Effect of phagocytosis on the reduced soluble sulfhydryl content of human granulocytes

Ds, Mendelson; Metz, Earl N.; Sagone, AL Jr

doi:10.1182/blood.v50.6.1023.bloodjournal5061023

Cited by 3 publications

(3 citation statements)

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“…Whole blood was incubated at 37°C for 1 h in 0.1 volume of 4% dextran. The plasma, containing leukocytes and platelets, was removed, mixed in Seligman balanced salt solution, and layered over a standard Ficoll-Hypaque mixture to remove platelets and mononuclear cells (11,13). This mixture was centrifuged for 35 min at 800 x g and then aspirated down to the bottom which contained granulocytes and a few erythrocytes Hypotonic-shock lysis eliminated the remaining erythrocytes.…”

Section: Methodsmentioning

confidence: 99%

Extracellular metabolism of thyroid hormones by stimulated granulocytes

Rao¹,

Sagone²

1984

Infect Immun

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Stimulated polymorphonuclear leukocytes have been shown by this laboratory to release a reactiveoxygen species (ROS) which is detectable in the supernatant and is capable of oxidizing reduced glutathione and reacting with methionine (Sagone et al., Blood 63:96-104, 1984). This ROS is dependent on H2O0 and heme for its production and is postulated to be a stable oxidant derived from hypochlorous acid, such as a chloramine. Further, this ROS was also shown to be able to oxidize and fix iodide to protein. This latter characteristic was the theoretical basis for our present study in which the same ROS was shown to be able to carry out the iodination of 3,3,5'-triiodothyronine to thyroxine in the presence of I-. Our results provide further support that granulocytes have a role in the peripheral utilization of thyroid hormones in patients with infectious diseases or other illnesses in which granulocytes may be activated, and our results indicate that the reactions may occur extracellularly.

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Section: Methodsmentioning

confidence: 99%

Extracellular metabolism of thyroid hormones by stimulated granulocytes

Rao¹,

Sagone²

1984

Infect Immun

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“…Starting from the assumption that ELF GSH is derived from epithelial cells, the authors hypothesized that the chronic oxidant burden in IPF causes an alteration in their ability to synthesize, store and secrete GSH. In this regard, studies in neutrophils and lung tissue have shown that OS reduces the intracellular concentrations of both GSH and GSSG [ 47 , 48 ], and that oxidative stress in general may affect GSH transport in epithelial cells.…”

Section: Biomarkers Of Oxidative Stress In Ipfmentioning

confidence: 99%

Evaluation of oxidative stress biomarkers in idiopathic pulmonary fibrosis and therapeutic applications: a systematic review

et al. 2018

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IntroductionIdiopathic pulmonary fibrosis (IPF), a fatal lung disease of unknown origin, is characterized by chronic and progressive fibrosing interstitial pneumonia which progressively impairs lung function. Oxidative stress is one of the main pathogenic pathways in IPF. The aim of this systematic review was to describe the type of markers of oxidative stress identified in different biological specimens and the effects of antioxidant therapies in patients with IPF.MethodsWe conducted a systematic search of publications listed in electronic databases (Pubmed, Web of Science, Scopus and Google Scholar) from inception to October 2017. Two investigators independently reviewed all identified articles to determine eligibility.ResultsAfter a substantial proportion of the initially identified articles (n = 554) was excluded because they were duplicates, abstracts, irrelevant, or did not meet the selection criteria, we identified 30 studies. In each study, we critically appraised the type, site (systemic vs. local, e.g. breath, sputum, expired breath condensate, epithelial lining fluid, bronchoalveolar lavage, and lung tissue specimens), and method used for measuring the identified oxidative stress biomarkers. Furthermore, the current knowledge on antioxidant therapies in IPF was summarized.ConclusionsA number of markers of oxidative stress, with individual advantages and limitations, have been described in patients with IPF. Nevertheless, trials of antioxidant treatments have been unable to demonstrate consistent benefits, barring recent pharmacogenomics data suggesting different results in specific genotype subgroups of patients with IPF.

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“…Monocytes and neutrophils were also isolated by these methods from patients with CGD. The diagnosis was made using criteria previously reported from our laboratory (24).…”

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confidence: 99%

Activation of Monocyte and Granulocyte Antibody-Dependent Cytotoxicity by Phorbol Myristate Acetate

1982

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We have characterized the effects of phorbol myristate acetate (PMA) on human monocyte and neutrophil oxidative metabolism and antibody-dependent cytotoxicity toward anti-D sensitized human erythrocytes (RBC) and a human lymphoblastoid cell line (CEM). Hexose monophosphate shunt activity was measured by [1- 14 C]glucose oxidation and target lysis by 51 Cr release. PMA produced a dose-dependent stimulation of hexose monophosphate shunt activity. Neutrophils responded with higher hexose monophosphate shunt activity and at a lower PMA concentration than did monocytes. PMA increased monocyte lysis of antibody-sensitized RBC by two-thirds, but did not affect lysis of CEM targets. Neutrophils were unable to lyse either antibody-sensitized or nonsensitized RBC without the addition of PMA. When PMA was added, lysis of both targets increased markedly. Neutrophils without PMA were able to lyse a small number of both antibody-sensitized and nonsensitized CEM targets. PMA also increased neutrophil lysis of these targets. Target lysis by neutrophils from a patient with chronic granulomatous disease, cells unable to produce reactive oxygen species, was not increased by PMA. Chronic granulomatous disease monocytes, however, responded to PMA by more than doubling lysis of antibody-sensitized RBC. Hypoxia inhibited PMA augmentation of antibody-sensitized RBC lysis by neutrophils, but not by monocytes. Generation of reactive oxygen species by the xanthine-xanthine oxidase system inhibited CEM growth, but did not cause lysis, indicating that in some cases oxidative injury may be nonlytic. We suggest that PMA augments neutrophil cytotoxicity to tumor and RBC targets by stimulating reactive oxygen species-mediated lysis, but in monocytes augmentation of lysis is due to activation of a nonoxidative mechanism of lysis.

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Effect of phagocytosis on the reduced soluble sulfhydryl content of human granulocytes

Cited by 3 publications

References 0 publications

Extracellular metabolism of thyroid hormones by stimulated granulocytes

Extracellular metabolism of thyroid hormones by stimulated granulocytes

Evaluation of oxidative stress biomarkers in idiopathic pulmonary fibrosis and therapeutic applications: a systematic review

Activation of Monocyte and Granulocyte Antibody-Dependent Cytotoxicity by Phorbol Myristate Acetate

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