Introduction and hypothesis: Theoretically, tight or strong pelvic floor muscles may impair the progress of labor and lead to instrumental deliveries. We aimed to investigate whether vaginal resting pressure, pelvic floor muscle strength or endurance at mid-pregnancy affect delivery outcome. Methods: this was a prospective cohort study of women giving birth at a university hospital. Vaginal resting pressure, pelvic floor muscle strength and endurance in 300 nulliparous pregnant women were assessed at mean gestational week 20.8 (±1.4) using a high precision pressure transducer connected to a vaginal balloon. Delivery outcome measures (acute cesarean section, prolonged second stage of labor (> 2 hours), instrumental vaginal delivery (vacuum and forceps), episiotomy and 3 rd and 4 th degree perineal tear) were retrieved from the hospital's electronic birth records. Results: Twenty-three women were lost to follow-up, mostly because they gave birth at another hospital. Women with prolonged second stage had significantly higher resting pressure than women with second stage less than 2 hours; mean difference 4.4 cm H 2 O (95% CI: 1.2-7.6), p<0.01, aOR: 1.049 (95% CI: 1.011-1.089, p=0.012). Vaginal resting pressure did not affect other delivery outcomes. Pelvic floor muscle strength and endurance similarly were not associated with any delivery outcomes. Conclusions: While mid-pregnancy vaginal resting pressure is associated with prolonged second stage of labor, neither vaginal resting pressure nor pelvic floor muscle strength or endurance are associated with operative delivery or perineal tears. Strong pelvic floor muscles are not disadvantageous for vaginal delivery.