Effect of plant phenolic compounds on biofilm formation by Pseudomonas aeruginosa

Plyuta, V. A.; Zaitseva, Julia N.; Лобакова, Е. С.; Загоскина, Н. В.; Kuznetsov, Alexander; Khmel, I. A.

doi:10.1111/apm.12083

Cited by 75 publications

(67 citation statements)

References 34 publications

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“…Biofilm formation of P. aeruginosa PAO1 was enhanced 3-to 7-fold under the action of epicatechin. Epicatechin caused an up to 5-fold enhancement of the P. aeruginosa PAO1 biofilm formation at sub-inhibitory concentrations up to 750 µg mL -1 (Plyuta et al, 2013). 574',-Trihydroxyfl3avan-3-ol 4 (1 -2.5 mg mL -1 ) and isovitexin 6 (0.2 -0.5 mg mL -1 ) exposures also decreased adhesion of methicillin-susceptible S. aureus ATCC 29213 (Figs.…”

Section: Biofilm Microtitre Plate Assaysmentioning

confidence: 92%

Antibacterial and Anti-Biofilm Activity of Flavonoids and Triterpenes Isolated from The Extracts of Ficus Sansibarica Warb. Subsp. Sansibarica (Moraceae) Extracts

Awolola¹,

Koorbanally²,

Chenia³

et al. 2014

Afr. J. Trad. Compl. Alt. Med.

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Background: Ficus species are used in African traditional medicine in the treatment of a wide variety of ailments and diseases such as convulsive disorder, wound healing, gonorrhea, tuberculosis, diabetes, diarrhoeal infections, dysentery, malaria and HIV. The aim of this study was to isolate the phytochemical constituents in the plant and test them for their antibacterial activity. Materials and methods: The fruits, leaves and stem bark were extracted with organic solvents and the compounds in the extracts separated and purified by column chromatography before being identified by NMR spectroscopy and by comparison of the NMR data against values reported in the literature. The antibacterial activity of the pure compounds and extracts were tested using the disk diffusion method. Results: Three triterpenes and three flavonoids: lupeol acetate (1); cycloart-23-ene-3,25-diol (2); β-sitosterol (3); 5,7,4'-trihydroxyflavan-3-ol (4); epicatechin (5); and isovitexin (6) were isolated in this study. Antimicrobial activity was observed at 8 mg mL -1 for Staphylococcus aureus ATCC 29213 with four of the six isolated compounds, with no activity being observed at 1 -4 mg mL -1 against Escherichia coli ATCC 25922, E. coli ATCC 35218 and S. aureus ATCC 43300. Epicatechin (5) was found to decrease adhesion of E. coli ATCC 25922 and S. aureus ATCC 29213. Decreased adhesion of S. aureus ATCC 29213 was also observed with 5,7,4'-trihydroxyflavan-3-ol (4) and isovitexin (6). Conclusions:The results of this study provide baseline information on F. sansibarica's potential validity in the treatment of infections associated with Gram-positive microorganisms.

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Section: Biofilm Microtitre Plate Assaysmentioning

confidence: 92%

Antibacterial and Anti-Biofilm Activity of Flavonoids and Triterpenes Isolated from The Extracts of Ficus Sansibarica Warb. Subsp. Sansibarica (Moraceae) Extracts

Awolola¹,

Koorbanally²,

Chenia³

et al. 2014

Afr. J. Trad. Compl. Alt. Med.

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“…Bacterial cells in biofilms are extremely resistant to treatment with antibiotics and other drugs as well as to the immune response of the host organism. Biofilms of pathogenic bacteria on medical devices, including catheters or lenses, can cause chronic infections (Bjarnsholt 2013, Plyuta et al 2013. The importance of biofilm is well recognized in medical, environmental, and industrial contexts (Stepanovic et al 2007).…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts

Pinto

Silva

Menegati

et al. 2017

An. Acad. Bras. Ciênc.

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This study aimed to further investigate the cytotoxicity against tumor cell lines and several bacterial strains of Annona squamosa and its mode of action. Methanol extracts of A. squamosa leaves (ASL) and seeds (ASS) were used. ASL showed significant antibacterial activity against S. aureus, K. pneumoniae and E. faecalis with MIC values of 78, 78 and 39 µg/mL respectively. Moreover, ASL exhibited significant biofilm disruption, rapid time dependent kinetics of bacterial killing, increased membrane permeability and significantly reduced the cell numbers and viability. Regarding the cytotoxicity against tumor cell lines, ASS was more active against Jurkat and MCF-7 cells, with CI 50 1.1 and 2.1 µg/mL, respectively. ASL showed promising activity against Jurkat and HL60, with CI 50 4.2 and 6.4 µg/mL, respectively. Both extracts showed lower activity against VERO cells and reduced the clonogenic survival at higher concentrations (IC 90 ) to MCF-7 and HCT-116 lineages. The alkaloids anonaine, asimilobine, corypalmine, liriodenine nornuciferine and reticuline were identified in extracts by UPLC-ESI-MS/MS analysis. This study reinforced that A. squamosa presents a remarkable phytomedicinal potential and revealed that its antimicrobial mechanism of action is related to bacterial membrane destabilization.

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“…Most recently, Plyuta et al . have found phenolic plant extracts that are able to inhibit biofilm formation in the P. aeruginosa PAO1 strain when applied at high concentrations [122]. However, at sub-inhibitory concentrations, these compounds have an opposite effect and supported biofilm growth instead.…”

Section: Quorum Sensing Disruption Strategies In Gram-negative Bacmentioning

confidence: 99%

Development of Quorum-Based Anti-Virulence Therapeutics Targeting Gram-Negative Bacterial Pathogens

Tay

Yew

2013

IJMS

103

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Quorum sensing is a cell density-dependent signaling phenomenon used by bacteria for coordination of population-wide phenotypes, such as expression of virulence genes, antibiotic resistance and biofilm formation. Lately, disruption of bacterial communication has emerged as an anti-virulence strategy with enormous therapeutic potential given the increasing incidences of drug resistance in pathogenic bacteria. The quorum quenching therapeutic approach promises a lower risk of resistance development, since interference with virulence generally does not affect the growth and fitness of the bacteria and, hence, does not exert an associated selection pressure for drug-resistant strains. With better understanding of bacterial communication networks and mechanisms, many quorum quenching methods have been developed against various clinically significant bacterial pathogens. In particular, Gram-negative bacteria are an important group of pathogens, because, collectively, they are responsible for the majority of hospital-acquired infections. Here, we discuss the current understanding of existing quorum sensing mechanisms and present important inhibitory strategies that have been developed against this group of pathogenic bacteria.

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Effect of plant phenolic compounds on biofilm formation by Pseudomonas aeruginosa

Cited by 75 publications

References 34 publications

Antibacterial and Anti-Biofilm Activity of Flavonoids and Triterpenes Isolated from The Extracts of <i>Ficus Sansibarica</i> Warb. <i>Subsp. Sansibarica</i> (Moraceae) Extracts

Antibacterial and Anti-Biofilm Activity of Flavonoids and Triterpenes Isolated from The Extracts of <i>Ficus Sansibarica</i> Warb. <i>Subsp. Sansibarica</i> (Moraceae) Extracts

Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts

Development of Quorum-Based Anti-Virulence Therapeutics Targeting Gram-Negative Bacterial Pathogens

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