Allopurinol Prevents Erythrocyte Deformability Impairing but Not the Hematological Alterations After Limb Ischemia–Reperfusion in Rats

Németh, Norbert; Lesznyák, Tamás; Szokoly, Miklós; Furka, I.; Miko, Iren

doi:10.1080/08941930500444511

Cited by 20 publications

(27 citation statements)

References 25 publications

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“…Nemeth N et al found higher MCV levels for one week measured daily in early postoperative period after unilateral 1 h hind-limb IR on femoral vessels (+allopurinol) than in sham operated and control groups in male rats [6]. Berra HH et al reported enhanced MCV levels after 14 days in experimentally infected rats with Trypanosoma cruzi (Chagas' disease) in comparison with control animals [7].…”

Section: Discussionmentioning

confidence: 99%

The acute effect of erythropoietin on mean corpuscular volume levels during hypoxia-reoxygenation injury in rats

Tsompos¹,

Panoulis

Toutouzas³

et al. 2016

JCB

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Abstract. OBJECTIVE:This experimental study examined the effect of erythropoietin (epo) in a rat model and particularly in a hypoxia-reoxygenation (HR) protocol. The effect of epo was studied hematologically using the blood mean corpuscular volume (MCV) levels. MATERIALS AND METHODS: 40 rats of mean weight 247.7±34.991 g were used in the study. MCV levels were measured at 60 min (groups A and C) and at 120 min (groups B and D) of reoxygenation. Epo was administered only in groups C and D. RESULTS: Epo administration increased non-significantly the predicted MCV levels by 0.56% ± 0.66% (p = 0.3549). The reperfusion time decreased non-significantly the predicted MCV levels by 0.55% ± 0.65% (p = 0.3721). Epo administration and reperfusion time together did also not induce an increase of the MCV levels (0.30% ± 0.39%; p = 0.4430). CONCLUSIONS: Epo administration, reperfusion time and their interaction had no short -term effect on MCV within the time of 2 hours.

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Section: Discussionmentioning

confidence: 99%

The acute effect of erythropoietin on mean corpuscular volume levels during hypoxia-reoxygenation injury in rats

Tsompos¹,

Panoulis

Toutouzas³

et al. 2016

JCB

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“…Decreased erythrocyte deformability leads to microcirculatory disarrangements such as capillary occlusion and diminished oxygen delivery for the whole body (13). In this manner, the examination of erythrocyte deformability may ensure important information on circulation and free radicals during I/R injury (14).…”

Section: Discussionmentioning

confidence: 99%

Effects of intravenous ibuprofen and lornoxicam on erythrocyte deformability in rats undergoing hind limb ischemia reperfusion injury

Şıvgın¹,

Küçük²,

Çomu³

et al. 2017

BLL

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BACKGROUND AND AIM: Acute hind limb ischemia reperfusion (I/R) injury is a common consequence of abdominal aorta cross-clamping during aortic surgery. Erythrocyte deformability is affected by I/R process and may lead to increased tissue and organ injury. Lornoxicam and intravenous ibuprofen are becoming commonly used as non-steroidal anti-infl ammatory drugs (NSAID) for postoperative analgesia. In this study, we aimed to investigate the effects of lornoxicam (2 mg/kg iv) and intravenous ibuprofen (30 mg/kg iv) on erythrocyte deformability in I/R model in rats. MATERIALS AND METHODS: Four study groups, each containing 6 Wistar rats were created. Laparotomy was performed in all groups under general anesthesia with ketamine and xylazine. In all groups except sham group, ischemia and reperfusion were achieved by clamping and declamping the infrarenal abdominal aorta for 120 minutes. Rats in Group IR+L received intravenous infusion of lornoxicam (2 mg/kg) while rats in Group IR+I received intravenous infusion of ibubrofen (30 mg/kg) following 2 hours of ischemic period. At the end of reperfusion period, erythrocyte packs were prepared from heparinized blood samples. Erythrocyte suspensions with hematocrit at a concentration of 5% in a phosphate-buffered saline (PBS) were used in order to perform deformability measurements. The value of p < 0.05 was considered statistically signifi cant. RESULTS: Relative resistance has increased in ischemia reperfusion group when compared to control group (p < 0.0001). Lornoxicam or ibuprofen intravenous treatments did not change the erythrocyte deformability during ischemia reperfusion period in rats (p = 0.851, p = 0.690). CONCLUSION: Intravenous ibuprofen or lornoxicam administrations during ischemia reperfusion period in rats have no negative effect on erythrocyte deformability. The fi ndings of the study should be supported with more detailed and extensive clinical/experimental studies in the future (Fig. 1, Ref. 18). Text in PDF www.elis.sk.

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“…[17][18][19][20][21][22] Besides systemic changes, alterations of properties of local blood in excluded ischemic region and the out-flowing blood from the reperfused region raise more questions to be answered. 14,16,19,20,23 The questions of time factor and circulation-microcirculation of the flaps are in strong relation with the changes of blood flow properties, with hemorheological parameters. These include blood viscosity, plasma viscosity, red blood cell deformability, and red blood cell aggregation, as the most widely investigated parameters in various pathophysiological processes.…”

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confidence: 98%

Hemorheological, morphological, and oxidative changes during ischemia‐reperfusion of latissimus dorsi muscle flaps in a canine model

Tamás¹,

Németh

Bráth

et al. 2009

Microsurgery

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Although ischemia-reperfusion (I/R) strongly influences muscle flap survival in reconstructive surgery, there is limited knowledge about its relation to hemorheological parameters and oxidative stress markers in flaps. In the present study we investigated these changes during I/R of latissimus dorsi muscle (LDM) flaps in beagle dogs. In four animals LDM flaps were prepared bilaterally. The right side served as control, while the left side's vascular pedicle was clamped for 60 minutes, and a 60-minute reperfusion was allowed afterward. Blood samples (0.5 ml each) were taken from the pedicle's vein bilaterally before and after the ischemia, and at the 5th, 15th, 30th, 45th, and 60th minutes of the reperfusion, for hematological and erythrocyte aggregation tests. In muscle biopsies, taken before and after I/R, histological investigations and tests for measuring gluthation-peroxidase (GSH-PX) activity, glutathione (GSH) and carbonyl concentrations, and thiobarbituric acid reactive substances (TBARS) content were carried out. In I/R side leukocyte count increased during the reperfusion with a peak at the 30th minute. Hematocrit continuously increased from the 15th minute. In the first 5 minutes of the reperfusion, erythrocyte aggregation increased, than tented to be normalized. In muscle homogenates GSH-PX activity did not change markedly, GSH content slightly decreased, carbonyl and TBARS content increased during reperfusion. A 1-hour ischemia and reperfusion of LDM flaps caused local changes of leukocyte distribution and erythrocyte aggregation, supposedly due to the metabolic and inflammatory reactions. Oxidative damage during reperfusion was also demonstrated.

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Allopurinol Prevents Erythrocyte Deformability Impairing but Not the Hematological Alterations After Limb Ischemia–Reperfusion in Rats

Cited by 20 publications

References 25 publications

The acute effect of erythropoietin on mean corpuscular volume levels during hypoxia-reoxygenation injury in rats

The acute effect of erythropoietin on mean corpuscular volume levels during hypoxia-reoxygenation injury in rats

Effects of intravenous ibuprofen and lornoxicam on erythrocyte deformability in rats undergoing hind limb ischemia reperfusion injury

Hemorheological, morphological, and oxidative changes during ischemia‐reperfusion of latissimus dorsi muscle flaps in a canine model

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