Effect of P stoichiometry on the abundance of nitrogen-cycle genes in phosphorus-limited paddy soil

Wei, Xiaomeng; Hu, Yajun; Peng, Ping; Zhu, Zhenke; Atere, Cornelius Talade; O’Donnell, Anthony G.; Wu, Jinshui; Ge, Tida

doi:10.1007/s00374-017-1221-1

Cited by 99 publications

(21 citation statements)

References 54 publications

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“…Rice production on terraces requires large amounts of N fertilizer and comprises a substantial amount of China's rice harvest. Paddy soils are characterized by constantly alternation of wetting and drying, which will lead to great changes in soil properties and oxygen content (Atere et al, 2018), and influence the activity of nitrifiers compared with upland soils (Hu et al, 2013;Wei et al, 2017). DNA-SIP, as a powerful technique, can directly link the active lineages with defined activities in the complex soil environment.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Nitrosospira cluster 3-like bacterial ammonia oxidizers and Nitrospira-like nitrite oxidizers dominate nitrification activity in acidic terrace paddy soils

Zhang

et al. 2019

Soil Biology and Biochemistry

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The isolation of acid-adapted ammonia-oxidizing bacteria (AOB) has suggested the functional importance of AOB in acidic soils. However, there is, currently, no convincing evidence that links AOB activity to nitrification in acidic paddy soils. Here we demonstrated the incorporation of 13 CO 2 into the genomes of ammonia-oxidizing archaea (AOA), AOB and nitrite-oxidizing bacteria (NOB) following urea application by using stable isotope probing (SIP) in three acidic terrace paddy soils with altitudes of 200 m (E200), 600 m (E600) and 1100 m (E1100), respectively. Nitrification activity increased from E200 to E600 and then to E1100, accompanied with significant growth of AOB over the 56-day incubation, while the abundance of archaeal amoA gene declined significantly in all soils after incubation. DNA-SIP demonstrated that active AOB outnumbered AOA and were much more heavily labeled than AOA and NOB, implying their more significant contributions to nitrification in these soils. Phylogenetic analysis indicated that Nitrosospira cluster 3-like AOB predominantly catalyzed bacterial ammonia oxidation. 13 C-labeled NOB was dominated by Nitrospira moscoviensis in E1100, while in E200 and E600, Nitrospira marina and Nitrospira japonica were as prevalent as Nitrospira moscoviensis, respectively. Canonical correlation analysis and the Mantel test indicated the importance of soil physiochemical properties (e.g., pH, available phosphorus (AP) and soil oxidation capacity (OXC)) in determining the composition of the active nitrifying populations. These results suggest a greater functional importance of AOB in ammonia oxidation in the tested acidic paddy soils and the

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Section: Accepted Manuscriptmentioning

confidence: 99%

Nitrosospira cluster 3-like bacterial ammonia oxidizers and Nitrospira-like nitrite oxidizers dominate nitrification activity in acidic terrace paddy soils

Zhang

et al. 2019

Soil Biology and Biochemistry

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“…AOA which exhibit the capacity of detoxification of reactive oxygen species (ROS) may support them to be active in the microaerobic environment [15]. amoA-based quantification showed that AOA was more abundant in the paddy soils [16] [17]. In consideration of those data, it seems that AOA might be more active to the paddy soils rather than AOB.…”

Section: Introductionmentioning

confidence: 99%

Compositional Shifts in Ammonia-Oxidizing Microorganism Communities of Eight Geographically Different Paddy Soils —Biogeographical Distribution of Ammonia-Oxidizing Microorganisms

Lu¹,

Li²,

He³

et al. 2018

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Soil nitrification is mediated by ammonia-oxidizing archaea (AOA) and bacteria (AOB), which occupy different specialized ecological niches. However, little is known about the diversification of AOA and AOB communities in a large geographical scale. Here, eight paddy soils collected from different geographic regions in China were selected to investigate the spatial distribution of AOA and AOB, and their potential nitrification activity (PNA). The result showed that the abundance of AOA was predominant over AOB, indicating that the rice fields favor the growth of AOA. PNA highly varied from 0.43 to 3.57 μg NO X-N•g•dry•soil•h −1 , and was positively related with soil NH 3 content, the abundance of AOA community, and negatively related with the diversity of AOB community (P < 0.01), which indicating that AOA might be the more dominant ammonia oxidizers in the collected paddy soils. Denaturing gradient gel electrophoresis fingerprints of amoA genes revealed remarkable differences in the compositions of AOA and AOB community. Phylogenetic analyses of amoA genes showed that Nitrosospiracluster-3-like and Nitrosomonas cluster 7-like AOB extensively dominated the AOB communities, and 54d9-like AOA within the soil group 1.1b predominated in AOA communities in paddy soils. Redundancy analysis suggested that the spatial variations of AOA community structure were influenced by soil TN content (P < 0.01), while no significant correlation between AOB community structure and soil properties was found. Findings highlight that ammonia oxidizers exhibit spatial variations in complex paddy fields due to the joint influence of soil

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“…Phosphorus application decreased the soil N:P ratios, which aggravated the competition for N between the plant community and AOB (Hu et al, 2016), and thus increased the N limitation for some AOB taxa and decreased AOB diversity. Phosphorus addition may have reduced the unfavourable niches for AOB and thus in turn decreased AOB abundance in the Tibetan alpine steppe soil of the present study (Tang et al, 2016;Wei et al, 2017).…”

Section: Effects Of P Additionmentioning

confidence: 61%

“…Wei et al (2017) found the change in soil C:N:P stoichiometry after P addition could trigger competition among AOA, AOB and other soil microbes. Generally, P addition directly affects cellular growth rates of ammoniaoxidizers and thereby indirectly regulates the soil Ncycling pathway (Godwin & Cotner, 2015;Tang et al, 2016;Wei et al, 2017). However, the environmental factors that shape AOA and AOB communities in soils have not yet been established.…”

mentioning

confidence: 96%

“…Phosphorus application usually affects plant and microbial communities through its interaction with soil N (Lage et al, 2010;Xiang et al, 2017). Wei et al (2017) found the change in soil C:N:P stoichiometry after P addition could trigger competition among AOA, AOB and other soil microbes. Generally, P addition directly affects cellular growth rates of ammoniaoxidizers and thereby indirectly regulates the soil Ncycling pathway (Godwin & Cotner, 2015;Tang et al, 2016;Wei et al, 2017).…”

mentioning

confidence: 99%

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Responses of ammonia‐oxidizing archaea and bacteria to nitrogen and phosphorus amendments in an alpine steppe

Dong

Che

Jia³

et al. 2019

European J Soil Science

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With the increasing demands of livestock production, grasslands are under pressure from over‐intensified grazing. Phosphorus (P) and nitrogen (N) fertilization is widely employed to meet the nutrient demands of heavy grazing. Although soil ammonia‐oxidizers play a critical role in determining N dynamics after fertilizer application, their responses to fertilization are still not well understood in steppe grassland systems. Here, the individual and combined effects of N (0, 7.5 and 15 g N m−2 year−1) and P (0, 3.27, 6.55 and 13.09 g P m−2 year−1) applications on soil ammonia‐oxidizers were explored in the Tibetan alpine steppe. Ammonia‐oxidizing archaea (AOA) and bacteria (AOB) abundance and community composition were examined using qPCR, terminal restriction fragment length polymorphism and clone libraries. Results showed that AOB diversity was significantly increased by N fertilization and decreased by P fertilization. The abundance of AOB was significantly increased by N fertilization and its interactive effects with P application. In contrast, AOA community diversity and abundance remained unaffected by either N or P application. The AOB abundance and diversity were affected by the direct effects of N fertilization, as well as its indirect effects of N application through available N and ammonium (NH4+), and further analysis showed that NH4+ and pH were the main factors. The changes to the ammonia‐oxidizing community altered the total N content of plants and the N:P ratio of Gramineae. Plant P properties were influenced by P addition, which also interacted with soil pH and available N to indirectly affect AOB. Overall, AOB exhibited stronger responses to N and P fertilization of alpine steppe grassland than AOA, and appear to play a critical role in plant nutrient absorption under fertilization management. Highlights Effects of N and P application on AOA and AOB communities were evaluated. AOB, not AOA, responded to N or P, and their interaction, and NH4+ and pH were regulators of AOB. N application affected AOB community, resulting in changes to plant nutrient absorption. P addition affected plant tissue stoichiometry, and thus affected AOB community.

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Effect of P stoichiometry on the abundance of nitrogen-cycle genes in phosphorus-limited paddy soil

Cited by 99 publications

References 54 publications

Nitrosospira cluster 3-like bacterial ammonia oxidizers and Nitrospira-like nitrite oxidizers dominate nitrification activity in acidic terrace paddy soils

Nitrosospira cluster 3-like bacterial ammonia oxidizers and Nitrospira-like nitrite oxidizers dominate nitrification activity in acidic terrace paddy soils

Compositional Shifts in Ammonia-Oxidizing Microorganism Communities of Eight Geographically Different Paddy Soils —Biogeographical Distribution of Ammonia-Oxidizing Microorganisms

Responses of ammonia‐oxidizing archaea and bacteria to nitrogen and phosphorus amendments in an alpine steppe

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