Effect of Platelet Rich Plasma and Fibrin Sealant on Facial Nerve Regeneration in a Rat Model

Farrag, Tarik Y.; Lehar, Mohamed; Verhaegen, Pauline D. H. M.; Carson, Kathryn A.; Byrne, Patrick J.

doi:10.1097/01.mlg.0000249726.98801.77

Cited by 158 publications

(138 citation statements)

References 14 publications

Supporting

Mentioning

124

Contrasting

Unclassified

Order By: Relevance

“…The use of autologous PRP, which serves as a source of growth factors, has gained wide acceptance in several surgical applications such as bone regeneration in periodontal and maxillofacial surgery, orthopaedic surgery, otolaryngology and plastic surgery [8,15,16,19]. Recently, some studies had suggested that PRP stimulate either cell proliferation or matrix metabolism by articular chondrocytes in vitro [1].…”

Section: Discussionmentioning

confidence: 99%

The regenerative effect of platelet-rich plasma on healing in large osteochondral defects

Sun

Zhang

Chen

et al. 2009

International Orthopaedics (SICOT)

240

178

View full text Add to dashboard Cite

Platelet-rich plasma (PRP), a platelet concentrate made of autogenous blood, has been used to improve bone and soft tissue defect healing in recent years. The aim of this study was to assess the effect of PRP on articular cartilage defects in a rabbit model. Forty-eight osteochondral defects created in the femoropatellar groove were (a) left untreated, (b) treated with autogenous PRP in a polylactic-glycolic acid (PLGA), or (c) with PLGA alone. Platelets were enriched 5.12-fold compared to normal blood in the PRP. After four and 12 weeks, the explanted tissue specimens were assessed by macroscopic examination, micro-computed tomography, and histological evaluation. Macroscopic examination, micro-computed tomography and histology of the newly formed cartilage and bone in the defect differ significantly between the PRPtreated and the untreated groups, and stimulatory effect of PRP on osteochondral formation was observed. In conclusion, PRP in PLGA improves osteochondral healing in a rabbit model.

show abstract

Section: Discussionmentioning

confidence: 99%

The regenerative effect of platelet-rich plasma on healing in large osteochondral defects

Sun

Zhang

Chen

et al. 2009

International Orthopaedics (SICOT)

240

178

View full text Add to dashboard Cite

show abstract

“…The platelets that constitute PRP are able to release various growth factors that are essential for the healing of lesions, such as the three (91,92) . The platelets are also responsible for the synthesis and storage of BDNF (93) .…”

Section: Collagen and Laminin As Carriersmentioning

confidence: 99%

“…PRP has been used by areas such as oral and bucomaxillofacial surgery for some time (94)(95)(96) , and it has aroused considerable interest in cosmetic (97,98) and orthopedic (99,100) surgery. In experimental studies, PRP was used in peripheral nerve lesions, promoting remyelination in the facial (91) and sciatic nerve of rats (8) . The application of PRP increases the number of nerve fibers after peripheral nerve lesions, and can produce a neurotrophic effect, stimulating the proliferation of Schwann cells and myelination, important components during peripheral nerve repair (6,101) .…”

Section: Collagen and Laminin As Carriersmentioning

confidence: 99%

Regeneração de nervos periféricos: terapia celular e fatores neurotróficos

Sebben¹,

Lichtenfels²,

Silva

2011

Rev. bras. ortop.

View full text Add to dashboard Cite

Peripheral nerve trauma results in functional loss in the innervated organ, and recovery without surgical intervention is rare. Many surgical techniques can be used for nerve repair. Among these, the tubulization technique can be highlighted: this allows regenerative factors to be introduced into the chamber. Cell therapy and tissue engineering have arisen as an alternative for stimulating and aiding peripheral nerve regeneration. Therefore, the aim of this review was to provide a survey and analysis on the results from experimental and clinical studies that used cell therapy and tissue engineering as tools for optimizing the regeneration process. The articles used came from the LILACS, Medline and SciELO scientific databases. Articles on the use of stem cells, Schwann cells, growth factors, collagen, laminin and platelet-rich plasma for peripheral nerve repair were summarized over the course Rev Bras Ortop. 2011;46(6):643-49 of the review. Based on these studies, it could be concluded that the use of stem cells derived from different sources presents promising results relating to nerve regeneration, because these cells have a capacity for neuronal differentiation, thus demonstrating effective functional results. The use of tubes containing bioactive elements with controlled release also optimizes the nerve repair, thus promoting greater myelination and axonal growth of peripheral nerves. Another promising treatment is the use of platelet-rich plasma, which not only releases growth factors that are important in nerve repair, but also serves as a carrier for exogenous factors, thereby stimulating the proliferation of specific cells for peripheral nerve repair.

show abstract

“…Among 165], collagen gel tubes [166], bioartificial nerve graft seeded with Schwann cells [167], Schwann cells modified to over-express specific growth factors [168][169][170], conduits of synthetic materials [147,153,171,172], the infusion of antibodies [173], gradients of factors within tubes [174], allographs [175], factors that induce inflammation [149,176], pseudo nerves [177], alginate gel [178], biodegradable polymer tubes [179], tubes filled with platelet-rich plasma [180][181][182], and arteries, veins and muscle tubes [160, 183,184]. None of these techniques induces more effective axon regeneration than autologous sensory nerve grafts [20,31,[185][186][187][188].…”

Section: Alternative Tested Techniques For Bridging Nerve Gapsmentioning

confidence: 99%

Promoting Axon Regeneration and Neurological Recovery Following Traumatic Peripheral Nerve Injuries

Kuffler¹

2015

Int J Neurorehabilitation Eng

View full text Add to dashboard Cite

Repairing Peripheral Nerves following Traumas Nerve crushWithin several days of crushing a peripheral nerve, the injured axons begin to regenerate through their distal pathway until they reach their original targets with which they restore neurological function. The larger the number of axons that regenerate through the distal nerve, the greater the extent of neurological recovery [1][2][3]. The number of axons that regenerate, and thus neurological recovery, is influenced by the physiological state of the distal denervated nerve pathway [4][5][6][7].Following a peripheral nerve crush, the injured axons begin to regenerate within 2 days of injury [8]. They may regenerate to their targets through their original pathway in association with and promoted by its Schwann cells, the neurotrophic factors they release, and the Schwann cell extracellular matrix [1,4,[9][10][11][12][13][14]. Thus, the distal nerve provides a simple means for promoting and directing the regenerating axons to their original targets [15][16][17][18]. However, when a nerve has a gap, the gap must be bridged with a material that is permissive to and promotes axon regeneration across the entire gap to reach the distal nerve stump, through which the axons must then be able to regenerate.The Schwann cells in the denervated distal nerve release neurotrophic factors that promote axon regeneration, but also release extracellular matrix components that promote and inhibit axon regeneration [19][20][21][22][23][24][25][26]. Thus, regeneration through the distal nerve is a balance of the influences of factors that both promote and inhibit regeneration. If the Schwann cells are present, such as after a simple nerve crush, virtually 100% of the axons regenerate and they innervate all the denervated synaptic sites. If the Schwann cells within the distal nerve pathway are killed, leaving only the extracellular matrix intact, the number of axons that regenerates to their targets decreases by 94%. This is because the factors required to trigger the regenerating axons to branch at extracellular matrix branch points are missing, axons do not branch, and therefore each axon reinnervates only a single denervated muscle fiber. Thus, Schwann cells along the distal nerve pathway and a AbstractFollowing a peripheral nerve crush, or when a peripheral the nerve is transected and the nerve stumps anastomosed, neurological recovery is generally excellent. This is because the axons merely have to regenerate through the distal portion of the nerve through the existing extracellular matrix of the denervated Schwann cells that direct and promote them to their distant denervated motor and sensory targets. However, when a length of a peripheral nerve is destroyed, and anastomosis is not possible, the standard surgical repair technique is to graft a length/s of autologous sensory nerve into the gap. Neurological recovery is generally good if the nerve gap is <2 cm in length, the repair is performed <4 months post trauma, and the patients are <25 years of age. Because the nerve ...

show abstract

Effect of Platelet Rich Plasma and Fibrin Sealant on Facial Nerve Regeneration in a Rat Model

Cited by 158 publications

References 14 publications

The regenerative effect of platelet-rich plasma on healing in large osteochondral defects

The regenerative effect of platelet-rich plasma on healing in large osteochondral defects

Regeneração de nervos periféricos: terapia celular e fatores neurotróficos

Promoting Axon Regeneration and Neurological Recovery Following Traumatic Peripheral Nerve Injuries

Contact Info

Product

Resources

About