Effect of platelet concentration in platelet-rich plasma on peri-implant bone regeneration

Weibrich, Gernot; Hansen, Torsten; Kleis, Wilfried K G; Buch, R. S. R.; Hitzler, W

doi:10.1016/j.bone.2003.12.010

Cited by 531 publications

(414 citation statements)

References 9 publications

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“…However, Weibrich et al 14 (implants) while studying different levels of PRP concentration, found positive results with concentrations lower than six times, and observed that the use of highly concentrated PRP (6-11 times) inhibits the bone repair. The technique used for the production of the PRP in the current study was capable to produce mean platelets concentrations higher than six times the concentrations verified in the peripheral venous blood.…”

Section: Discussionmentioning

confidence: 99%

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Effects of a highly concentrated platelet-rich plasma on the bone repair using non-critical defects in the calvaria of rabbits

et al. 2010

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PURPOSE: To verify the effect of highly concentrated platelet-rich plasma (hPRP) in the pathways of bone repair using non-critical defects in the calvaria of rabbits. METHODS: The hPRP was produced from collected venous blood of 21 rabbits. Four non-critical defects of 8 mm in diameter were created on the calvaria of each animal. The defects were all treated differently: autogenous particled bone (APB, group 1), autogenous particled bone associated with hPRP (APB + hPRP, group 2), isolated hPRP (group 3), and blood clot (control, group 4). Animals were submitted to euthanasia on the 2nd, 4th and 6th week postoperatively. Histological and histomorphometric analysis were carried through. RESULTS: After two weeks, groups 1 and 2 were in more advanced stage of repair than 3 and 4. At this period, comparing the groups 1 and 2, no significant differences were found between both, which also happened between the groups 3 and 4. However, after four and six weeks, the group 1 showed a more advanced stage of repair among all the other studied groups, while group 2 was in more advanced signs of bone repair than groups 3 and 4. Comparing groups 3 and 4, after four and six weeks, the least advanced stage of bone repair was found to be within group 3. CONCLUSION: The use of a highly concentrated PRP was considered prejudicial to the repair of non-critical defects in the calvaria of rabbits, either in the association of autogenous particled bone, when compared to autogenous particled bone alone, or in its isolated form, when compared to blood clot (control).

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Section: Discussionmentioning

confidence: 99%

“…Although there is still no consensus about the ideal platelet concentration that could optimize the tissue repair process, some in vivo and in vitro studies suggest that a PRP highly concentrated could even be harmful to the repair [13][14][15] .…”

Section: Introductionmentioning

confidence: 99%

Effects of a highly concentrated platelet-rich plasma on the bone repair using non-critical defects in the calvaria of rabbits

et al. 2010

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“…for 15 min. The platelet button was resuspended in platelet poor plasma (PPP), so as to obtain a platelet concentrate equal to 1 Â 10 6 /ml plasma as suggested by Weibrich et al 13 For platelet activation, bovine thrombin (Sigma) was reconstitutedwithCaCl 2 0.025M(Biomerieux,Rome,Italy) to a concentration of 1,000 U/ml, sterilized by filtration and added to the platelet suspension, in the proportion 1 part of thrombin þ9 parts platelet concentrate. It was stirred and, in a few seconds, a gel formed.…”

Section: Platelet-rich Plasma Gel (Prp)mentioning

confidence: 99%

In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet‐rich plasma, and freeze‐dried bone allografts, alone and in combination

Dallari

Fini

Stagni

et al. 2006

Journal Orthopaedic Research

155

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The repair of confined trabecular bone defects in rabbits treated by autologous bone marrow stromal cells (BMSC), platelet-rich plasma (PRP), freeze-dried bone allografts (FDBA) alone and in combination (BMSC þ PRP; FDBA þ BMSC; FDBA þ PRP; FDBA þ PRP þ BMSC) was compared. A critical size defect was created in the distal part of the femurs of 48 adult rabbits. Histology and histomorphometry were used in the evaluation of healing at 2, 4, and 12 weeks after surgery. The healing rate (%) was calculated by measuring the residual bone defect area. Architecture of the newly formed bone was compared with that of bone at the same distal femur area of healthy rabbits. The defect healing rate was higher in PRP þ BMSC, FDBA þ PRP, FDBA þ BMSC, and FDBA þ PRP þ BMSC treatments, while lower values were achieved with PRP treatment at all experimental times. The highest bone-healing rate at 2 weeks was achieved with FDBA þ PRP þ BMSC treatment, which resulted significantly different from PRP (p < 0.05) and BMSC (p < 0.05) treatments. At 4 weeks, the bone-healing rate increased except for PRP treatment. Finally, the bone-healing rate of FDBA þ PRP, FDBA þ BMSC, and FDBA þ PRP þ BMSC was significantly higher than that of PRP at 12 weeks (p < 0.05). At 12 weeks, significant differences still existed between PRP, BMSC, and FDBA groups and normal bone (p < 0.05). These results showed that the combination of FDBA, BMSC and PRP permitted an acceleration in bone healing and bone remodeling processes. ß

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“…Un sistema que ofrezca un mayor número absoluto de plaquetas no necesariamente será mejor, sino que debe atenerse a parámetros mínimos y máximos que producen un efecto óptimo en la reparación ósea, lo cual se ha demostrado que se consigue con concentraciones de plaquetas entre 503.000 y 1.729.000 plaquetas/uL de PRP 15 . Concentraciones por debajo de 380.000 plaquetas/uL tendrían un efecto sub-óptimo y concentraciones por encima de 1.800.000 plaquetas/uL podrían tener un efecto paradójico en el cual inhibirían la reparación 15 .…”

Section: Preparación Del Prpunclassified

Uso del plasma rico en plaquetas en cirugía ligamentosa de rodilla

Ximena

et al. 2013

Rev. méd. Chile

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Effect of platelet concentration in platelet-rich plasma on peri-implant bone regeneration

Cited by 531 publications

References 9 publications

Effects of a highly concentrated platelet-rich plasma on the bone repair using non-critical defects in the calvaria of rabbits

Effects of a highly concentrated platelet-rich plasma on the bone repair using non-critical defects in the calvaria of rabbits

In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet‐rich plasma, and freeze‐dried bone allografts, alone and in combination

Uso del plasma rico en plaquetas en cirugía ligamentosa de rodilla

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