Effect of passage number and matrix characteristics on differentiation of endothelial cells cultured for tissue engineering

Chennazhy, Krishna Prasad; Krishnan, Lissy K.

doi:10.1016/j.biomaterials.2005.02.024

Cited by 85 publications

(53 citation statements)

References 25 publications

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“…Endothelial cell compatibility testing of biomaterials has been similar; and involves the growth and spread of cells on the materials as well as study of the effects that the biomaterial has on endothelial cell inflammatory potential and the angiogenic potential of the cells to migrate and form microcapillary-like structures or microvessels [12,[35][36][37][38]. The bone biomaterials used in these studies are being used successfully in humans.…”

Section: Article In Pressmentioning

confidence: 98%

Tissue-like self-assembly in cocultures of endothelial cells and osteoblasts and the formation of microcapillary-like structures on three-dimensional porous biomaterials

et al. 2007

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Section: Article In Pressmentioning

confidence: 98%

Tissue-like self-assembly in cocultures of endothelial cells and osteoblasts and the formation of microcapillary-like structures on three-dimensional porous biomaterials

et al. 2007

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“…13 One milliliter of the composed matrix comprised 5 mg in-house isolated human cryoprecipitate (clottable fibrinogen and fibronectin), 0.2% gelatin (Sigma), and 100 μg hyaluronic acid (in-house purified and characterized 14 ), 20 μg of released platelet growth factor (PGF) prepared per Resmi et al, 15 25 μg laminin V (Sigma), and 250 ng recombinant epidermal growth factor (EGF; Sigma). Briefly, matrix composite was clotted on thrombin adsorbed TCPS to get a thin fibrin layer, and dishes were lyophilized under sterile atmosphere using a freeze drier (Edwards, Modulyo 4K).…”

Section: Methodsmentioning

confidence: 99%

Constitution of Fibrin-Based Niche for In Vitro Differentiation of Adipose-Derived Mesenchymal Stem Cells to Keratinocytes

Unnikrishnan

Jayakumar

Krishnan

2014

BioResearch Open Access

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Epithelialization of chronic cutaneous wound is troublesome and may require use of skin/cell substitutes. Adipose-derived mesenchymal stem cells (ADMSCs) have immense potential as autologous cell source for treating wounds; they can cross the germ layer boundary of differentiation and regenerate skin. When multipotent adult stem cells are considered for skin regeneration, lineage committed keratinocytes may be beneficial to prevent undesirable post-transplantation outcome. This study hypothesized that ADMSCs may be directed to epidermal lineage in vitro on a specifically designed biomimetic and biodegradable niche. Cells were seeded on the test niche constituted with fibrin, fibronectin, gelatin, hyaluronic acid, laminin V, platelet growth factor, and epidermal growth factor in the presence of cell-specific differentiation medium (DM). The ADMSCs grown on bare tissue culture polystyrene surface in DM is designated DM-control and those grown in basal medium (BM) is the BM-control. Lineage commitment was monitored with keratinocyte-specific markers such as cytokeratin 14, cytokeratin 5, cytokeratin 19, and integrin α6 at the transcriptional/translational level. The in vitro designed biomimetic fibrin composite matrix may have potential application as cell transplantation vehicle.

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“…The sharp delineation with regard to post-treatment adhesion programs between liposuction and excision isolation techniques in age and BMI-matched donors suggests that there is an important difference in either the population of cells obtained by each method or a phenotypic change caused by one method that is not caused by the other. It is possible that cells obtained by liposuction have experienced some degree of phenotypic change caused by mechanical agitation from the liposuction cannula or infusion of large boluses of saline solution; however, it is unlikely that such a phenotypic change would persist after three passages of expansion in culture [35,36]. A more likely explanation for these observations is that there is some population present or enriched in the liposuction aspirate that is not present (or present to a lesser degree) in the panniculectomy excision.…”

Section: Discussionmentioning

confidence: 99%

Functional Binding of Human Adipose-Derived Stromal Cells

Amos

Bailey²,

Shang³

et al. 2008

Annals of Plastic Surgery

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Human adipose-derived stromal cells (hASCs) were evaluated in vitro for their ability to bind vascular adhesion and extracellular matrix proteins in order to arrest (firmly adhere) under physiological flow conditions. hASCs were flowed through a parallel plate flow chamber containing substrates presenting immobilized Type I Collagen, fibronectin, E-selectin, L-selectin, P-selectin, vascular cell adhesion molecule-1 (VCAM-1), or intercellular adhesion molecule-1 (ICAM-1) under static and laminar flow conditions (wall shear stress = 1 dyn/cm 2 ). hASCs were able to firmly adhere to Type I Collagen, fibronectin, VCAM-1, and ICAM-1 substrates, but not to any of the selectins. Pretreatment with hypoxia increased the ability of hASCs isolated by liposuction to adhere to VCAM-1 and ICAM-1, but this effect was not seen in cells isolated by tissue excision. These results indicate that hASCs possess the ability to adhere key adhesion proteins, illustrate the importance of hASC harvest procedure, and suggest mechanisms for homing in a setting where interaction with inflamed or injured tissue is necessary.

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Effect of passage number and matrix characteristics on differentiation of endothelial cells cultured for tissue engineering

Cited by 85 publications

References 25 publications

Tissue-like self-assembly in cocultures of endothelial cells and osteoblasts and the formation of microcapillary-like structures on three-dimensional porous biomaterials

Tissue-like self-assembly in cocultures of endothelial cells and osteoblasts and the formation of microcapillary-like structures on three-dimensional porous biomaterials

Constitution of Fibrin-Based Niche for In Vitro Differentiation of Adipose-Derived Mesenchymal Stem Cells to Keratinocytes

Functional Binding of Human Adipose-Derived Stromal Cells

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