Effect of Ovary Lipid of Skipjack Tuna (Katsuwonus pelamis) on Anxious Behavior in Rats

Abstract

ABSTRACT. Ovary lipid of Skipjack tuna (OLS) (Katsuwonus pelamis) contains a high level of docosahexaenoic acid combined with phospholipids. In this study, we examined the effect of OLS in male Wistar rats given OLS mixed in feed (0.9%) for 42 days, using an animal model of anxiety, the elevated T-maze test. The avoidance latency at the 1st trial was significantly shorter in the OLS ingestion group than in the control group. Those at the 2nd and 3rd trials showed a similar tendency. There was almost no difference in escape latency at the 1st trial between the two groups but the escape latencies at the 2nd and 3rd trials tended to be longer in the OLS group. These results suggested that OLS inhibits anxious behavior in rats. KEY WORDS: anxiety, elevated T-maze test, ovary lipid.

Effect of Ovary Lipid from Skipjack Tuna (Katsuwonus pelamis) on Brain Monoamines in Rats

“…The same is true of the serum components TG and NEFA. In addition, the HDL concentration was lower in the OLS groups, as reported previously [14,15]. Although no signif-icant difference test was performed because an interaction of the stress and OLS factors was observed, the OLS group under chronic stress, compared with the control group, tended to have a lower CORT level.…”

“…In previous experiments with rats, we found that OLS inhibited the increase in corticosterone (CORT) production under acute stress, stimulated the secretion of dehydroepiandrosterone (often referred to as the antistress hormone) [1,6], and accelerated the decrease in CORT levels following relief from stress [14]. In addition, elevated T-maze tests [5,7,16,21] demonstrated that OLS had the ability to mitigate anxiety and/or fear [15].…”

Anti-anxiety Effect of Ovary Lipid Extracted from Skipjack Tuna (Katsuwonus pelamis) in Rats

“…Previously, we studied anxiety and/or fear using an elevated T-maze test with rats given OLS. We found that OLS shortened avoidance latency at the first trial, indicating that it has an effect against anxiety and/or fear [9]. In the present study, we evaluated the anti-anxiety effect of OLS using an elevated plus-maze test [1, 3, 4, 6-8, 12, 15], which is often used to determine anxiety level.…”

“…As OLS is rich in n-3 fatty acids, the control feed was prepared to give the same energy percentage as n-3 fatty acids and the same n-6/n-3 ratio [9]. In the present study, rats were also given OLS mixed as a lipid component in feed.…”