Effect of poly (γ-glutamic acid)/tricalcium phosphate (γ-PGA/TCP) composite for dentin remineralization &lt;i&gt;in vitro &lt;/i&gt;

Su, Qingqing; Zhang, Chao; Mai, Sui; Lin, Huancai; Zhi, Qinghui

doi:10.4012/dmj.2019-324

Cited by 6 publications

(4 citation statements)

References 47 publications

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“…This result may be due to the adhesion of C. albicans on the bovine dentin surface influencing the surface roughness; the detection of surface roughness by CLSM can be easily affected by the environment, which could have led to the lack of a significant difference between the two groups. SEM images showed that the bovine dentin surface morphology of the S. mutans MV-treated group had more dentin tubule exposure and broken dentin tubules, indicating the erosion of peritubular dentin and consistent with previous research reports ( Poggio et al., 2013 ; Su et al., 2021 ). Altogether, these findings provide evidence that S. mutans MVs increase bovine dentin demineralization provoked by C. albicans biofilms.…”

Section: Discussionsupporting

confidence: 91%

Streptococcus Mutans Membrane Vesicles Enhance Candida albicans Pathogenicity and Carbohydrate Metabolism

Cui

Cao

et al. 2022

Front. Cell. Infect. Microbiol.

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Streptococcus mutans and Candida albicans, as the most common bacterium and fungus in the oral cavity respectively, are considered microbiological risk markers of early childhood caries. S. mutans membrane vesicles (MVs) contain virulence proteins, which play roles in biofilm formation and disease progression. Our previous research found that S. mutans MVs harboring glucosyltransferases augment C. albicans biofilm formation by increasing exopolysaccharide production, but the specific impact of S. mutans MVs on C. albicans virulence and pathogenicity is still unknown. In the present study, we developed C. albicans biofilms on the surface of cover glass, hydroxyapatite discs and bovine dentin specimens. The results showed that C. albicans can better adhere to the tooth surface with the effect of S. mutans MVs. Meanwhile, we employed C. albicans biofilm-bovine dentin model to evaluate the influence of S. mutans MVs on C. albicans biofilm cariogenicity. In the S. mutans MV-treated group, the bovine dentin surface hardness loss was significantly increased and the surface morphology showed more dentin tubule exposure and broken dentin tubules. Subsequently, integrative proteomic and metabolomic approaches were used to identify the differentially expressed proteins and metabolites of C. albicans when cocultured with S. mutans MVs. The combination of proteomics and metabolomics analysis indicated that significantly regulated proteins and metabolites were involved in amino acid and carbohydrate metabolism. In summary, the results of the present study proved that S. mutans MVs increase bovine dentin demineralization provoked by C. albicans biofilms and enhance the protein and metabolite expression of C. albicans related to carbohydrate metabolism.

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Section: Discussionsupporting

confidence: 91%

Streptococcus Mutans Membrane Vesicles Enhance Candida albicans Pathogenicity and Carbohydrate Metabolism

Cui

Cao

et al. 2022

Front. Cell. Infect. Microbiol.

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“…S. Vyavhare et al reported a similar trend for comparing different remineralizing agents on surface microhardness recovery. The reported SMHR% for nano-HAp (10%), CPP-ACP (10%), NaF (1000 ppm), and deionized water (negative control) after 6 days of pH cycling were 37.06 ± 7.32%, 18.17 ± 7.70%, 37.68 ± 7.06%, and 16.52 ± 8.77%, respectively [ 53 ]. Furthermore, γ-PGA/tricalcium phosphate was recently reported to be more effective than CPP-ACP at enhancing the microhardness of bovine dentin samples after 2 weeks of pH cycling remineralization [ 53 ].…”

Section: Resultsmentioning

confidence: 99%

“…The reported SMHR% for nano-HAp (10%), CPP-ACP (10%), NaF (1000 ppm), and deionized water (negative control) after 6 days of pH cycling were 37.06 ± 7.32%, 18.17 ± 7.70%, 37.68 ± 7.06%, and 16.52 ± 8.77%, respectively [ 53 ]. Furthermore, γ-PGA/tricalcium phosphate was recently reported to be more effective than CPP-ACP at enhancing the microhardness of bovine dentin samples after 2 weeks of pH cycling remineralization [ 53 ]. S. Huang et al reported 35.4% of SMHR in 144 min of treatment time in enamel remineralization under the pH-cycling model for dental caries, using nano-HAp combined with Galla chinensis (GCE) [ 54 ].…”

Section: Resultsmentioning

confidence: 99%

Rehardening and the Protective Effect of Gamma-Polyglutamic Acid/Nano-Hydroxyapatite Paste on Surface-Etched Enamel

et al. 2021

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Many revolutionary approaches are on the way pertaining to the high occurrence of tooth decay, which is an enduring challenge in the field of preventive dentistry. However, an ideal dental care material has yet to be fully developed. With this aim, this research reports a dramatic enhancement in the rehardening potential of surface-etched enamels through a plausible synergistic effect of the novel combination of γ-polyglutamic acid (γ-PGA) and nano-hydroxyapatite (nano-HAp) paste, within the limitations of the study. The percentage of recovery of the surface microhardness (SMHR%) and the surface parameters for 9 wt% γ-PGA/nano-HAp paste on acid-etched enamel were investigated with a Vickers microhardness tester and an atomic force microscope, respectively. This in vitro study demonstrates that γ-PGA/nano-HAp treatment could increase the SMHR% of etched enamel to 39.59 ± 6.69% in 30 min. To test the hypothesis of the rehardening mechanism and the preventive effect of the γ-PGA/nano-HAp paste, the surface parameters of mean peak spacing (Rsm) and mean arithmetic surface roughness (Ra) were both measured and compared to the specimens subjected to demineralization and/or remineralization. After the treatment of γ-PGA/nano-HAp on the etched surface, the reduction in Rsm from 999 ± 120 nm to 700 ± 80 nm suggests the possible mechanism of void-filling within a short treatment time of 10 min. Furthermore, ΔRa-I, the roughness change due to etching before remineralization, was 23.15 ± 3.23 nm, while ΔRa-II, the roughness change after remineralization, was 11.99 ± 3.90 nm. This statistically significant reduction in roughness change (p < 0.05) implies a protective effect against the demineralization process. The as-developed novel γ-PGA/nano-HAp paste possesses a high efficacy towards tooth microhardness rehardening, and a protective effect against acid etching.

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“…The results showed that charged groups in collagen provide nucleation sites for inducing apatite nucleation. The experimental results of Su et al (2021) suggested that type I collagen has a complex and ordered structure and plays a positive role in guiding the penetration of minerals into nucleation. Nevertheless, whether collagen alone can form intrafibrillar mineralization remains controversial.…”

Section: Importance Of Collagen In Biomineralizationmentioning

confidence: 99%

Guided tissue remineralization and its effect on promoting dentin bonding

Yao

Chen

et al. 2022

Front. Mater.

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With the deepening of research on condensed matter chemistry, artificially guided demineralized dentin remineralization has changed from a classical remineralization pathway of the thermodynamic deposition mode to a biomimetic mineralization mode. This new mode is more consistent with the biological mineralization process. The biomimetic mineralization model can successfully simulate natural mineralization and restore the microstructure and mechanical properties of demineralized dentin. Therefore, it has a good application value in the treatment of caries and dentin hypersensitivity and adhesive restorations. This paper analyzes the principles of guided tissue remineralization and describes new research findings related to the classical mineralization model and the novel biomaterials developed using the biomimetic mineralization mode in detail. It also describes the application of these principles to improve the dentin bonding system. It thus shares the new findings in guided tissue remineralization applied to dentin bonding systems. Finally, the existing problems in this field and future development directions are proposed.

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Effect of poly (γ-glutamic acid)/tricalcium phosphate (γ-PGA/TCP) composite for dentin remineralization <i>in vitro </i>

Cited by 6 publications

References 47 publications

Streptococcus Mutans Membrane Vesicles Enhance Candida albicans Pathogenicity and Carbohydrate Metabolism

Streptococcus Mutans Membrane Vesicles Enhance Candida albicans Pathogenicity and Carbohydrate Metabolism

Rehardening and the Protective Effect of Gamma-Polyglutamic Acid/Nano-Hydroxyapatite Paste on Surface-Etched Enamel

Guided tissue remineralization and its effect on promoting dentin bonding

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