Comparative Proteomic Analysis Reveals Candidate Pathways Related to the Effect of Different Light Qualities on the Development of Mycelium and Fruiting Body of Pleurotus ostreatus

Abstract

Light affects the morphology and physiology of Pleurotus ostreatus. However, the underlying molecular mechanism of this effect remains unclear. In this study, a label-free comparative proteomic analysis was conducted to investigate the global protein expression profile of the mycelia and fruiting bodies of P. ostreatus PH11 growing under four different light quality treatments. Among all the 2234 P. ostreatus proteins, 1349 were quantifiable under all tested conditions. A total of 1100 differentially expressed proteins were identified by comparing the light group data with those of the darkness group. GO and KEGG enrichment analyses indicated that the oxidative phosphorylation, proteasome, and mRNA surveillance pathways were the most related pathways under the light condition. qRT-PCR verified that the expression of the white collar 1 protein was significantly enhanced under white light. Additionally, glutamine synthetase and aldehyde dehydrogenase played important roles during light exposure. This study provides valuable insight into the P. ostreatus light response mechanism, which will lay the foundation for improved cultivation.

Exopolysaccharide Production in Submerged Fermentation of Pleurotus ostreatus under Red and Green Light

“…A recent study of P. ostreatus mycelia and basidioma proteomic analysis under different light wavelengths revealed that basidioma and mycelia present different responses under light, with white and blue light significantly promoting basidioma growth while red light and darkness significantly inhibited this growth. Concerning mycelia production in Petri dishes, the same study indicates that mycelia production is favored under white light while red light and darkness present morphological differentiation [40]. A study of the strain Phanerochaete chrysosporium summarizes that biomass production is favored under conditions of yellow and green light [41].…”