Effect of Plant Polyphenols on Ischemia‐Reperfusion Injury of the Isolated rat Heart and Vessels

Abstract

In the present study, we investigated the potential protective effect of selected natural substances in a rat model of heart and mesenteric ischemia-reperfusion (I/R). Experiments were performed on isolated Langendorff-perfused rat hearts, subjected to 30-min global ischemia, followed by 30-min reperfusion. Arbutin, curcumin, rosmarinic acid and extract of Mentha x villosa were applied in the concentration of 1 × 10⁻⁵ mol/l 10 min before the onset of ischemia and during reperfusion, through the perfusion medium. Mesenteric ischemia was induced by clamping the superior mesenteric artery (SMA) for 60 min, subsequent reperfusion lasted 30 min. Production of reactive oxygen species (ROS) by SMA ex vivo was determined by luminol-enhanced chemiluminiscence (CL). The effect of the substances was tested after their incubation with tissue. Curcumin and extract of Mentha x villosa were found to be the most effective in reducing reperfusion-induced dysrhythmias--ventricular tachycardia and fibrillation. This effect was accompanied by bradycardic effect. The mesenteric I/R induced an increase in CL in vascular tissue which was dampened by substances tested. All substances tested were found to have antioxidant properties, as demonstrated by a reduction in ROS production in mesenteric vessels. This effect was confirmed in curcumin and extract of Mentha x villosa which reduced reperfusion dyshythmias.

Dissection of Mechanisms of a Chinese Medicinal Formula: Danhong Injection Therapy for Myocardial Ischemia/Reperfusion InjuryIn VivoandIn Vitro

“…By analyzing the concentration ratios against standard substances, those components with the highest concentration in Salvia miltiorrhiza and Carthamus tinctorius were determined as the potential active ingredients, which were then used for pharmacodynamic screening. Although protocatechuic aldehyde and rosmarinic acid are reported to have cardioprotective effects against I/R injury [26, 27], this was discrepant to our results; the reason may be related to the concentration of these substances, since the administrated dosage in the present study was adjusted according to their respective levels in DHI. Finally, compounds A, B, and C were prioritized for further research following the beneficial results.…”

Rosmarinic Acid Protects against Inflammation and Cardiomyocyte Apoptosis during Myocardial Ischemia/Reperfusion Injury by Activating Peroxisome Proliferator-Activated Receptor Gamma

“…After treatment of rats with these above doses of RosA, the hemodynamic parameters in heart and the infarct size of myocardium were examined, according to the preliminary experiments data, the dose of 10 μM of RosA was used in our next experiments (Supplementary Figures 1, 2), which is in accordance with previous studies that determined that 10 μM of RosA has a cardioprotective effect (Brosková et al, 2013). Previous studies have also suggested that 5 μM of Pioglitazone (PIO), a PPARγ ligand, has a cardioprotective effect (Wynne et al, 2005), therefore, this dose of PIO was used in subsequent experiments.…”

Cardioprotective Effects of Pomegranate (Punica granatum) Juice in Patients with Ischemic Heart Disease

“…Reactive oxygen species (ROS) play a critical role in the pathogenesis of reperfusion‐induced injury. In fact, the use of ROS scavengers has shown beneficial effects in attenuation of oxidative damage (Brosková et al, ). Some potent antioxidants with plant origin have been assayed in this regard.…”