Effect of phenylephrine and endothelium on vasomotion in rat aorta involves potassium uptake

Palacios, Javier; Vega, José Luis; Paredes, Adrián; Cifuentes, Fredi

doi:10.1007/s12576-012-0240-9

Cited by 11 publications

(5 citation statements)

References 35 publications

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“…In a previous study, we had demonstrated that the addition of SNP in the bath of intact aortic rings pre-contracted with PE and preincubated with Nx-nitro-L-arginine (L-NNA; nitric oxide synthase inhibitor) restored the vasomotion [14]. Similar results were obtained in different vascular beds by removing the endothelium and addition of cyclic guanosine monophosphate (cGMP) [15] or by inhibiting eNOS and adding of SNP [30].…”

Section: Discussionsupporting

confidence: 59%

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Synchronization in the Heart Rate and the Vasomotion in Rat Aorta: Effect of Arsenic Trioxide

2015

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Arsenic trioxide (As2O3) is used clinically in the management of acute promyelocytic leukemia, and the use of electrocardiogram (ECG) in this management is important as arsenic use may cause distortion of the electrical properties with its attendant sequel. We studied the effect of As2O3 on vasomotion in rat aortic rings using isometric tension recordings and ECG in anesthetized rats. The results showed that As2O3 (10(-5) M) significantly (p < 0.01) reduced the frequency of acetylcholine (10(-5) M ACh)- and KCl (10 mM)-induced vasomotion, and it also increased the relaxation time (R t) of vasomotion. This effect was restored by 10(-8) M sodium nitroprusside (nitric oxide donor). ACh-induced NO release in the aorta was blunted in the presence of As2O3. The corrected QT interval (QTc) of the ECG, and time dilation (T d) of the pulse wave in the tail artery of the anesthetized rat were significantly (p < 0.05) increased in the arsenic-treated group (50 ppb As) versus control. In conclusion, data suggest that arsenic-induced reduction in vasomotion frequency of the isolated aortic rings is associated with a decreased bioavailability of NO, an increase in QTc and a decrease in the frequency of the pulse wave generated by the cardiac cycle in anesthetized rats.

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Section: Discussionsupporting

confidence: 59%

“…In isolated aortic rings, the vessel wall has rhythmic contractions (vasomotion), which consists of phasic contractions of vascular tone, alternating cycles of contraction and relaxation [14].…”

Section: Introductionmentioning

confidence: 99%

Synchronization in the Heart Rate and the Vasomotion in Rat Aorta: Effect of Arsenic Trioxide

2015

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“…The KCl-induced contraction was caused by an increase of extracellular potassium, leading to membrane depolarization, which increases calcium influx from extracellular sources, involving voltage-dependent calcium channels [ 9 ]. BaCl 2 and TEA block inward rectifying potassium channels [ 10 ], or potassium channels activated by calcium [ 11 ], respectively, thus depolarizing the plasma membrane and vasoconstriction. Similar results were obtained with the inhibition of Na,K-ATPase, which causes depolarization of plasma membrane and induced vasoconstriction [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

“…To evaluate the vascular function of the endothelium, the vasodilation to 10 −5 M acetylcholine (ACh, muscarinic agonist) in pre-contracted aortic rings with 10 −6 M PE was tested. According to the general use of rat aorta as a pharmacological tool for in vitro, the aortic rings were considered with a functional endothelial response if vasodilation was up to 70–80% [ 11 ].…”

Section: Methodsmentioning

confidence: 99%

8-Oxo-9-Dihydromakomakine Isolated from Aristotelia chilensis Induces Vasodilation in Rat Aorta: Role of the Extracellular Calcium Influx

et al. 2018

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8-Oxo-9-dihydromakomakine is a tetracyclic indole alkaloid extracted from leaves of the Chilean tree Aristotelia chilensis. The present study investigated the effects of this alkaloid on vascular response in tissues isolated from aortic segments obtained from normotensive rats. Our results showed that 8-oxo-9-dihydromakomakine induced a dose-dependent relaxation of aortic rings pre-contracted with phenylephrine (PE; 10−6 M). The vasorelaxation induced by 8-oxo-9-dihydromakomakine in rat aortic rings is independent of endothelium. The pre-incubation of aortic rings with 8-oxo-9-dehydromakomakine (10−4 M) significantly reduced the contractile response to KCl (p < 0.001) more than PE (p < 0.05). The highest dose of 8-oxo-9-dehydromakomakine (10−4 M) drastically reduced the contraction to KCl (6·10−2 M), but after that, PE (10−6 M) caused contraction (p < 0.05) in the same aortic rings. The addition of 8-oxo-9-dihydromakomakine (10−5 M) decreased the contractile response to tetraethylammonium (a voltage-dependent potassium channels blocker; TEA; 5 × 10−3 M; p < 0.01) and BaCl2 (a non-selective inward rectifier potassium channel blocker; 5 × 10−3 M; p < 0.001) in rat aorta. 8-oxo-9-dihydromakomakine (10−5 M) decreased the contractile response to PE in rat aorta in the presence or absence of ouabain (an inhibitor of Na,K-ATPase; 10−3 M; p < 0.05). These results could indicate that 8-oxo-9-dihydromakomakine partially reduces plasma membrane depolarization-induced contraction. In aortic rings depolarized by PE, 8-oxo-9-dihydromakomakine inhibited the contraction induced by the influx of extracellular Ca2+ in a Ca2+ free solution (p < 0.01). 8-oxo-9-dihydromakomakine reduced the contractile response to agonists of voltage-dependent calcium channels type L (Bay K6844; 10−8 M; p < 0.01), likely decreasing the influx of extracellular Ca2+ through the voltage-dependent calcium channels. This study provides the first qualitative analysis indicating that traditional folk medicine Aristotelia chilensis may be protective in the treatment of cardiovascular pathologies.

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“…Alternatively, sympathetic vasoconstrictive responses can also be artificially induced and investigated in preclinical models by the administration of exogenous drugs. PE, a selective agonist for α 1 -adrenergic receptors, is generally known to elicit dose-dependent vasocontraction in isolated rat aortae [ 58 , 59 ]. Our group previously demonstrated that chronic exposure to HMW-AGEs significantly strengthens aortic contraction after cumulative doses of PE [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Acute Exposure to Glycated Proteins Impaired in the Endothelium-Dependent Aortic Relaxation: A Matter of Oxidative Stress

D'Haese

Deluyker

Bito

2022

IJMS

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Chronically increased levels of high molecular weight advanced glycation end products (HMW-AGEs) are known to induce cardiovascular dysfunction. Whether an acute increase in HMW-AGE levels affects vascular function remains unknown. In this study, we examined whether acute exposure to HMW-AGEs disturbs aortic vasomotor function. Aortae were obtained from healthy male rats and were acutely pre-treated with HMW-AGEs in organ baths. Aortic relaxation responses to cumulative doses of acetylcholine (ACh), in the presence or absence of superoxide dismutase (SOD), were measured after precontraction with phenylephrine (PE). Furthermore, levels of 3-nitrotyrosine were evaluated on aortic paraffine sections. In our study, we show that acute exposure to HMW-AGEs significantly decreases the aortic relaxation response to ACh. SOD pre-treatment prevents acute HMW-AGEs-induced impairment by limiting superoxide formation. In conclusion, our data demonstrate that acute exposure to HMW-AGEs causes adverse vascular remodelling, characterised by disturbed vasomotor function due to increased oxidative stress. These results create opportunities for future research regarding the acute role of HMW-AGEs in cardiovascular dysfunction.

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Effect of phenylephrine and endothelium on vasomotion in rat aorta involves potassium uptake

Cited by 11 publications

References 35 publications

Synchronization in the Heart Rate and the Vasomotion in Rat Aorta: Effect of Arsenic Trioxide

Synchronization in the Heart Rate and the Vasomotion in Rat Aorta: Effect of Arsenic Trioxide

8-Oxo-9-Dihydromakomakine Isolated from Aristotelia chilensis Induces Vasodilation in Rat Aorta: Role of the Extracellular Calcium Influx

Acute Exposure to Glycated Proteins Impaired in the Endothelium-Dependent Aortic Relaxation: A Matter of Oxidative Stress

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