Effect of particle size on the rate of enzymatic hydrolysis of cellulose

Yeh, An‐I; Huang, Yi‐Ching; Chen, Shih-Hsin

doi:10.1016/j.carbpol.2009.07.049

Cited by 214 publications

(115 citation statements)

References 32 publications

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“…A good linear relationship between yield of reducing sugar and hydrolysis time was obtained using the data collected within 4 h. Thus, the initial hydrolysis reaction rate (V0, mg/L·h) was calculated as the slope of linear curve fitted by reducing sugar yield data of 0 (=0), 1, 2, 4 h. This method was used in previous studies (Harun and Danquah 2011;Yeh et al 2010). Figure 1 presents the cellulose contents of various biomass materials used in this study.…”

Section: Determination Of Reducing Sugar and Initial Hydrolysis Reactmentioning

confidence: 95%

Relationship between Crystallinity Index and Enzymatic Hydrolysis Performance of Celluloses Separated from Aquatic and Terrestrial Plant Materials

Li¹,

Zhou²,

Wu³

et al. 2014

BioResources

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a Hydrolysis experiments of five cellulose samples (separated from two aquatic plants and three terrestrial plants, respectively) were conducted at various cellulase loadings (7 to 200 FPU/g cellulose). No obvious correlation was found between CrI and hydrolysis performance at low enzyme loadings (e.g. 7 and 28 FPU/g cellulose), as the hydrolysis was controlled by enzyme availability and the differences in cellulose structure were unimportant. At a sufficiently high enzyme loading (e.g. 200 FPU/g cellulose), the yield of reducing sugar was linearly proportional to the CrI value. Therefore, to establish such a correlation between cellulose structure and hydrolysis performance, hydrolysis experiments must be conducted under the conditions where enzyme availability is not a limiting factor. It was found that celluloses from sugarcane bagasse and water hyacinth have low CrI, achieve high sugar yields, exhibit fast reactions during enzymatic hydrolysis at low enzyme loadings, and can potentially be good feedstocks for bio-ethanol production.

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Section: Determination Of Reducing Sugar and Initial Hydrolysis Reactmentioning

confidence: 95%

Relationship between Crystallinity Index and Enzymatic Hydrolysis Performance of Celluloses Separated from Aquatic and Terrestrial Plant Materials

Li¹,

Zhou²,

Wu³

et al. 2014

BioResources

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“…This behavior is easily visualized including the aforementioned pores radius in the expression P = 2γ/r, where P is the excess of pressure that leads to the capillary rise, γ the surface tension of the liquid and finally, r the pore radius. In the three pores sizes, the water vapor passage is possible [7] [8]…”

Section: Introductionmentioning

confidence: 99%

Low Density Wood Impregnation with Water-Repellent Organosilicic Compounds

Canosa¹,

Alfieri²,

Giúdice³

2018

MSCE

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Many protective treatments for low density wood are applied by impregnation to give water-repellency and to control pathologies that usually have this substrate. The properties of Araucaria angustifolia, chemically modified by impregnation with methyltriethoxysilane, n-octyltriethoxysilane and mixtures of both in several ratios, were investigated to achieve mainly high dimensional stability, low capillary water absorption as well as satisfactory water vapor permeability. The aforementioned impregnants produce the wood chemical modification, involving the reaction of hydroxyl groups of the wood with the hydrolysis products of alcoxysilanes. It is concluded that the organosilicon polymers allow improving important characteristics of wood: 1) the non-occlusive coating keeps the water vapor permeability unaltered; 2) the alkoxysilane type defines the hydrophobicity and the continuity of coating formed on the pore wall and finally; 3) the polymeric structure formed after finishing sol-gel process incises both on the capillary water absorption and the dimensional stability. In addition, the studied treatments have the advantage of allowing that the water vapor, which permeates through the organosilicon coating placed on cell wall, can exit by hydrophobic repulsion and thus, prevent faults appearance generated by the condensed water inside of wood.

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“…After this preincubation step, hydrolysis was initiated by adding 0.1 ml of 10 mg/mL cellulase (activity: 700 EGU/g). 40 μL of the reaction medium was withdrawn at different times in order to determine the progress of the reaction which was stopped by incubating the withdrawn sample at 90 o C for 20 min to deactivate the enzyme [9]. Then, the sample was diluted in ultra-pure water and filtered (0.2 μm) prior to analysis by high performance liquid chromatography (HPLC).…”

Section: Enzymatic Hydrolysis Of Opefb Cellulosementioning

confidence: 99%

“…This is probably due to the decrease in spiral angle around the fibre axis and increase in molecular orientation after pretreatment. A fair amount of randomness is introduced in the orientation of the crystallites due to the removal of non-cellulosic matter [13]. …”

Section: Morphology Of Opefb Fibersmentioning

confidence: 99%

Production of succinic acid from oil palm empty fruit bunch cellulose using Actinobacillus succinogenes

Pasma

Daik²,

Maskat³

2013

AIP Conference Proceedings

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Abstract. Succinic acid is a common metabolite in plants, animals and microorganisms. It has been used widely in agricultural, food and pharmaceutical industries. Enzymatic hydrolysate glucose from oil palm empty fruit bunch (OPEFB) cellulose was used as a substrate for succinic acid production using Actinobacillus succinogenes. Using cellulose extraction from OPEFB can enhance the production of glucose as a main substrate for succinic acid production. The highest concentration of glucose produced from enzymatic hydrolysis is 167 mg/mL and the sugar recovery is 0.73 g/g of OPEFB. By optimizing the culture medium for succinic acid fermentation with enzymatic hydrolysate of OPEFB cellulose, the nitrogen sources could be reduced to just only 2.5 g yeast extract and 2.5 g corn step liquor. Batch fermentation was carried out using enzymatic hydrolysate of OPEFB cellulose with yeast extract, corn steep liquor and the salts mixture, 23.5 g/L succinic acid was obtained with consumption of 72 g/L glucose in enzymatic hydrolysate of OPEFB cellulose at 38 hours and 37 o C. This study suggests that enzymatic hydrolysate of OPEFB cellulose maybe an alternative substrate for the efficient production of succinic acid by Actinobacillus succinogenes.

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Effect of particle size on the rate of enzymatic hydrolysis of cellulose

Cited by 214 publications

References 32 publications

Relationship between Crystallinity Index and Enzymatic Hydrolysis Performance of Celluloses Separated from Aquatic and Terrestrial Plant Materials

Relationship between Crystallinity Index and Enzymatic Hydrolysis Performance of Celluloses Separated from Aquatic and Terrestrial Plant Materials

Low Density Wood Impregnation with Water-Repellent Organosilicic Compounds

Production of succinic acid from oil palm empty fruit bunch cellulose using Actinobacillus succinogenes

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