Effect of palmitoleic acid on the differentiation of bovine skeletal
                    muscle satellite cells

Zhang, Junfang; Li, Qiang; Nogoy, Kim Margarette Corpuz; Sun, Jianfu; Sun, Bin; Wang, Ying; Tang, Lin; Yu, Jia; Jin, Xiaowei; Li, Xiangzi; Choi, Seong‐Ho

doi:10.5187/jast.2021.e78

Cited by 8 publications

(3 citation statements)

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“…Muscle satellite cells exhibit multipotent mesenchymal stem cell activity and are capable of forming osteocytes, adipocytes, and myocytes [ 22 ]. Our previous study showed that the lipid droplet area was significantly increased and TAG accumulation was promoted in BSCs differentiated by palmitoleic acid induction [ 23 ]. Belal et al [ 24 ] showed that long-chain fatty acids (LCFAs) induced cytosolic TAG accumulation in satellite cells and significantly enhanced DGAT2 mRNA expression; however, of the two DGAT isoforms expressed in BSCs, only DGAT2 significantly responded to LCFA treatment, indicating that DGAT2 plays a critical role in TAG accumulation in cattle.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of DGAT2 Stimulates Lipid Droplet Formation and Triacylglycerol Accumulation in Bovine Satellite Cells

Zhang

et al. 2022

Animals

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Intramuscular fat (IMF) is closely related to the tenderness, juiciness, and flavor of beef, and is an important indicator for beef quality assessment internationally. The main components of skeletal intramuscular fat (IMF) are phospholipids and triacylglycerols (TAG), and the final step of TAG biosynthesis is catalyzed by diacylglycerol acyltransferase 2 (DGAT2). To explore the effect of DGAT2 on the differentiation of bovine muscle satellite cells (BSCs) and its role in the signaling pathway related to lipid metabolism, the adenovirus overexpression and interference vector of the DGAT2 gene was constructed in this study, and the overexpression adenovirus Ad-DGAT2 and interfering adenovirus sh-DGAT2 were used to infect BSCs. Overexpression of DGAT2 resulted in a significant increase in the contents of TAG and ADP, and the mRNA and protein expression levels of PPARγ, C/EBPα, and SREBF1 (p < 0.05). Interfering with the expression of DGAT2 reduced the intracellular TAG content and lipid droplet accumulation. Furthermore, the mRNA and protein expression levels of PPARγ, C/EBPα, and SREBF1 (p < 0.05) were significantly downregulated. Transcriptome sequencing showed that a total of 598 differentially expressed genes (DEGs) were screened in BSCs infected with Ad-DGAT2, and these DEGs included 292 upregulated genes and 306 downregulated genes. A total of 49 DEGs were screened in BSCs infected with sh-DGAT2, and these DEGs included 25 upregulated and 24 downregulated genes. KEGG enrichment analysis showed that the DEGs, after overexpression of DGAT2, were mainly enriched in the PPAR signaling pathway, and the fat digestion and absorption, glycerophospholipid metabolism, fatty acid biosynthesis, and AMPK signaling pathways. The DEGs obtained after interfering with DGAT2 were mainly enriched in the metabolic pathways, such as the PPAR signaling pathway and PI3K/AKT signaling pathway. In summary, our study demonstrated that the lipid droplet formation, TAG accumulation, and adipogenic gene expression in BSCs overexpressing DGAT2 were higher than those in the control cells. These results highlight the important role of DGAT2 in regulating BSCs during adipogenic transdifferentiation and underscore the complexity of intramuscular adipogenesis.

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Section: Discussionmentioning

confidence: 99%

Overexpression of DGAT2 Stimulates Lipid Droplet Formation and Triacylglycerol Accumulation in Bovine Satellite Cells

Zhang

et al. 2022

Animals

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“…Intramuscular fat deposition is determined by both genetic and environmental factors. Intramuscular adipocytes are formed by lipid filling of fibroblasts within the muscle perimeter and consist of adipocytes distributed between muscle fibers [ 23 ]. Intramuscular adipocytes are composed mainly of phospholipids and triglycerides, the content of which is determined primarily by number and size, and provide a “marbling” site for subsequent fat deposition [ 24 ].…”

Section: Recent Research On Rumen Microbiome Contributing To Meat Qua...mentioning

confidence: 99%

— Invited Review — Translational gut microbiome research for strategies to improve beef cattle production sustainability and meat quality

Mizoguchi,

Guan

2024

Anim Biosci

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Advanced and innovative breeding and management of meat-producing animals are needed to address the global food security and sustainability challenges. Beef production is an important industry for securing animal protein resources in the world and meat quality significantly contributes to the economic values and human needs. Improvement of cattle feed efficiency has become an urgent task as it can lower the environmental burden of methane gas emissions and the reduce the consumption of human edible cereal grains. Cattle depend on their symbiotic microbiome and its activity in the rumen and gut to maintain growth and health. Recent developments in high-throughput omics analysis (metagenome, metatranscriptome, metabolome, metaproteome and so on) have made it possible to comprehensively analyze microbiome, hosts and their interactions and to define their roles in affecting cattle biology. In this review, we focus on the relationships among gut microbiome and beef meat quality, feed efficiency, methane emission as well as host genetics in beef cattle, aiming to determine the current knowledge gaps for the development of the strategies to improve the sustainability of beef production.

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“…However, in vitro studies showed the different effects of unsaturated fatty acids such as stearidonic and palmitoleic acids. While stearidonic acid inhibits adipocyte regulation by downregulating PPARγ protein, palmitoleic acid not only increased the transdifferentiation of bovine satellite cells into adipocytes by PPARγ but also upregulated PLIN1 proteins [70,71]. This is the only study to analyze the effect on PLIN1 protein expression.…”

mentioning

confidence: 95%

PPARγ Gene as a Possible Link between Acquired and Congenital Lipodystrophy and its Modulation by Dietary Fatty Acids

et al. 2022

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Lipodystrophy syndromes are rare diseases that could be of genetic or acquired origin. The main complication of lipodystrophy is the dysfunction of adipose tissue, which leads to an ectopic accumulation of triglycerides in tissues such as the liver, pancreas and skeletal muscle. This abnormal fat distribution is associated with hypertriglyceridemia, insulin resistance, liver steatosis, cardiomyopathies and chronic inflammation. Although the origin of acquired lipodystrophies remains unclear, patients show alterations in genes related to genetic lipodystrophy, suggesting that this disease could be improved or aggravated by orchestrating gene activity, for example by diet. Nowadays, the main reason for adipose tissue dysfunction is an imbalance in metabolism, caused in other pathologies associated with adipose tissue dysfunction by high-fat diets. However, not all dietary fats have the same health implications. Therefore, this article aims to summarize the main genes involved in the pathophysiology of lipodystrophy, identify connections between them and provide a systematic review of studies published between January 2017 and January 2022 of the dietary fats that can modulate the development of lipodystrophy through transcriptional regulation or the regulation of protein expression in adipocytes.

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Effect of palmitoleic acid on the differentiation of bovine skeletal muscle satellite cells

Abstract: Competing interestsNo potential conflict of interest relevant to this article was reported. Funding sourcesState funding sources (grants, funding sources, equipment, and supplies). Include name and number of grant if available.

Cited by 8 publications

References 48 publications

Overexpression of DGAT2 Stimulates Lipid Droplet Formation and Triacylglycerol Accumulation in Bovine Satellite Cells

Overexpression of DGAT2 Stimulates Lipid Droplet Formation and Triacylglycerol Accumulation in Bovine Satellite Cells

— Invited Review — Translational gut microbiome research for strategies to improve beef cattle production sustainability and meat quality

PPARγ Gene as a Possible Link between Acquired and Congenital Lipodystrophy and its Modulation by Dietary Fatty Acids

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