Effect of Pleurotus ostreatus and Trametes versicolor on triclosan biodegradation and activity of laccase and manganese peroxidase enzymes

Mallak, Ayda Maadani; Lakzian, Amir; Khodaverdi, Elham; Haghnia, G H; Mahmoudi, Shima

doi:10.1016/j.micpath.2020.104473

Cited by 13 publications

(4 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, we do not know much about the processes occurring in natural conditions with the participation of microorganisms. Cunninghamella elegans and Trametes versicolor are well known for their ability to eliminate different hazardous pollutants, including drugs, pesticides, dyes and personal care products [ 41 , 42 , 43 , 44 ]. Despite the fact that chloroxylenol is a micropollutant occurring in the natural environment, there is still little information available about its elimination and fate.…”

Section: Resultsmentioning

confidence: 99%

Biodegradation of Chloroxylenol by Cunninghamella elegans IM 1785/21GP and Trametes versicolor IM 373: Insight into Ecotoxicity and Metabolic Pathways

Nowak

Zawadzka

Szemraj

et al. 2021

IJMS

View full text Add to dashboard Cite

Chloroxylenol (PCMX) is applied as a preservative and disinfectant in personal care products, currently recommended for use to inactivate the SARS-CoV-2 virus. Its intensive application leads to the release of PCMX into the environment, which can have a harmful impact on aquatic and soil biotas. The aim of this study was to assess the mechanism of chloroxylenol biodegradation by the fungal strains Cunninghamella elegans IM 1785/21GP and Trametes versicolor IM 373, and investigate the ecotoxicity of emerging by-products. The residues of PCMX and formed metabolites were analysed using GC-MS. The elimination of PCMX in the cultures of tested microorganisms was above 70%. Five fungal by-products were detected for the first time. Identified intermediates were performed by dechlorination, hydroxylation, and oxidation reactions catalysed by cytochrome P450 enzymes and laccase. A real-time quantitative PCR analysis confirmed an increase in CYP450 genes expression in C. elegans cells. In the case of T. versicolor, spectrophotometric measurement of the oxidation of 2,20-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) showed a significant rise in laccase activity during PCMX elimination. Furthermore, with the use of bioindicators from different ecosystems (Daphtoxkit F and Phytotoxkit), it was revealed that the biodegradation process of PCMX had a detoxifying nature.

show abstract

Section: Resultsmentioning

confidence: 99%

Biodegradation of Chloroxylenol by Cunninghamella elegans IM 1785/21GP and Trametes versicolor IM 373: Insight into Ecotoxicity and Metabolic Pathways

Nowak

Zawadzka

Szemraj

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Next, 0.2 mL of the supernatant was thoroughly mixed with 5 mL of Coomassie Brilliant Blue G-250 staining solution and left to stand for 5 min, and the OD595 nm was measured. The laccase and manganese peroxidase (MnP) activities were measured according to the method described before using crude enzyme extract . The crude extract was generated as follows: 8 g of culture medium from the same region of the fungal bag was collected and mixed with 32 mL of 1× phosphate-buffered saline (PBS).…”

Section: Methodsmentioning

confidence: 99%

“…The laccase and manganese peroxidase (MnP) activities were measured according to the method described before using crude enzyme extract. 43 The crude extract was generated as follows: 8 g of culture medium from the same region of the fungal bag was collected and mixed with 32 mL of 1× phosphate-buffered saline (PBS). The mixture was shaken at 125 rpm at 25 °C for 30 min and subsequently centrifuged at 6000 g for 10 min to obtain the supernatant.…”

Section: ■ Introductionmentioning

confidence: 99%

Comparative Proteomic Analysis Reveals Candidate Pathways Related to the Effect of Different Light Qualities on the Development of Mycelium and Fruiting Body of Pleurotus ostreatus

Zhu,

Su,

et al. 2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

Light affects the morphology and physiology of Pleurotus ostreatus. However, the underlying molecular mechanism of this effect remains unclear. In this study, a label-free comparative proteomic analysis was conducted to investigate the global protein expression profile of the mycelia and fruiting bodies of P. ostreatus PH11 growing under four different light quality treatments. Among all the 2234 P. ostreatus proteins, 1349 were quantifiable under all tested conditions. A total of 1100 differentially expressed proteins were identified by comparing the light group data with those of the darkness group. GO and KEGG enrichment analyses indicated that the oxidative phosphorylation, proteasome, and mRNA surveillance pathways were the most related pathways under the light condition. qRT-PCR verified that the expression of the white collar 1 protein was significantly enhanced under white light. Additionally, glutamine synthetase and aldehyde dehydrogenase played important roles during light exposure. This study provides valuable insight into the P. ostreatus light response mechanism, which will lay the foundation for improved cultivation.

show abstract

“…Lentinus squarrosulus has the maximum microbial removal from the river water sample, with 100% removal, followed by Pleurotus ostreatus (99%) and Trametes Versicolor (24–28%) [ 123 , 124 , 126 ]. The laccase concentration, which impacts the potential and ligninolytic enzymatic activity, is the difference in performance between these species [ 134 ]. It is stated that 0.25% (w/w) of the polysaccharide from the macrofungi can be the minimum inhibitory concentration (MIC) for phatogenic microbes such as E.coli in water [ 135 ].…”

Section: Water Treatmentmentioning

confidence: 99%

Role of ascomycete and basidiomycete fungi in meeting established and emerging sustainability opportunities: a review

et al. 2022

View full text Add to dashboard Cite

Fungal biomass is the future’s feedstock. Non-septate Ascomycetes and septate Basidiomycetes, famously known as mushrooms, are sources of fungal biomass. Fungal biomass, which on averagely comprises about 34% protein and 45% carbohydrate, can be cultivated in bioreactors to produce affordable, safe, nontoxic, and consistent biomass quality. Fungal-based technologies are seen as attractive, safer alternatives, either substituting or complementing the existing standard technology. Water and wastewater treatment, food and feed, green technology, innovative designs in buildings, enzyme technology, potential health benefits, and wealth production are the key sectors that successfully reported high-efficiency performances of fungal applications. This paper reviews the latest technical know-how, methods, and performance of fungal adaptation in those sectors. Excellent performance was reported indicating high potential for fungi utilization, particularly in the sectors, yet to be utilized and improved on the existing fungal-based applications. The expansion of fungal biomass in the industrial-scale application for the sustainability of earth and human well-being is in line with the United Nations’ Sustainable Development Goals.

show abstract

Effect of Pleurotus ostreatus and Trametes versicolor on triclosan biodegradation and activity of laccase and manganese peroxidase enzymes

Cited by 13 publications

References 39 publications

Biodegradation of Chloroxylenol by Cunninghamella elegans IM 1785/21GP and Trametes versicolor IM 373: Insight into Ecotoxicity and Metabolic Pathways

Biodegradation of Chloroxylenol by Cunninghamella elegans IM 1785/21GP and Trametes versicolor IM 373: Insight into Ecotoxicity and Metabolic Pathways

Comparative Proteomic Analysis Reveals Candidate Pathways Related to the Effect of Different Light Qualities on the Development of Mycelium and Fruiting Body of Pleurotus ostreatus

Role of ascomycete and basidiomycete fungi in meeting established and emerging sustainability opportunities: a review

Contact Info

Product

Resources

About