Dietary phytoestrogens maintain contractile responses to carbachol with age in the female rat isolated bladder

Owen, Suzzanne; Rose’Meyer, Roselyn Barbara; Massa, Helen Maureen

doi:10.1016/j.lfs.2011.05.022

Cited by 8 publications

(9 citation statements)

References 41 publications

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“…In aged rats (52 weeks), plasma oestrogen levels were over 400% higher than those recorded from the young and mature rats, but did not show additional significant variability with either diet or ovariectomy treatments (P>0.05, data not shown; see Owen et al 2011).…”

Section: Resultsmentioning

confidence: 83%

“…Effect of ovariectomy or phytoestrogen diet on carbachol-induced contraction At 12 weeks of age, the potency or maximal contractile response to carbachol did not change as a result of dietary phytoestrogens or loss of oestrogen (data not shown, P> 0.05; see Owen et al 2011). However, at 24 weeks of age, carbachol had reduced potency in detrusor strips from nonsoy ovx treated rats when compared to age-matched non-soy sham (P<0.05).…”

Section: Resultsmentioning

confidence: 85%

“…Plasma progesterone concentrations, derived from ovarian sources, were significantly reduced (~30-50%) as a result of ovariectomy, regardless of diet, in both young and mature age groups (P<0.05, data not shown; see Owen et al 2011). …”

Section: Resultsmentioning

confidence: 88%

“…The commercial rat chow contains soy meal (meat free, rat and mouse, provided by Specialty Feeds, WA, USA). The constituents of the nonsoy diet are listed in Owen et al (2011). The commercially obtained rat chow contains ingredients including wheat, barley, soya meal, fish meal and mixed vegetable oils while the non-soy feed comprised casein purified whey protein, corn starch, corn or sunflower oil.…”

Section: Dietmentioning

confidence: 99%

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Loss of adenosine A2B receptor mediated relaxant responses in the aged female rat bladder; effects of dietary phytoestrogens

Owen

Massa

Rose’Meyer

2012

Naunyn-Schmiedeberg's Arch Pharmacol

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This study examined the effect of age, ovariectomy and dietary phytoestrogen ingestion on adenosine A(2B) receptor mediated relaxant responses and mRNA expression of adenosine receptor subtypes in the rat isolated bladder. Female Wistar rats (8 weeks) were anaesthetised and the ovaries were removed (ovx) or left intact (sham). Rats were fed either normal rat chow (soy, phytoestrogens) or a non-soy (phytoestrogen free) diet. Isolated bladder from rats aged 12, 24 or 52 weeks were pre-contracted with 3 μM carbachol prior to a concentration response curve to 5'-(N-ethylcarboxamido) adenosine (NECA) being obtained. In 12-week-old rats, the bladder exhibited enhanced relaxant responses to NECA in soy-fed rats (P < 0.05), whilst at 24 weeks of age, the relaxant responses to NECA were attenuated in all the groups studied except soy-treated sham rat bladders in which the relaxant responses were enhanced. At 52 weeks of age, no relaxant effects were observed in any of the treatment groups and NECA-induced contractile responses occurred. In all bladders, the adenosine A(2B) receptor was the most abundantly expressed. In bladders from young and mature female rats, the mRNA expression of adenosine receptors (A(1), A(2A) and A(2B)) was lowest in the bladder from non-soy-fed ovariectomised animals and the use of phytoestrogens in the diet increased the mRNA expression of these receptors (P < 0.05). While a soy diet improves the relaxant effects to the adenosine analogue via adenosine A(2B) receptors in bladders from younger rats, the benefits are lost with advancing age.

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Section: Resultsmentioning

confidence: 83%

Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 88%

Section: Dietmentioning

confidence: 99%

See 2 more Smart Citations

Loss of adenosine A2B receptor mediated relaxant responses in the aged female rat bladder; effects of dietary phytoestrogens

Owen

Massa

Rose’Meyer

2012

Naunyn-Schmiedeberg's Arch Pharmacol

Self Cite

View full text Add to dashboard Cite

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“…A long-term phytoestrogen diet in rats also mediated age-related reduced detrusor activity. [26] Similar to the 2008 study in cynomolgus monkeys, a 2011 study in ovariectomized versus sham operated Wistar rats showed that a soy diet was associated with increased bladder detrusor muscle contractile response in aged (52 week old) rats. [26] No change in bladder contractile response was seen in the young (12 week old) rats regardless of the surgery or soy diet.…”

Section: Resultsmentioning

confidence: 90%

A Review of Phytoestrogens and Their Association With Pelvic Floor Conditions

Cardenas-Trowers¹,

Meyer²,

Markland³

et al. 2018

Female Pelvic Med Reconstr Surg

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Although the literature is limited, most studies on phytoestrogens and PFDs support associations with pathophysiologic mechanisms, symptoms, and treatment for urinary incontinence and pelvic organ prolapse, but not fecal incontinence. Less is known regarding the prevention of PFDs with phytoestrogen intake over time. Overall, the potential influence of phytoestrogens on PFDs is not well understood, and more research is needed.

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Dietary phytoestrogen improves relaxant responses to 17-β-estradiol in aged but not ovariectomised rat bladders

Owen

Massa

Rose’Meyer

2013

Naunyn-Schmiedeberg's Arch Pharmacol

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This study examined the effect of age, ovariectomy and dietary phytoestrogen ingestion on 17-β-estradiol-mediated relaxant responses and messenger RNA (mRNA) and protein expression of oestrogen receptor subtypes in the rat isolated bladder. Female Wistar rats (8 weeks) were anaesthetised, and the ovaries were removed (ovx) or left intact (sham). Rats were fed either normal rat chow (soy, phytoestrogens) or a non-soy (phytoestrogen free) diet. Isolated bladder from rats aged 12, 24 or 52 weeks were pre-contracted with 3 μM carbachol prior to obtaining a concentration response curve to 17-β-estradiol. Protein and mRNA expression of the oestrogen receptor subtypes was completed using immunohistochemistry and real-time PCR, respectively. Relatively moderate relaxant responses to 17-β-estradiol were observed in bladders from all age and treatment groups. However, in soy-fed sham 52-week-old rats, the bladder exhibited enhanced relaxant responses to 17-β-estradiol when compared to tissues from other age-matched rat treatment groups (P < 0.05). In bladders from female rats, the mRNA and protein expression of oestrogen receptors β was significantly greater than the expression of the oestrogen receptor α. Oestrogen receptor α mRNA expression declined with age (P < 0.05), whereas oestrogen receptor β expression did not change in any of the treatment groups (P > 0.05). Diet, overiectomy or age did not alter the protein expression of either oestrogen receptor subtype in the bladder (P > 0.05). While a soy diet improved relaxant effects to the 17-β-estradiol with age, it did not alter relaxant responses in bladders from ovariectomised rats.

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Dietary phytoestrogens maintain contractile responses to carbachol with age in the female rat isolated bladder

Cited by 8 publications

References 41 publications

Loss of adenosine A2B receptor mediated relaxant responses in the aged female rat bladder; effects of dietary phytoestrogens

Loss of adenosine A2B receptor mediated relaxant responses in the aged female rat bladder; effects of dietary phytoestrogens

A Review of Phytoestrogens and Their Association With Pelvic Floor Conditions

Dietary phytoestrogen improves relaxant responses to 17-β-estradiol in aged but not ovariectomised rat bladders

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