Effect of passive smoking on blood lymphocyte apoptosis in children

El-Hodhod, M. A.; Hamdy, Ahmed; Ahmed, Manal Basyouni; Youssef, Soha R.; Aly, Shaimaa M. H.

doi:10.1111/j.1365-2362.2010.02420.x

Cited by 13 publications

(6 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with previous studies [ 9 , 27 , 28 ], the current study demonstrated that CS exposure could impair the plasma lipid profiles. Interestingly, we also found that H 2 treatment significantly improved the levels of plasma HDL-C, LDL-C and TC compared with the CS group.…”

Section: Discussionsupporting

confidence: 92%

Cigarette smoke exposure impairs reverse cholesterol transport which can be minimized by treatment of hydrogen-saturated saline

et al. 2015

View full text Add to dashboard Cite

BackgroundCigarette smoke (CS) exposure impaired plasma lipid profiles by modification of apolipoproteins. Hydrogen (H2) has been proved effective on reducing oxidative stress or improving HDL functionalities in animal models or metabolic syndrome volunteers. This study was undertaken to explore the effects of CS exposure on reverse cholesterol transport (RCT) and the antioxidative effects of H2 treatment against CS exposure in mice transgenic for human cholesteryl ester transfer protein (CETP).Methods[3H]-cholesterol-laden macrophages were injected intraperitoneally into mice, and the samples of blood, bile, liver, and feces were collected for radioactivity determination to evaluate RCT. [3H]-cholesterol-laden macrophages were incubated with HDL isolated from different groups of mice, and the samples of cell medium supernatants were collected for evaluating the HDL functionality to elicit cholesterol efflux.ResultsCS exposure significantly decreased plasma HDL cholesterol level (HDL-C) by 22 % and increased LDL cholesterol level (LDL-C) by 21 % compared with the control group (p < 0.05, p < 0.01), while H2 treatment significantly improved the CS-impaired levels of TC, LDL-C and HDL-C by 10, 27 and 31 %, respectively, compared with the CS group (p < 0.05, p < 0.01 and p < 0.05). Besides, CS exposure significantly decreased [3H] tracer concentrations in liver, bile and feces by 17, 35 and 48 %, respectively, compared with the control group (p < 0.05 for liver and feces), while H2 treatment significantly improved them by 21, 72 % and 89 %, respectively, compared with the CS group (all p < 0.05). Furthermore, CS exposure significantly decreased the HDL functionality to elicit cholesterol efflux by 26 % (p < 0.05), while H2 treatment also improved it by 32 % (p < 0.05). We did not find any significant alterations in protein expressions of RCT involved genes.ConclusionsThese findings provided direct evidence supporting the notion that CS exposure in vivo impairs plasma lipid profiles, HDL functionalities and macrophage-to-feces RCT pathway in CETP transgenic mice, all of which can be minimized by treatment of H2-saturated saline.

show abstract

Section: Discussionsupporting

confidence: 92%

Cigarette smoke exposure impairs reverse cholesterol transport which can be minimized by treatment of hydrogen-saturated saline

et al. 2015

View full text Add to dashboard Cite

show abstract

“…7 Thus, Tibetan children have a greater risk of exposure to SHS. Exposure to tobacco smoke may lead to alterations in the serum lipid profile, especially to a decrease in high-density lipoprotein (HDL) cholesterol, in children 8,9 and adolescents. 10 However, no study has focused on the vascular endothelial function and atherosclerosis of Tibetan adolescents exposed to passive smoking.…”

Section: Introductionmentioning

confidence: 99%

Deterioration of endothelial function and carotid intima-media thickness in Tibetan male adolescents exposed to second-hand smoke

Yang

Chen

et al. 2012

J Renin Angiotensin Aldosterone Syst

View full text Add to dashboard Cite

Introduction: It has been commonly accepted that second-hand smoke (SHS) is associated with atherosclerosis and endothelial dysfunction. There is growing evidence that the changes might begin in childhood. Unfortunately, no study has focused on the early atherosclerosis of Tibetan adolescents exposed to SHS. Aims: We aimed to investigate the endothelial function and carotid atherosclerosis in healthy school-aged Tibetan male adolescents. Materials and methods: All passive smoking participants (SHS) were students were 16 years old and male, and were recruited through middle schools in Lhasa city. In total 624 subjects were accepted after excluding subjects who actively smoked. The adolescents were divided into three groups according to serum cotinine level: high cotinine group (High Group) with 205 boys, intermediate cotinine group (Intermediate Group) with 210 boys, and low cotinine group (Low Group) with 209 boys. Venous blood was sampled for the measurement of cotinine concentration, lipid profile and endothelin-1 (ET-1) quantitation. High-resolution B-mode ultrasonography was performed to evaluate carotid intimamedia thickness (cIMT) and intima smoothness. The invasive vascular endothelial function was evaluated through the measurement of flow-mediated dilation (FMD) with B-mode ultrasound and ankle-brachial index (ABI) by using a blood pressure cuff and a Doppler instrument. Results: No statistical significance was found between groups in total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triglyceride, ApoA-I, systolic blood pressure, diastolic blood pressure, and heart rate (p>0.05). In the lipid profile, only apolipoprotein B (ApoB) values were different between groups: ApoB in the High Group was higher than in the Low Group (p=0.0164). Plasma ET-1 concentrations in the High Group were also much higher than in the Intermediate and Low Groups (p=0.0112, p<0.001). The cIMT and intima smoothness had deteriorated in the High Group compared with the Low Group (p<0.001 and p<0.05 respectively). FMD and ABI, which indicate vascular endothelial function, was decreased in the High Group compared with the Intermediate and Low Groups (FMD, p<0.001; ABI, p<0.001). Conclusions: SHS was associated with sub-clinical carotid atherosclerosis and endothelial dysfunction in Tibetan schoolaged male adolescents. Considering the widespread exposure to SHS and the clinical relevance of early atherosclerosis, this result is of public health importance in Tibet, where health education is not satisfactory. Data from our study emphasize the importance of endorsing smoke-free environments for adolescents.

show abstract

“…There was no observable effect of patient smoking habit on lymphocyte apoptosis at 0 Gy in our data, suggesting smoking has an interactive rather than a direct effect with radiation. This observation helps explain the apparent contradiction with published laboratory studies on the effect of smoking on lymphocyte apoptosis [27], [28], [29]. The observation of reduced radiation-induced apoptosis in smokers does however concord with the hypothesis that low apoptosis is associated with increased radiation toxicity, because smoking has been identified as a risk factor for toxicity by published clinical studies in a range of cancer types [30], [31], [32], [33], [34].…”

Section: Discussionmentioning

confidence: 60%

Multi-centre technical evaluation of the radiation-induced lymphocyte apoptosis assay as a predictive test for radiotherapy toxicity

Talbot

Veldwijk

Azria

et al. 2019

Clinical and Translational Radiation Oncology

View full text Add to dashboard Cite

Highlights The RILA assay is the leading candidate biomarker for radiotherapy toxicity. We describe work to standardise its use across multiple centres. Patient factors including smoking and arthritis were found to affect RILA score. RILA predicts acute breast pain but not other acute end-points. This work establishes the basis for implementing the assay clinically.

show abstract

Effect of passive smoking on blood lymphocyte apoptosis in children

Abstract: Passive smoking in children could be a risk factor for enhanced lymphocytic apoptosis. It is possible that altered lipid profile may play a role in the increased risk. The impact of this lymphocytic derangement on increased frequency of infections is noticeable.

Cited by 13 publications

References 35 publications

Cigarette smoke exposure impairs reverse cholesterol transport which can be minimized by treatment of hydrogen-saturated saline

Cigarette smoke exposure impairs reverse cholesterol transport which can be minimized by treatment of hydrogen-saturated saline

Deterioration of endothelial function and carotid intima-media thickness in Tibetan male adolescents exposed to second-hand smoke

Multi-centre technical evaluation of the radiation-induced lymphocyte apoptosis assay as a predictive test for radiotherapy toxicity

Contact Info

Product

Resources

About