the low-temperature nitrogen adsorption method (BET) showed that the sol–gel matrix forms a capsule around microorganisms that does not prevent the exchange of substrates and waste products of bacteria to the cells. The use of DEDMS as part of the matrix made it possible to increase the sensitivity coefficient of the biosensor for determining BOD by two orders of magnitude compared to a biosensor based on methyltriethoxysilane (MTES). Additionally, the long-term stability of the bioreceptor increased to 68 days. The use of such a matrix neutralized the effect of heavy metal ions on the microorganisms’ catalytic activity in the biosensor. The developed biosensor was used to analyze water samples from water sources in the Tula region (Russia).

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New insights into the predicament of DFT assisted optimized energy, stability and distortions of optimized topologies of some novel complexes of Zirconium (IV) and enhancement of antimicrobial potential

Sharma¹,

Kumar

et al. 2019

Applied Organom Chemis

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A comprehensive density functional theory (DFT/B3LYP method) investigation was done to study the generation, optimized topologies, bonding, reactivity, stability, distortions and enhancement of antimicrobial potential of a number of organic–inorganic hybrid complexes of zirconium (IV). Zirconium (IV) complexes having the general formulae ZrL(Cl)2 and Zr(L)2 were prepared by the reaction of zirconium tetrachloride with dipotassium salts of sterically constrained oximes of heterocyclic β‐diketones in different stoichiometric ratios in refluxing anhydrous THF. Plausible topologies of these complexes were suggested with the aid of physico‐chemical and spectral studies. The optimized topologies of the complexs of the types ZrL(Cl)2 and Zr(L)2 were given by comprehensive DFT investigation. The molecular orbitals, molecular electrostatic potential, chemical reactivity, energy optimization, stability and distortions of these newly generated complexes were studied by DFT. These complexes were screened against bacterial isolate E. Coli and fungal isolates viz. A. Niger and T. Reesei. The chemical reactivity of these complexes is corroborated by small energy gap. Few principal quantum chemical descriptors were correlated with the enhancement of antimicrobial potential of these sterically constrained complexes of zirconium (IV). The presence of ‐CH2CH3, ‐C6H5 and –C6H4Cl (p) groups in complexes of the types ZrL(Cl)2 and Zr(L)2 provides an opportunity to examine the effect on optimized energy, stability and distortions of optimized topologies.

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Effect of polyethylene glycol additives on structure, stability, and biocatalytic activity of ormosil sol–gel encapsulated yeast cells

Cited by 10 publications

References 22 publications

Biocompatible Silica-Polyethylene Glycol-Based Composites for Immobilization of Microbial Cells by Sol-Gel Synthesis

Biocompatible Silica-Polyethylene Glycol-Based Composites for Immobilization of Microbial Cells by Sol-Gel Synthesis

The Use of Diethoxydimethylsilane as the Basis of a Hybrid Organosilicon Material for the Production of Biosensitive Membranes for Sensory Devices

New insights into the predicament of DFT assisted optimized energy, stability and distortions of optimized topologies of some novel complexes of Zirconium (IV) and enhancement of antimicrobial potential

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