Effect of Ovariectomy on Renal Estrogen Receptor-α and Estrogen Receptor-β in Young Salt-Sensitive and -Resistant Rats

Esqueda, Maria Eugenia Davila; Craig, Teresa; Hinojosa‐Laborde, Carmen

doi:10.1161/hypertensionaha.107.095265

Cited by 34 publications

(26 citation statements)

References 33 publications

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“…Consistent with our observations, Catanuto et al [31] have reported ERβ expression in the kidney. In a recent study in female rats, ERβ was detected in both the cortex and medulla [32]. The present studies revealed that ERβ was similarly expressed in the mesangial cells and vasculature of males and females.…”

Section: Discussionsupporting

confidence: 71%

Classical Estrogen Receptors and ERα Splice Variants in the Mouse

2013

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Estrogens exert a variety of effects in both reproductive and non-reproductive tissues. With the discovery of ERα splice variants, prior assumptions concerning tissue-specific estrogen signaling need to be re-evaluated. Accordingly, we sought to determine the expression of the classical estrogen receptors and ERα splice variants across reproductive and non-reproductive tissues of male and female mice. Western blotting revealed that the full-length ERα66 was mainly present in female reproductive tissues but was also found in non-reproductive tissues at lower levels. ERα46 was most highly expressed in the heart of both sexes. ERα36 was highly expressed in the kidneys and liver of female mice but not in the kidneys of males. ERβ was most abundant in non-reproductive tissues and in the ovaries. Because the kidney has been reported to be the most estrogenic non-reproductive organ, we sought to elucidate ER renal expression and localization. Immunofluorescence studies revealed ERα66 in the vasculature and the glomerulus. It was also found in the brush border of the proximal tubule and in the cortical collecting duct of female mice. ERα36 was evident in mesangial cells and tubular epithelial cells of both sexes, as well as podocytes of females but not males. ERβ was found primarily in the podocytes in female mice but was also present in the mesangial cells in both sexes. Within the renal cortex, ERα46 and ERα36 were mainly located in the membrane fraction although they were also present in the cytosolic fraction. Given the variability of expression patterns demonstrated herein, identification of the specific estrogen receptors expressed in a tissue is necessary for interpreting estrogenic effects. As this study revealed expression of the ERα splice variants at multiple sites within the kidney, further studies are warranted in order to elucidate the contribution of these receptors to renal estrogen responsiveness.

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Section: Discussionsupporting

confidence: 71%

Classical Estrogen Receptors and ERα Splice Variants in the Mouse

2013

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“…There are many papers already published showing the expression of estrogen receptor in proximal tubules of rat, mouse and opossum kidney cells. These articles showed that the kidneys have the expression of nuclear estrogen receptor (ER-a and ER-ß) and transmembrane G-protein couple estrogen receptor (GPR30) [41][42][43][44][45].…”

Section: Discussionmentioning

confidence: 99%

Role of Estrogen and Progesterone in the Modulation of CNG-A1 and Na⁺/K⁺-ATPase Expression in the Renal Cortex

Gracelli

Souza-Menezes²,

Barbosa³

et al. 2012

Cell Physiol Biochem

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The steroid hormones, estrogen and progesterone, are involved mainly in the control of female reproductive functions. Among other effects, estrogen and progesterone can modulate Na⁺ reabsorption along the nephron altering the body’s hydroelectrolyte balance. In this work, we analyzed the expression of cyclic nucleotide-gated channel A1 (CNG-A1) and α1 Na⁺/K⁺-ATPase subunit in the renal cortex and medulla of female ovariectomized rats and female ovariectomized rats subjected to 10 days of 17β-estradiol benzoate (2.0 µg/kg body weight) and progesterone (1.7 mg/kg body weight) replacement. Na⁺/K⁺ ATPase activity was also measured. Immunofluorescence localization of CNG-A1 in the cortex and medulla was performed in control animals. We observed that CNG-A1 is localized at the basolateral membrane of proximal and distal tubules. Female ovariectomized rats showed low expression of CNG-A1 and low expression and activity of Na⁺/K⁺ ATPase in the renal cortex. When female ovariectomized rats were subjected to 17β-estradiol benzoate replacement, normalization of CNG-A1 expression and Na⁺/K⁺ ATPase expression and activity was observed. The replacement of progesterone was not able to recover CNG-A1 expression and Na⁺/K⁺ ATPase expression at the control level. Only the activity of Na⁺/K⁺ ATPase was able to be recovered at control levels in animals subjected to progesterone replacement. No changes in expression and activity were observed in the renal medulla. The expression of CNG-A1 is higher in cortex compared to medulla. In this work, we observed that estrogen and progesterone act in renal tissues modulating CNG-A1 and Na⁺/K⁺ ATPase and these effects could be important in Na⁺ and water balance.

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“…The lack of a consistent decrease in plasma estrogen levels as a result of ovariectomy was unexpected since the ovary is a major glandular source of circulating estrogen. Ovariectomy in the rat has previously been reported to reduce plasma estradiol levels by only 30-50% (Alagwu and Neli, 2005;Esqueda et al, 2007). Hence, the remainder is derived from the conversion of circulating adrenal androstenedione in adipose tissue by aromatisation and reduction (Grodin et al, 1973).…”

Section: Discussionmentioning

confidence: 99%

Dietary phytoestrogens maintain contractile responses to carbachol with age in the female rat isolated bladder

Owen¹,

Rose’Meyer²,

Massa³

2011

Life Sciences

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Effect of Ovariectomy on Renal Estrogen Receptor-α and Estrogen Receptor-β in Young Salt-Sensitive and -Resistant Rats

Cited by 34 publications

References 33 publications

Classical Estrogen Receptors and ERα Splice Variants in the Mouse

Classical Estrogen Receptors and ERα Splice Variants in the Mouse

Role of Estrogen and Progesterone in the Modulation of CNG-A1 and Na⁺/K⁺-ATPase Expression in the Renal Cortex

Dietary phytoestrogens maintain contractile responses to carbachol with age in the female rat isolated bladder

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Effect of Ovariectomy on Renal Estrogen Receptor-α and Estrogen Receptor-β in Young Salt-Sensitive and -Resistant Rats

Cited by 34 publications

References 33 publications

Classical Estrogen Receptors and ERα Splice Variants in the Mouse

Classical Estrogen Receptors and ERα Splice Variants in the Mouse

Role of Estrogen and Progesterone in the Modulation of CNG-A1 and Na+/K+-ATPase Expression in the Renal Cortex

Dietary phytoestrogens maintain contractile responses to carbachol with age in the female rat isolated bladder

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Role of Estrogen and Progesterone in the Modulation of CNG-A1 and Na⁺/K⁺-ATPase Expression in the Renal Cortex