Effect of pH and nitrite concentration on nitrite oxidation rate

Jiménez, E.; Giménez, Juan Bautista; Ruano, M.V.; Ferrer, J.; Serralta, J.

doi:10.1016/j.biortech.2011.07.092

Cited by 61 publications

(38 citation statements)

References 19 publications

(7 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…were 7.9-8.2 and 7.2-7.6, respectively [51]. The 100% inhibition of NOB activity was observed at pH less than 6.5 [52]. The specific growth rate of ammonium oxidizers and nitrite oxidizers decreased as the pH increases up 9-9.5 [53].…”

Section: Nitrification Processmentioning

confidence: 97%

Review on the Fate and Mechanism of Nitrogen Pollutant Removal from Wastewater Using a Biological Filter

Wang¹,

Zhi²,

Deng³

et al. 2017

Pol. J. Environ. Stud.

View full text Add to dashboard Cite

Section: Nitrification Processmentioning

confidence: 97%

Review on the Fate and Mechanism of Nitrogen Pollutant Removal from Wastewater Using a Biological Filter

Wang¹,

Zhi²,

Deng³

et al. 2017

Pol. J. Environ. Stud.

View full text Add to dashboard Cite

“…NOB activity is strongly influenced by pH, since in values below 6.5 these organisms activity virtually ceases. According to Jiménez et al (2011), optimal values for these bacteria activity lie in a range of 7.5 to 9.95. Villaverde (2004) reports, that at pH below 6.5 the nitrification process practically stops by lack of free ammonia in the environment and nitrous acid high concentrations.…”

Section: Resultsmentioning

confidence: 99%

Nitrifying, Denitrifying and Heterotrophic Biomass Present in Moving Bed-Reactor

Oliveira¹,

Correa²,

Prates³

et al. 2017

American Journal of Environmental Sciences

View full text Add to dashboard Cite

This study was carried out to evaluate the main bacterial communities related to the removal of nitrogen in a moving bed-reactor treating landfill leachate, relating the physico-chemical parameters with the existence of these organisms in the mixed liquor (non-attached microorganisms) and Support Material (SM). The system was operated in two phases: Phase I (without effluent recirculation) and II (with recirculation, at a flow rate of 3 times the inlet flow). To monitor the system, physico-chemical analyzes were determined in the influent and effluent: pH, alkalinity, temperature, nitrogen species, Chemical Oxygen Demand (COD) and Biochemical Oxygen Demand (BOD). To determine the concentration of nitrifying bacteria (Ammonia Oxidizing Bacteria-AOB and Nitrite Oxidizing Bacteria-NOB) and denitrifiers, the most probable number was estimated per 100 mL (MPN.100 mL −1 ). The concentration of heterotrophic bacteria was estimated by determination of colony forming unit per mL (CFU.mL −1 ). The reactor showed a high percentage of NH 4 + -N removal in both phases of operation, reaching 80% removal efficiency in Phase I and 83% in II. At pH close to 5.4 NOB activity was practically ceased, with nitrite accumulation in the system. Although the oxygen concentration in the mixed liquor was above 2.0 mg.L −1 the concentration of denitrifying bacteria was not affected. The concentration of heterotrophic bacteria was above 10 9 CFU.mL −1 , but COD removal in the system was low due to low BOD/COD ratio in the mixed liquor. Analyzing the physicochemical results and correlating them with the microbiological, it is verified that the MPN.100 mL −1 of the nitrifying organisms were strongly affected by the effluent conditions, being necessary for an effective nitrification process the control of these parameters, mainly pH.

show abstract

“…According to Strauss et al (44), pH is one of the most important variables for regulating nitrification, and a pH of 6.5 can completely inhibit NOB activity (45). To elucidate the metabolic activity of biofilm inhabiting NOB in dependency on pH, we performed laboratory scale short-term nitrite consumption experiments at different pH values with biofilm material from BF2 (September 2011).…”

Section: What Controls Nitrification In Ras?mentioning

confidence: 99%

Relative Abundance of Nitrotoga spp. in a Biofilter of a Cold-Freshwater Aquaculture Plant Appears To Be Stimulated by Slightly Acidic pH

Hüpeden

Wegen

Off

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

The functioning of recirculation aquaculture systems (RAS) is essential to maintain water quality for fish health, and one crucial process here is nitrification. The investigated RAS was connected to a rainbow trout production system and operated at an average temperature of 13°C and pH 6.8. Community analyses of the nitrifying biofilm revealed a coexistence of Nitrospira and Nitrotoga, and it is hypothesized that a slightly acidic pH in combination with lower temperatures favors the growth of the latter. Modification of the standard cultivation approach toward lower pH values of 5.7 to 6.0 resulted in the successful enrichment (99% purity) of Nitrotoga sp. strain HW29, which had a 16S rRNA sequence similarity of 99.0% to Nitrotoga arctica. Reference cultures of Nitrospira defluvii and the novel Nitrotoga sp. HW29 were used to confirm differentiation of these nitrite oxidizers in distinct ecological niches. Nitrotoga sp. HW29 revealed pH and temperature optima of 6.8 and 22°C, respectively, whereas Nitrospira defluvii displayed the highest nitrite oxidation rate at pH 7.3 and 32°C. We report here the occurrence of Nitrotoga as one of the main nitrite-oxidizing bacteria in freshwater aquaculture systems and indicate that a slightly acidic pH, in addition to temperatures below 20°C, can be applied as a selective isolation criterion for this microorganism. Nitrogen is one of the most important elements of life and nitrification is a key process in the global N cycle, since the biological conversion of ammonia to nitrate is required wherever organic matter accumulates. This oxidation of ammonia to nitrate via nitrite is catalyzed by chemolithoautotrophic ammonia-oxidizing bacteria (AOB) and archaea (AOA) and nitrite-oxidizing bacteria (NOB). The biotechnological application of nitrification in recirculating aquaculture systems (RAS) has gained increasing attention for the production of aquatic organisms, since it allows an efficient and ecological way of fish breeding. To remove toxic ammonia released by fish gills or mineralization of feces and feed offcut, biological filtration is required. Nitrification is promoted on the surfaces of carrier elements in well-aerated moving-bed reactors. The carrier elements (plastic beads) are colonized by nitrifying bacteria, which form a dense biofilm together with heterotrophic bacteria.In biofilters of most aquaculture systems (marine and freshwater), Nitrosomonas and Nitrospira were identified as the main ammonia and nitrite oxidizers, respectively (1-4), but members of the genera Nitrobacter and Nitrotoga are also potential candidates for nitrite removal (5, 6). Cold-adapted Nitrotoga spp. were initially found in permafrost-affected soils in Siberia (7), and the preference for low temperatures was confirmed by the detection of Nitrotoga-like 16S rRNA gene sequences in other cold soils such as recently deglaciated soils (8) or periglacial soils (9). Nitrotoga was also abundant in oligotrophic aquatic environments such as the Movile cave (10), a neutral groundwater seep (11) ...

show abstract

Effect of pH and nitrite concentration on nitrite oxidation rate

Cited by 61 publications

References 19 publications

Review on the Fate and Mechanism of Nitrogen Pollutant Removal from Wastewater Using a Biological Filter

Review on the Fate and Mechanism of Nitrogen Pollutant Removal from Wastewater Using a Biological Filter

Nitrifying, Denitrifying and Heterotrophic Biomass Present in Moving Bed-Reactor

Relative Abundance of Nitrotoga spp. in a Biofilter of a Cold-Freshwater Aquaculture Plant Appears To Be Stimulated by Slightly Acidic pH

Contact Info

Product

Resources

About