Effect of pH on red blood cell deformability

Kuzman, Drago; Žnidarčič, T.; Gros, Marjan; Vrhovec, S; Svetina, S.; Žekš, B.

doi:10.1007/s004240000061

Cited by 50 publications

(32 citation statements)

References 6 publications

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“…The last supposition that could explain the trend of erythrocyte deformability in OSAS might be identified in the variation of pH that seems to accompany this syndrome. During the cycles of hypoxia and reoxygenation a condition of respiratory acidosis may also be present and may influence the red cell rheology; there are many findings regarding the effect of the pH on elastic deformability of the erythrocyte membrane [4,16], although in our group of OSAS subjects we have no information about the acid-base balance.…”

Section: Discussionmentioning

confidence: 91%

Erythrocyte deformability, plasma lipid peroxidation and plasma protein oxidation in a group of OSAS subjects

Caimi

Montana

Canino

et al. 2016

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Abstract. Considering that obstructive sleep apnea syndrome (OSAS) is usually associated with endothelial dysfunction, atherosclerosis and cardiovascular disorders, our aim was to examine the erythrocyte deformability and the oxidative status in a group of OSAS subjects. We consecutively enrolled 48 subjects with OSAS defined after a 1-night cardiorespiratory sleep study, subsequently subdivided according to the apnea/hypopnea index (AHI) value in two subgroups: Low (L = 21 subjects with AHI<30) and High (H = 27 subjects with AHI>30). We evaluated the erythrocyte deformability, expressed as elongation index (EI) and the parameters of the oxidative status, such as lipid peroxidation (expressed as thiobarbituric acid-reactive substances -TBARS) and protein oxidation (measured as carbonyl groups -PC). In the entire group and in the two subgroups of OSAS subjects we found a decreased erytrocyte deformability at all shear stresses, not correlated with the plasmatic oxidative stress nor with the polysomnographic parameters. Lipid peroxidation was increased in the whole group and in the H subgroup of OSAS while protein oxidation showed a different trend. As in OSAS the osmotic fragility and the metabolism of the red cells seem to be not impaired, the oxidative damage to the red cell membrane proteins might be responsible for the reduced erythrocyte deformability. This rheological alteration, in addition to the increase in whole blood and plasma viscosity and to the erythrocyte hyperaggregation, could influence the microcircolatory profile in OSAS subjects.

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Section: Discussionmentioning

confidence: 91%

Erythrocyte deformability, plasma lipid peroxidation and plasma protein oxidation in a group of OSAS subjects

Caimi

Montana

Canino

et al. 2016

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“…It is important to examine these control cases so that the other cases, that are RBCs mixed with tumor cells, can prove that the influence on RBCs deformability is extremely limited to the contact of tumor cells. It is reported that an increase of RBC rigidity is caused by lowering pH, elevating temperature, increasing extracellular glucose concentration, or making the suspending medium hypo-or hypertonic [26][27][28]. Moreover, there are studies that cancer cells are more acidic than normal cells and generate acidic or low pH microenvironment which is ideal for invasion and metastasis [29,30].…”

Section: Resultsmentioning

confidence: 99%

Extensional flow-based microfluidic device: deformability assessment of red blood cells in contact with tumor cells

et al. 2014

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Red blood cell (RBC) deformability has become one of the important factors to assess blood and cardiovascular diseases. The interest on blood studies have promoted a development of various microfluidic devices that treat and analyse blood cells. Recent years, besides the RBC deformability assessment, these devices are often applied to cancer cell detection and isolation from the whole blood. The devices for cancer cell isolation rely mainly on size and deformability of the cells. However, the examination of deformability of the RBCs mixed with cancer cells is lacking. This study aims at determining the deformation index (DI) of the RBCs in contact with cancer cells using a hyperbolic microchannel which generates a strong extensional flow. The DIs of human healthy RBCs and human RBCs in contact with a tumor cell line (HCT-15, colon carcinoma) were compared by analyzing the flowing RBCs images captured by a high speed camera. The results reveal that the RBCs that were in contact with HCT-15 cells have lower deformability than the normal RBCs.

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“…The extra effect on the acid-base balance of the body, for example, by the use of potassium hydroxide to dissolve the allopurinol, had to be excluded, as it can disturb the correct value of the erythrocyte deformability. Changes in pH cause alteration in red blood cell rigidity and deformability [31] (the fluid pH after dissolving was 6.9). Allopurinol was administered into the intraperitoneal cavity 30 min before the ischemic insult.…”

Section: Alterations In Erythrocyte Deformability: the Effect Of Allomentioning

confidence: 99%

Allopurinol Prevents Erythrocyte Deformability Impairing but Not the Hematological Alterations After Limb Ischemia–Reperfusion in Rats

Németh

Lesznyák

Szokoly³

et al. 2006

Journal of Investigative Surgery

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The measurement of red blood cell deformability provides a possible method for detecting the effect of ischemia-reperfusion on erythrocytes. In our study the effect of 1-h ischemia-reperfusion with or without allopurinol pretreatment on hematological parameters and red blood cell deformability was investigated in a follow-up experiment of 26 male CD outbred rats that were subjected to unilateral hind-limb ischemia by microvascular clips on femoral vessels for 1 h (IR, n = 6), some rats received allopurinol pretreatment under the same conditions (50 mg/kg, AP + IR, n = 8), others were subjected to sham operation (n = 6), and the rest of animals served as control (n = 6). Measurement of erythrocyte deformability using a bulk filtrometer with special setting of cell suspension hematocrit (1%), and determination of hematological parameters were performed daily for one week. In the IR group, relative cell transit time increased significantly on postoperative days 1 and 2, which was not observed in the other groups. Settings for the measurement of erythrocyte deformability by reducing the blood sample volume gave the possibility of monitoring the resulting changes in rats. Mean corpuscular volume and hemoglobin, platelet count, and platelet volume were higher in the IR and AP + IR groups than in the other groups. In summary, short-term ischemia and reperfusion induced lower red blood cell deformability in the early postoperative period, which could be prevented by allopurinol pretreatment.

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Effect of pH on red blood cell deformability

Cited by 50 publications

References 6 publications

Erythrocyte deformability, plasma lipid peroxidation and plasma protein oxidation in a group of OSAS subjects

Erythrocyte deformability, plasma lipid peroxidation and plasma protein oxidation in a group of OSAS subjects

Extensional flow-based microfluidic device: deformability assessment of red blood cells in contact with tumor cells

Allopurinol Prevents Erythrocyte Deformability Impairing but Not the Hematological Alterations After Limb Ischemia–Reperfusion in Rats

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