Effect of pH on biodiesel production and the microbial structure of glucose-fed activated sludge

Sun, Qu; Li, Anjie; Li, Meixi; Hou, Baolian

doi:10.1016/j.ibiod.2015.06.003

Cited by 31 publications

(12 citation statements)

References 22 publications

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“…Raw fastq files were demultiplexed and quality-filtered with QIIME (version 1.17) [ 57 , 58 ]. OTUs (97% similarity) were clustered with UPARSE version 7.1 http://drive5.com/uparse/ ) and chimeric sequences were authenticated and deleted by UCHIME.…”

Section: Methodsmentioning

confidence: 99%

Polysaccharides from Chrysanthemum morifolium Ramat ameliorate colitis rats by modulating the intestinal microbiota community

et al. 2017

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The gut microflora dysbiosis has been closely related with the inflammatory bowel disease (IBD). In this study, the effect of polysaccharides from Chrysanthemum morifolium Ramat on the gut microbiota was evaluated by ulcerative colitis (UC) rat model. Physiological and pathological analyses suggested that Chrysanthemum polysaccharides possessed notably protective effects on UC in vivo. Based on the Illumina MiSeq platform, 16S rRNA sequencing of the rat colonic contents indicated that the intestinal flora structure remarkably changed in the model rats and the tendency was alleviated to a certain degree by treatment with different dosages of Chrysanthemum polysaccharides. In normal groups, there were more Firmicutes than Bacteroidetes, but this change lost at the pathological state. Following Chrysanthemum polysaccharides, rising Firmicutes/Bacteroidetes ratio was validated. Besides the microbial diversity and the community richness of the UC rats were improved by Chrysanthemum polysaccharides, the composition of intestinal microflora in the model group were also restored after oral administration of Chrysanthemum polysaccharides. The abundance of opportunistic pathogens was decreased (Escherichia, Enterococcus and Prevotella), while the levels of protective bacteria such as Butyricicoccus and Clostridium (butyrate-producing bacteria), Lactobacillus and Bifidobacterium (probiotics), Lachnospiraceae and Rikenellaceae elevated in various degrees. Correlation analysis between intestinal flora and biochemical factors suggested that the relative abundance of protective bacteria was positively correlated with the levels of anti-inflammatory cytokines such as IL-4, IL-10 and IL-11, while aggressive bacteria were positively correlated with proinflammatory cytokine such as IL-23、IL-6、 IF-17、TNF-α、IL-1β and IFN-γ. The above results showed that the intestinal flora were closely related to the secretion and expression of cytokines in the body, and they interacted with each other to regulate immune function. Thus, Chrysanthemum polysaccharides could ameliorate ulcerative colitis by fostering beneficial intestinal flora growth, modulating the balance of intestinal microecology and restoring the immune system.

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Section: Methodsmentioning

confidence: 99%

Polysaccharides from Chrysanthemum morifolium Ramat ameliorate colitis rats by modulating the intestinal microbiota community

et al. 2017

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“…Three criteria were followed for demultiplexing and quality-filtering the raw fastq files by QIIME (version 1.17) [54, 55]. Operational taxonomic unit (OTU)-based method was performed to analyze where sequences were split into bins on the basis oftaxonomy and clustered to each bin with the cutoff point of 0.05.…”

Section: Methodsmentioning

confidence: 99%

Deciphering bacterial community changes in zucker diabetic fatty rats based on 16S rRNA gene sequences analysis

Yang

Xiang

et al. 2016

Oncotarget

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The aim of the present pilot study was deciphering bacterial community changes in Zucker diabetic fatty rats (ZDF rats), a model of type 2 diabetes. Recent studies unmasked that the status of gastrointestinal tract microbiota has a marked impact on nutrition-related syndromes such as obesity and type-2 diabetes (T2D). In this study, samples taken from the gastrointestinal tracts (GI tracts) of ZDF and their lean littermates (ZL rats) were subjected to 16S rRNA gene sequence-based analysis to examine the characteristic bacterial communities, including those located in the stomach, duodenum, jejunum, ileum, cecum and feces. Results revealed that the Firmicutes/Bacteroidetes ratio was increased and greater numbers of Lactobacillus were detected along GI tracts in ZDF rats compared to ZL rats. In conclusion, this work is the first study to systematically characterize bacterial communities along ZDF rat GI tract and provides substantial evidence supporting a prospective strategy to alter the GI microbial communities improving obesity and T2D.

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“…The raw Fastq data were processed and analysed as described by Sun et al (2015). Operational Taxonomic Units (OTUs) were clustered with a 97% similarity cutoff from the clean Fastq data and chimeric sequences were identified and removed using Usearch 7.1 (http:// drive5.com/uparse/).…”

Section: Bioinformatics Analysismentioning

confidence: 99%

Response of rumen bacterial diversity and fermentation parameters in beef cattle to diets containing supplemental daidzein

Zhao

Zhou

Bao

et al. 2017

Italian Journal of Animal Science

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An experiment was conducted to determine the effects of soy isoflavone daidzein on ruminal fermentation, microbial protein synthesis and rumen bacterial community in beef cattle. Six rumen-cannulated adult beef cattle were assigned to three blocks according to similar body weight in a randomised block design, with two cattle each block. Each block randomly received one of the two dietary treatments: control (basal concentrate) and daidzein (supplementation with 500 mg daidzein/kg basal concentrate). High-throughput sequencing data showed that supplemental daidzein increased the relative abundance of bacteria belonging to phylum Bacteroidetes (p ¼ .050), but reduced and tended to reduce the relative abundance of phylum Spirochaetae (p ¼ .030) and Firmicutes (p ¼ .080). At the genus level, the relative abundance of Prevotella (p ¼ .036), RC9_gut_group (p ¼ .019), Succinivibrio (p ¼ .093) and Ruminobacter (p ¼ .085) were increased or tended to be increased by daidzein supplementation. However, supplemental daidzein reduced or tended to reduce some uncultured and unclassified bacteria genus belonging to Ruminococcaceae (p ¼ .028), Prevotellaceae (p ¼ .088) and Lachnospiraceae (p ¼ .068). The ruminal pH (p ¼ .023) and ammonia-N (p ¼ .031) concentration were lower in the daidzein group than in the control group, but the daidzein group tended to have greater ruminal total VFA (p ¼ .063) concentration. Supplemental daidzein increased urinary excretion of allantoin (p ¼ .005) and total purine derivatives (p ¼ .007). Current results suggest that supplemental daidzein can affect ruminal fermentation by changing ruminal microbial community, increase the production of VFA and enhance microbial protein synthesis in the rumen, showing the potential of daidzein for improving ruminal fermentation in beef cattle.

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Effect of pH on biodiesel production and the microbial structure of glucose-fed activated sludge

Cited by 31 publications

References 22 publications

Polysaccharides from Chrysanthemum morifolium Ramat ameliorate colitis rats by modulating the intestinal microbiota community

Polysaccharides from Chrysanthemum morifolium Ramat ameliorate colitis rats by modulating the intestinal microbiota community

Deciphering bacterial community changes in zucker diabetic fatty rats based on 16S rRNA gene sequences analysis

Response of rumen bacterial diversity and fermentation parameters in beef cattle to diets containing supplemental daidzein

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