Effect of phase system selectivity in preparative column liquid chromatography

Wehrli, A.; Hermann, U.; Huber, J.F.K.

doi:10.1016/s0021-9673(00)93811-1

Cited by 39 publications

(3 citation statements)

References 5 publications

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“…Micro-scale liquid chromatography is performed in columns of PTFE tubing whose volume is less than 0.01 cm3 (201). At the other end of the scale, attention is being paid to preparative chromatography (393,498) and its automatic control (50).…”

Section: Liquid Chromatographymentioning

confidence: 99%

Ion exchange and liquid column chromatography

Walton¹

1978

Anal. Chem.

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Section: Liquid Chromatographymentioning

confidence: 99%

Ion exchange and liquid column chromatography

Walton¹

1978

Anal. Chem.

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“…Moreover, as the distance between peaks is related to both purity and recovery in preparative chromatography, it was demonstrated that the amount of recovered molecules was significantly higher in 2D separations compared to a succession of 1D separations 1 . The first 2D preparative separation was realized in 1976 in order to recover the maximum amount of two compounds in a simple mixture 2 . This approach led to the development of 2D preparative separations with both the use of liquid chromatography (LC) and liquid-liquid chromatography (LLC).…”

mentioning

confidence: 99%

“…1 The first 2D preparative separation was realized in 1976 to recover the maximum amount of two compounds in a simple mixture. 2 This approach led to the development of 2D preparative separations with both the use of liquid chromatography (LC) and liquid−liquid chromatography (LLC). As described in a recent review from our group, 3 the first 2D preparative separations involving LLC were dedicated to the isolation of natural products using countercurrent chromatography (CCC) technology in heart-cut mode.…”

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confidence: 99%

In Silico Screening of Comprehensive Two-Dimensional Centrifugal Partition Chromatography × Liquid Chromatography for Multiple Compound Isolation

et al. 2018

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The isolation of unknown compounds for structural identification and the collection of target molecules to generate unavailable standards remain a challenge when dealing with complex sample. While tedious multistep purification is commonly used, it is not appropriate for a limited amount of sample or when a full recovery of expensive molecules is required. Two-dimensional preparative chromatography in a comprehensive mode provides an effective mean to collect a large number of molecules in such a case. However, there is currently a lack of metrics to estimate preparative performances with a minimal number of experiments. An in silico comparative study of various pairs of chromatographic systems is proposed, focusing on the occupation rate and the homogeneity of peak spreading in the 2D separation space. Offline combination of centrifugal partition chromatography (CPC) with liquid chromatography (LC) exhibits numerous advantages for 2D preparative separation. Our in silico approach was illustrated through the isolation of eight bioactives compounds with very similar structures from Cyclopia genistoides plant by CPCxLC. The column screening was performed considering predictive 2D plots in the light of the preparative performance descriptors and compared to real 2D preparative separations.

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Evaluation of loop and pump injection systems in preparative liquid chromatography

Gazda

Kowalczyk

1983

J. High Resol. Chromatogr.

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SummaryThe usefulness of pump or loop injectors in preparative liquid chromatography was evaluated. The concentration distribution of samples flowing through a UV detector cell, directly connected to theinjector, was recorded at variousflowvelocities, at various diameters of loop tubing, and using various methods of injection [injection with sample volumes equal to a fraction or total loop volume, etc.]. The most advantageous methods were found to be either use of a loop injector to inject only a fraction of itstotalvolumeor useofapump. Bothofthesemethodsensurea almost rectangular concentration distribution.

show abstract

Effect of phase system selectivity in preparative column liquid chromatography

Cited by 39 publications

References 5 publications

Ion exchange and liquid column chromatography

Ion exchange and liquid column chromatography

In Silico Screening of Comprehensive Two-Dimensional Centrifugal Partition Chromatography × Liquid Chromatography for Multiple Compound Isolation

Evaluation of loop and pump injection systems in preparative liquid chromatography

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