Effect of Parathyroid Hormone on Osmotic Fragility of Human Erythrocytes

Bogin, E.; Massry, Shaul G.; Levi, J; Djaldeti, Meir; Bristol, Gregory; Smith, Jacqueline

doi:10.1172/jci110505

Cited by 184 publications

(107 citation statements)

References 36 publications

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“…Even if such a possibility does exist, one must still conclude that the excess blood levels of PTH are of paramount importance in the genesis ofthe reduced RBC survival in uremia. Our previous studies (10) demonstrating that both the intact PTH (1-84 PTH) and its amino-terminal fragment (1-34 PTH) increased osmotic fragility of human RBC and caused their hemolysis are consistent with the notion that excess blood levels of PTH in chronic renal failure have a direct effect on RBC survival.…”

Section: Discussionsupporting

confidence: 80%

“…The mechanisms of action of PTH on human RBC have been previously investigated in our laboratory (10,16). These studies showed that the effect of PTH on osmotic fragility is due to alteration in the shape of the RBC; this effect is dependent on enhanced calcium entry in RBC and on stimulation of Caactivated ATPase of RBC membrane (10).…”

Section: Discussionmentioning

confidence: 99%

“…We have previously demonstrated that the RBC is a target organ for parathyroid hormone (PTH) and the latter increases osmotic fragility of RBC and induces hemolysis (10). Patients with advanced renal failure have secondary hyperparathyroidism and elevated blood levels of PTH (1 1-13).…”

Section: Introductionmentioning

confidence: 99%

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Erythrocyte survival in chronic renal failure. Role of secondary hyperparathyroidism.

Akmal¹,

Telfer²,

Ansari³

et al. 1985

J. Clin. Invest.

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The human erythrocyte (RBC) is a target organ for parathyroid hormone (PTH) and the hormone increases RBC osmotic fragility and induces their hemolysis. The present study was undertaken to examine whether elevated blood levels of PTH affect RBC survival, and therefore whether PTH, being an extracorpuscular factor, is responsible for the shortened RBC survival in chronic renal failure. 51Cr-labeled RBC survival was elevated in six normal dogs, in six animals with chronic renal failure and secondary hyperparathyroidism (NPX), and in six thyroparathyroidectomized dogs (NPX-TPTX) with comparable degree and duration of chronic renal failure. In the normal dogs, 51Cr-labeled RBC survival ranged between 22 and 35 (25.6±1.9) d. In the NPX dogs, 51Cr-labeled RBC survival was shortened and the values ranged between 16 and 20 (18.4±0.6) d, a value significantly (P < 0.01) lower than normal dogs. In NPX-TPTX dogs, 5tCr-labeled RBC survival ranged between 20 and 33 (25.2±1.8) d, a value not different from that in normal dogs but significantly higher (P < 0.01) than that in NPX animals.Our data demonstrate that excess blood levels of PTH and not other consequences of the uremic state are responsible for the shortened RBC survival in chronic renal failure.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

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Erythrocyte survival in chronic renal failure. Role of secondary hyperparathyroidism.

Akmal¹,

Telfer²,

Ansari³

et al. 1985

J. Clin. Invest.

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“…Hyperparathyroidism has been associated with hypertension (29), and it increases calcium uptake in human cells (30). Parathyroidectomy after primary hyperparathyroidism improves blood pressure and arterial smooth muscle (31,32).…”

Section: Discussionmentioning

confidence: 99%

Effects of a Short-Term Vitamin D3 and Calcium Supplementation on Blood Pressure and Parathyroid Hormone Levels in Elderly Women

Pfeifer¹

2001

Journal of Clinical Endocrinology & Metabolism

375

350

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Calcium supplementation is effective in reducing blood pressure in various states of hypertension, including pregnancy-induced hypertension and preeclampsia. In addition, calcitropic hormones are associated with blood pressure. The hypothesis is that short-term therapy with calcium and vitamin D 3 may improve blood pressure as well as secondary hyperparathyroidism more effectively than calcium monotherapy.The effects of 8 weeks of supplementation with vitamin D 3 (cholecalciferol) and calcium on blood pressure and biochemical measures of bone metabolism were studied. The sample consisted of 148 women (mean Ϯ SD age, 74 Ϯ 1 yr) with a 25-hydroxycholecalciferol (25OHD 3 ) level below 50 nmol/L. They received either 1200 mg calcium plus 800 IU vitamin D 3 or 1200 mg calcium/day. We measured intact PTH, 25OHD 3 , 1,25-dihydroxyvitamin D 3 , blood pressure, and heart rate before and after treatment.Compared with calcium, supplementation with vitamin D 3 and calcium resulted in an increase in serum 25OHD 3 of 72% (P Ͻ 0.01), a decrease in serum PTH of 17% (P ϭ 0.04), a decrease in systolic blood pressure (SBP) of 9.3% (P ϭ 0.02), and a decrease in heart rate of 5.4% (P ϭ 0.02). Sixty subjects (81%) in the vitamin D 3 and calcium group compared with 35 (47%) subjects in the calcium group showed a decrease in SBP of 5 mm Hg or more (P ϭ 0.04). No statistically significant difference was observed in the diastolic blood pressures of the calcium-treated and calcium-plus vitamin D 3 -treated groups (P ϭ 0.10). Pearson coefficients of correlation between the change in PTH and the change in SBP were 0.49 (P Ͻ 0.01) for the vitamin D 3 plus calcium group and 0.23 (P Ͻ 0.01) for the calcium group. 1-3). In addition, other reports suggest that calcium intake may have effects in a variety of unrelated diseases such as arterial hypertension (4 -7), cancer of the colon (8, 9), and prevention of nephrolithiasis (10). Furthermore, an association of 1,25-dihydroxyvitamin D 3 [1,25-(OH) 2 D 3 ] and blood pressure has been shown in normotensive men (11), myocardial infarction was inversely associated with plasma 25-hydroxyvitamin D 3 (25OHD 3 ) levels in a community-based study (12), and higher blood pressure in elderly women correlated to increased bone loss in a recently published prospective study (13). On the other hand, dietary calcium intake fails to meet recommended levels in virtually all categories of Americans (14, 15), and hypovitaminosis D affects both Americans (16) and Europeans (17).We have previously shown that short-term supplementation with vitamin D 3 and calcium improves body sway and secondary hyperparathyroidism and therefore prevents falls and subsequent nonvertebral fractures in elderly women (18,19). We have now extended our studies to the effects of short-term supplementation with vitamin D 3 and calcium on blood pressure, as vascular smooth muscle is a target organ for vitamin D (20). The role of calcitropic hormones in the regulation of blood pressure is unclear (21-23). To our knowledge this is the ...

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“…This is in part accounted for by the reduced erythropoietin biosynthesis of the diseased kidney. The reduced osmotic resistance of erythrocytes in cases of elevated plasma concentrations of parathyrin might also contribute to anaemia (6). Erythropoietin produced by genetic engineering techniques can now be sed to treat anaemia in haemodialysis patients.…”

mentioning

confidence: 99%

Changes in the Concentrations of Hydroxyproline, Glycine and Serine in the Plasma of Haemodialysis Patients Undergoing, Erythropoietin Therapy

Riedel¹,

Nündel²,

Algermissen³

et al. 1989

Clinical Chemistry and Laboratory Medicine

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Summary:The concentrations of proline, hydroxyproline, glycine and serine were determined in the plasma of 39 haemodialysis patients and 18 healthy subjects, using liquid chromatography with fluorescence detection. Plasma cpncentrations of the N-terminal immunoreactive parathyrin were also measured. In haemodialysis patients, the plasma concentrations of glycine (p < 0.01), hydroxyproline (p < 0.05) and proline (p < 0.10) were significantly increased, whereas the serine concentrations (p < 0.01) were decreased, compared with those of the healthy controls. Haemodialysis patients showed greatly elevated plasma N-terminal immunoreactive parathyrin valües (> 30 pmol/1), which showed a signiflcant correlation with the hydroxyproline values (r = 0.79). Fourteen haemodialysis patients received erythropoietin therapy. In these patients, changes in the concentrations öf plasma amino acids were observed up to one year after the beginning of therapy. In the course of the erythroppietin therapy, the plasma cöücentrations of glycine (p < 0.05) and hydroxyproline (p < 0.10) of the haemodialysis patients decreased, whereas the concentration of serine increased (p < 0.05) to approximately normal values. The resülts indicate that erythropoietin therapy leads to a normalization of amino acid metabolism.

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Effect of Parathyroid Hormone on Osmotic Fragility of Human Erythrocytes

Cited by 184 publications

References 36 publications

Erythrocyte survival in chronic renal failure. Role of secondary hyperparathyroidism.

Erythrocyte survival in chronic renal failure. Role of secondary hyperparathyroidism.

Effects of a Short-Term Vitamin D3 and Calcium Supplementation on Blood Pressure and Parathyroid Hormone Levels in Elderly Women

Changes in the Concentrations of Hydroxyproline, Glycine and Serine in the Plasma of Haemodialysis Patients Undergoing, Erythropoietin Therapy

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