Effect of overexpression of SNF1 on the transcriptional and metabolic landscape of baker’s yeast under freezing stress

Lü, Meng Kai; Xu, Yang; Lin, Xue; Jiang, Huan-Yuan; Hu, Xiaoping; Liu, Sixin

doi:10.1186/s12934-020-01503-0

Cited by 18 publications

(11 citation statements)

References 52 publications

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“…The serine/threonine protein kinase ( SNF1 expressed) is an important regulator of yeast in response to stress. Overexpression of SNF1 is effective in enhancing the cell tolerance and fermentation capacity of baker’s yeast in freezing, which may be related to the upregulated proteasome, altered metabolism of carbon sources and protectant molecules, and changed cell membrane components [ 38 , 39 ]. Ycp4p is predicted to be palmitoylated, a posttranslational modification typical of membrane-binding proteins involved in signal transduction.…”

Section: Discussionmentioning

confidence: 99%

Deletion of NTH1 and HSP12 increases the freeze–thaw resistance of baker’s yeast in bread dough

Chen

Lin

2022

Microb Cell Fact

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Background The intracellular molecule trehalose in Saccharomyces cerevisiae may have a major protective function under extreme environmental conditions. NTH1 is one gene which expresses trehalase to degrade trehalose. Small heat shock protein 12 (HSP12 expressed) plays a role in protecting membranes and enhancing freezing stress tolerance. Results An optimized S. cerevisiae CRISPR-Cpf1 genome-editing system was constructed. Multiplex genome editing using a single crRNA array was shown to be functional. NTH1 or/and HSP12 knockout in S. cerevisiae enhanced the freezing stress tolerance and improved the leavening ability after freezing and thawing. Conclusions Deleting NTH1 in the combination with deleting HSP12 would strengthen the freezing tolerance and protect the cell viability from high rates of death in longer-term freezing. It provides valuable insights for breeding novel S. cerevisiae strains for the baking industry through a more precise, speedy, and economic genome-editing system.

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Section: Discussionmentioning

confidence: 99%

Deletion of NTH1 and HSP12 increases the freeze–thaw resistance of baker’s yeast in bread dough

Chen

Lin

2022

Microb Cell Fact

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“…Although its structure is well known, research on its various cellular regulatory mechanisms and their potential applications is ongoing. For example, the SNF1 complex has been applied to improve yeast cell resistance to various environmental stresses, improve lipid accumulation, improve leavening abilities, and so on [ 24 , 26 , 29 ]. In this study, the multiple effects of REG1 deletion combined with SNF1 overexpression were used to improve SAM production in S. cerevisiae , which provided a reference for the application of the SNF1 complex to abolish glucose repression and redirect carbon flux to nonethanol products in S. cerevisiae .…”

Section: Discussionmentioning

confidence: 99%

“…1 b) [ 23 , 27 ]. It was also reported that the overexpression of the SNF1 gene enhanced the responses to various environmental stresses, and the overexpression of SNF4 and the deletion of GLC7 or REG1 effectively enhanced maltose metabolism and leavening ability by ameliorating glucose repression in yeast [ 24 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

The multiple effects of REG1 deletion and SNF1 overexpression improved the production of S-adenosyl-l-methionine in Saccharomyces cerevisiae

et al. 2022

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Background Saccharomyces cerevisiae is often used as a cell factory for the production of S-adenosyl-l-methionine (SAM) for diverse pharmaceutical applications. However, SAM production by S. cerevisiae is negatively influenced by glucose repression, which is regulated by a serine/threonine kinase SNF1 complex. Here, a strategy of alleviating glucose repression by deleting REG1 (encodes the regulatory subunit of protein phosphatase 1) and overexpressing SNF1 (encodes the catalytic subunit of the SNF1 complex) was applied to improve SAM production in S. cerevisiae. SAM production, growth conditions, glucose consumption, ethanol accumulation, lifespan, glycolysis and amino acid metabolism were analyzed in the mutant strains. Results The results showed that the multiple effects of REG1 deletion and/or SNF1 overexpression exhibited a great potential for improving the SAM production in yeast. Enhanced the expression levels of genes involved in glucose transport and glycolysis, which improved the glucose utilization and then elevated the levels of glycolytic intermediates. The expression levels of ACS1 (encoding acetyl-CoA synthase I) and ALD6 (encoding aldehyde dehydrogenase), and the activity of alcohol dehydrogenase II (ADH2) were enhanced especially in the presence of excessive glucose levels, which probably promoted the conversion of ethanol in fermentation broth into acetyl-CoA. The gene expressions involved in sulfur-containing amino acids were also enhanced for the precursor amino acid biosynthesis. In addition, the lifespan of yeast was extended by REG1 deletion and/or SNF1 overexpression. As expected, the final SAM yield of the mutant YREG1ΔPSNF1 reached 8.28 g/L in a 10-L fermenter, which was 51.6% higher than the yield of the parent strain S. cerevisiae CGMCC 2842. Conclusion This study showed that the multiple effects of REG1 deletion and SNF1 overexpression improved SAM production in S. cerevisiae, providing new insight into the application of the SNF1 complex to abolish glucose repression and redirect carbon flux to nonethanol products in S. cerevisiae.

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“…Additionally, in yeast the ATG autophagy pathway may collaborate with the SNF1 pathway to enhance survival under adverse environmental conditions, and inhibition of SNF1 would likely induce fungal degeneration over time [20]. Moreover, a contribution of Snf1 to yeast cell tolerance to freezing was also demonstrated [56].…”

Section: Other Roles Of the Snf1 Complex In Yeast And Filamentous Fungimentioning

confidence: 99%

Snf1 Kinase Differentially Regulates Botrytis cinerea Pathogenicity according to the Plant Host

et al. 2022

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The Snf1 kinase of the glucose signaling pathway controls the response to nutritional and environmental stresses. In phytopathogenic fungi, Snf1 acts as a global activator of plant cell wall degrading enzymes that are major virulence factors for plant colonization. To characterize its role in the virulence of the necrotrophic fungus Botrytis cinerea, two independent deletion mutants of the Bcsnf1 gene were obtained and analyzed. Virulence of the Δsnf1 mutants was reduced by 59% on a host with acidic pH (apple fruit) and up to 89% on hosts with neutral pH (cucumber cotyledon and French bean leaf). In vitro, Δsnf1 mutants grew slower than the wild type strain at both pH 5 and 7, with a reduction of 20–80% in simple sugars, polysaccharides, and lipidic carbon sources, and these defects were amplified at pH 7. A two-fold reduction in secretion of xylanase activities was observed consequently to the Bcsnf1 gene deletion. Moreover, Δsnf1 mutants were altered in their ability to control ambient pH. Finally, Δsnf1 mutants were impaired in asexual sporulation and did not produce macroconidia. These results confirm the importance of BcSnf1 in pathogenicity, nutrition, and conidiation, and suggest a role in pH regulation for this global regulator in filamentous fungi.

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Effect of overexpression of SNF1 on the transcriptional and metabolic landscape of baker’s yeast under freezing stress

Cited by 18 publications

References 52 publications

Deletion of NTH1 and HSP12 increases the freeze–thaw resistance of baker’s yeast in bread dough

Deletion of NTH1 and HSP12 increases the freeze–thaw resistance of baker’s yeast in bread dough

The multiple effects of REG1 deletion and SNF1 overexpression improved the production of S-adenosyl-l-methionine in Saccharomyces cerevisiae

Snf1 Kinase Differentially Regulates Botrytis cinerea Pathogenicity according to the Plant Host

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