Effect of parameters on the quality of core‐shell fibrous scaffold for retinal differentiation of conjunctiva mesenchymal stem cells

Abstract

This article describes the coaxial electrospinning to generate core-shell fibers from polycaprolactone (PCL) and polyethylene glycol (PEG) for differentiation of conjunctiva mesenchymal stem cells (CJMSCs) into photoreceptor-like cells by delivery of taurine. Also, the effects of many parameters such as polymer concentration, nozzle collector distance, applied voltage, and outer solution flow rate on creation of the core-shell structure were examined. The morphology and structure of fibers were characterized using scanning, transmission electron microscopy, and fourier transform infrared spectroscopy and then retinal differentiation was examined by quantitative real time PCR (qPCR). Significant variations between 25% and 35% PEG concentration groups for fiber diameter were documented. As faster flowing rates from the outer nozzle (PCL fluid) were applied, the creation possibility of fibrous scaffold was increased. The lowest diameter and the best quality alignment of core-shell fibrous scaffold were achieved in 22 Kv and 24 Kv. As rising distancing were applied, the fibrous diameter increased and spraying was observed. qPCR analysis demonstrated the differentiation of CJMSCs to photoreceptor like cells on PEG/PCL scaffolds. According to the result, we have proved successful in the creation of core/shell fibrous scaffold of PEG/PCL by coaxial electrospinning for retinal tissue engineering. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 189-197, 2017.

Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

“…In this study, a conductive scaffold consist of SF/rGo was fabricated by electrospinning method to evaluate electrical stimulus on the proliferation and differentiation of CJMSCs toward neural‐like cells. Electrospinning as an electrostatic technique includes the use of a high‐voltage electrostatic field to charge the polymer solution which results in the creation of solidified ultra‐thin fibers as a scaffold for biomedical and tissue engineering application . Nanofibrous scaffold gives an excellent opportunity to the tissue engineering field because of its similarity to native ECM .…”

“…Electrospinning as an electrostatic technique includes the use of a high-voltage electrostatic field to charge the polymer solution which results in the creation of solidified ultra-thin fibers as a scaffold for biomedical and tissue engineering application. 21,22,43 Nanofibrous scaffold gives an excellent opportunity to the tissue engineering field because of its similarity to native ECM. 44 To date, synthetic and natural biomaterials have been used for scaffold fabrication.…”

“…The isolated stromal tissue was incubated in DMEM/F‐12 supplemented with 10% FBS, 10 ng/mL human LIF (Chemicon, Temecula, CA, USA), 4 ng/mL basic‐FGF (Peprotech, Rocky Hill, NJ, USA), and 5 mg/mL insulin (Sigma). To identify the nature of cells, they were induced with osteogenic (DMEM including 10 nM dexamethasone [Sigma], 10 mM b‐glycerophosphate [Sigma], 50 mg/mL ascorbic acid 2‐phosphate [Sigma]), adipogenic (DMEM supplemented with 50 mg/mL indomethacine [Sigma], and 100 nM dexamethasone [Sigma]), chondrogenic (DMEM supplemented with 10 ng/mL transforming growth factor‐ß3 [TGF‐ß3; Sigma], 50 mg/mL ascorbate‐2‐phosphate [Sigma], 10‐7 M dexamethasone [Sigma], bone morphogenetic protein‐6 [BMP‐6], and 50 ug/mL insulin‐transferrin‐selenium [ITS; GIBCO]) medium for 21 days …”

Fibrin gel as a scaffold for photoreceptor cells differentiation from conjunctiva mesenchymal stem cells in retina tissue engineering

“…In line with previous studies, our MTT assay results illustrated that viability of CJMSCs cultured on fibrin gel significantly enhanced relative to cells cultured on TCP surface. This might be due to the polymerization processes of the fibrin gel that can facilitate oxygen and nutrients diffusion for the transplanted cells [12,34,39,40]. In this study, we found that culture of CJMSCs in fibrin gel leads to promotion of CJMSCs differentiation into photoreceptor-like cells.…”

Fabrication of conductive fibrous scaffold for photoreceptor differentiation of mesenchymal stem cell

“…MTT absorbance was measured at 570 nm using a microplate reader (ELX800; BioTeK, Winooski, VT). C=C, and C-O, respectively (Elzein, Nasser-Eddine, Delaite, Bistac, & Dumas, 2004;Nadri, Nasehi, & Barati, 2017). For PPY spectra, the bandʼs absorption was located at 3,414, 1,546, 1,461, and 1,292 cm −1 , which are related to N-H, C=C, C-C, and C-C/C-N stretching vibrations, respectively (Su, Li, Yuan, & Yi, 2012).…”