Effect of pH on the isolation of urinary exosome

Zhao, Yao; Chen, Kaiyong; Li, Haining; Wu, Han

doi:10.1007/s11255-016-1408-7

Cited by 26 publications

(20 citation statements)

References 24 publications

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“…These different methods may vary in purity of the resulting EVs and in the complexity of the protocol, and therefore in their possible clinical application. To avoid the analysis of urinary contaminants, such as shed cells or unbound proteins, some technical precautions are needed including centrifugations or filtration steps, addition of protease inhibitors, and pH control (Zhou et al, 2006a; Zhao et al, 2017). The advantage of EVs as biomarker is the possibility to obtain different sets of information.…”

Section: Evs As Renal Disease Biomarkersmentioning

confidence: 99%

Extracellular Vesicles in Renal Pathophysiology

Pomatto

Gai

Bussolati

et al. 2017

Front. Mol. Biosci.

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Extracellular vesicles are a heterogeneous population of microparticles released by virtually all living cells which have been recently widely investigated in different biological fields. They are typically composed of two primary types (exosomes and microvesicles) and are recently commanding increasing attention as mediators of cellular signaling. Indeed, these vesicles can affect recipient cells by carrying and delivering complex cargos of biomolecules (including proteins, lipids and nucleic acids), protected from enzymatic degradation in the environment. Their importance has been demonstrated in the pathophysiology of several organs, in particular in kidney, where different cell types secrete extracellular vesicles that mediate their communication with downstream urinary tract cells. Over the past few years, evidence has been shown that vesicles participate in kidney development and normal physiology. Moreover, EVs are widely demonstrated to be implicated in cellular signaling during renal regenerative and pathological processes. Although many EV mechanisms are still poorly understood, in particular in kidney, the discovery of their role could help to shed light on renal biological processes which are so far elusive. Lastly, extracellular vesicles secreted by renal cells gather in urine, thus becoming a great resource for disease or recovery markers and a promising non-invasive diagnostic instrument for renal disease. In the present review, we discuss the most recent findings on the role of extracellular vesicles in renal physiopathology and their potential implication in diagnosis and therapy.

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Section: Evs As Renal Disease Biomarkersmentioning

confidence: 99%

Extracellular Vesicles in Renal Pathophysiology

Pomatto

Gai

Bussolati

et al. 2017

Front. Mol. Biosci.

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“…However, in this study no information about changes in the particle concentration was reported. Some other studies revealed the effect of pH, storage temperature and cycles of freezing and thawing only on the yield of exosome isolation, but not on quantity changes during storage (Akers et al, 2016;Ban et al, 2015;Zhao et al, 2017). Therefore, the standard criterion of exosomal preservation condition is still undefined.…”

mentioning

confidence: 99%

Effect of pH, temperature and freezing-thawing on quantity changes and cellular uptake of exosomes

et al. 2018

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“…Typically, exosomes are secreted by all cells with a diameter of 30-120nm lipid bilayer membrane and they can be identified in biological fluids including urine, blood, serum and saliva 13 - 17 . Exosomes are small extracellular vesicles with various molecular constituents including proteins, mRNA and microRNA which have the potential to be biomarkers in various malignancies including PCa 18 .…”

Section: Discussionmentioning

confidence: 99%

Exosomal ephrinA2 derived from serum as a potential biomarker for prostate cancer

Li¹,

Zhao²,

Chen³

et al. 2018

J. Cancer

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Up-regulation of serum ephrinA2 is common in various malignancies and has been suggested as a potential biomarker for the diagnosis and prognosis of prostate cancer (PCa). However, the type of serum ephrinA2 expressed in PCa patients remains elusive. Furthermore, the level of exosomal ephrinA2 derived from serum is increased in patients with osteoporosis, a common complication of PCa patients undergoing androgen deprivation therapy. It is unknown whether exosomes derived from PCa patient serum contains ephrinA2. In this study, we explored the ephrinA2 expression in whole serum and tissues and identified the circulating exosomal ephrinA2 as a potential biomarker for PCa. Exosomes were isolated from patient sera by differential centrifugation and the presence of ephrinA2 was confirmed via electron microscopy and western blotting. The type of ephrinA2 in serum was evaluated by western blotting. The expression of serum ephrinA2 including secreted and cleaved ephrinA2 and exosomal ephrinA2 were detected by ELISA and western blotting. Compared with benign prostatic hyperplasia (BPH) and controls, the levels of whole serum ephrinA2 and exosomal ephrinA2 were significantly higher in PCa patients. Moreover, exosomal ephrinA2 expression was positively correlated with TNM staging and Gleason score of PCa patients. The diagnostic efficiency of exosomal ephrinA2 was superior to that of whole serum ephrinA2 and serum PSA in distinguishing PCa patients from those from BPH patents. Our study indicates that exosomal ephrinA2 has high potential as a biomarker for the presence of PCa and offers a new therapeutic target for this disease.

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Effect of pH on the isolation of urinary exosome

Abstract: Acidic environment was likely to conducive to the isolation of exosome and maintain its stability and integrity that suggest pH medium needs to be carefully considered and also provide a methodology for future separation exosome.

Cited by 26 publications

References 24 publications

Extracellular Vesicles in Renal Pathophysiology

Extracellular Vesicles in Renal Pathophysiology

Effect of pH, temperature and freezing-thawing on quantity changes and cellular uptake of exosomes

Exosomal ephrinA2 derived from serum as a potential biomarker for prostate cancer

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