Effect of photon fluence rate, nitrogen limitation and nitrogen recovery on the level of phycoerythrin in the unicellular alga, <i>Rhodosorus marinus</i> (Rhodophyceae)

Dupré, Catherine; Guary, J.C.; Grizeau, Dominique

doi:10.1111/j.1399-3054.1994.tb08846.x

Cited by 12 publications

(10 citation statements)

References 17 publications

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“…Rhodosorus marinus Geitler (Stylonemataceae) is a unicellular red microalga belonging to the phylum Rhodophyta, and commonly found in tropical and subtropical seas. To date, Rhodosorus marinus has been mainly exploited as a source of phycobiliproteins, in particular, phycoerythrin, phycocyanin, and allophycocyanin, which are water-soluble light-harvesting pigments [ 15 , 16 , 17 ]. A study conducted in 2001 revealed an inhibitory effect of the ethanol-insoluble fraction of Rhodosorus marinus on hyaluronidase activation, suggesting a potential effect in anti-inflammatory and anti-allergic reactions [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

GABA and GABA-Alanine from the Red Microalgae Rhodosorus marinus Exhibit a Significant Neuro-Soothing Activity through Inhibition of Neuro-Inflammation Mediators and Positive Regulation of TRPV1-Related Skin Sensitization

et al. 2018

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The aim of the present study was to investigate the neuro-soothing activity of a water-soluble hydrolysate obtained from the red microalgae Rhodosorus marinus Geitler (Stylonemataceae). Transcriptomic analysis performed on ≈100 genes related to skin biological functions firstly revealed that the crude Rhodosorus marinus extract was able to significantly negatively modulate specific genes involved in pro-inflammation (interleukin 1α encoding gene, IL1A) and pain detection related to tissue inflammation (nerve growth factor NGF and its receptor NGFR). An in vitro model of normal human keratinocytes was then used to evaluate the ability of the Rhodosorus marinus extract to control the release of neuro-inflammation mediators under phorbol myristate acetate (PMA)-induced inflammatory conditions. The extract incorporated at 1% and 3% significantly inhibited the release of IL-1α and NGF secretion. These results were confirmed in a co-culture system of reconstructed human epithelium and normal human epidermal keratinocytes on which a cream formulated with the Rhodosorus marinus extract at 1% and 3% was topically applied after systemic induction of neuro-inflammation. Finally, an in vitro model of normal human astrocytes was developed for the evaluation of transient receptor potential vanilloid 1 (TRPV1) receptor modulation, mimicking pain sensing related to neuro-inflammation as observed in sensitive skins. Treatment with the Rhodosorus marinus extract at 1% and 3% significantly decreased PMA-mediated TRPV1 over-expression. In parallel with these biological experiments, the crude Rhodosorus marinus extract was fractionated by centrifugal partition chromatography (CPC) and chemically profiled by a recently developed 13C NMR-based dereplication method. The CPC-generated fractions as well as pure metabolites were tested again in vitro in an attempt to identify the biologically active constituents involved in the neuro-soothing activity of the Rhodosorus marinus extract. Two active molecules, namely, γ-aminobutyric acid (GABA) and its structural derivative GABA-alanine, demonstrated a strong capacity to positively regulate skin sensitization mechanisms related to the TRPV1 receptors under PMA-induced inflammatory conditions, therefore providing interesting perspectives for the treatment of sensitive skins, atopia, dermatitis, or psoriasis.

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Section: Introductionmentioning

confidence: 99%

GABA and GABA-Alanine from the Red Microalgae Rhodosorus marinus Exhibit a Significant Neuro-Soothing Activity through Inhibition of Neuro-Inflammation Mediators and Positive Regulation of TRPV1-Related Skin Sensitization

et al. 2018

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“…In the past, R. marinus has proven useful for research in photosynthesis, pigment chemistry (Giraud 1963;Dupré et al 1994) and fine structure (Giraud 1962;Ford 1984;Broadwater and Scott 1994). Karsten et al (1999) showed that the low molecular weight carbohydrates digeneaside and sorbitol are present in Rhodosorus, but not in any other members of the Bangiophyceae.…”

Section: Introductionmentioning

confidence: 99%

Chloroplast rotation and morphological plasticity of the unicellular alga Rhodosorus (Rhodophyta, Stylonematales)

Wilson¹,

West²,

Pickett‐Heaps³

et al. 2002

Phycological Res

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Time-lapse videomicroscopy was used to film chloroplast rotation in the 13 isolates of Rhodosorus marinus Geitler and one isolate of Rhodosorus magnei Fresnel & Billard maintained in culture. Cell diameter, rates of chloroplast rotation and the number of chloroplast lobes were measured in all isolates. Chloroplast rotation is a definitive characteristic of the genus Rhodosorus and should be included in its taxonomic description. Isolates of the type species, R. marinus, measure 4-7 µm in diameter in low light (2-4 µmol photons m -2 s -1 ) and 4.5-11.5 µm in diameter in bright light (15-20 µmol photons m -2 s -1 ), and have two to seven chloroplast lobes. Rhodosorus magnei is 9 µm in diameter and has seven to nine chloroplast lobes in bright light. However, these cells are much smaller (approx. 4 µm diam.) and have only two to three chloroplast lobes when maintained in low light. The species R. magnei was created based on its larger cell size and numerous chloroplast lobes compared with R. marinus, but since these characteristics were found to be quite variable and dependant on culture conditions, they cannot be used to differentiate these two species.

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“…Recently, it was confirmed that phycoerythrin accumulation is inversely proportional to photon fluence rate (Dupre et al, 1994). Recently, it was confirmed that phycoerythrin accumulation is inversely proportional to photon fluence rate (Dupre et al, 1994).…”

Section: Resultsmentioning

confidence: 94%

“…This unicellular Rhodophyceae has been shown to be an autoflocculent species (Dupre et al, 1994). In this paper, we report a study of a red microalga, RhocEosorus marinus.…”

Section: Introductionmentioning

confidence: 95%

Culture of an autoflocculent microalga in a vertical tubular photobioreactor for phycoerythrin production

1995

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A red microalgal, Rhodosorus marinus, was used to produce phycoerythrin. This autoflocculent species, grown in a vertical tubular photoreactor equipped with a gaz-lift system, achieved a growth rate of 0.029 h-1 with a maximum biomass yield of 2 g.l-1 dry weight. These values were close to those obtained in batch cultures of other red microalgae cultured as free cell suspensions. Resuits have shown that these algae could be grown efficiently in natural seawater enriched with nutrients and harvested by decantation followed by filtratic7.

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Effect of photon fluence rate, nitrogen limitation and nitrogen recovery on the level of phycoerythrin in the unicellular alga, Rhodosorus marinus (Rhodophyceae)

Cited by 12 publications

References 17 publications

GABA and GABA-Alanine from the Red Microalgae Rhodosorus marinus Exhibit a Significant Neuro-Soothing Activity through Inhibition of Neuro-Inflammation Mediators and Positive Regulation of TRPV1-Related Skin Sensitization

GABA and GABA-Alanine from the Red Microalgae Rhodosorus marinus Exhibit a Significant Neuro-Soothing Activity through Inhibition of Neuro-Inflammation Mediators and Positive Regulation of TRPV1-Related Skin Sensitization

Chloroplast rotation and morphological plasticity of the unicellular alga Rhodosorus (Rhodophyta, Stylonematales)

Culture of an autoflocculent microalga in a vertical tubular photobioreactor for phycoerythrin production

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