Effect of ovariectomy followed by administration of ovarian steroid hormones on the pig pinealocyte ultrastructure

Przybylska, B; Wyrzykowski, Z; Kaleczyc, Jerzy

doi:10.1016/0039-128x(93)90004-7

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…Morphologically, the pineal gland of the pig is characterized by a large number of electron-dense bodies in pinealocyte (Karasek and Wyrzykowski, 1980;. The structure and number of the bodies change under various physiological (Wyrzykowski et al, 1987;Przybylska, 1990) and experimental (Przybylska et al, 1993 conditions.…”

Section: Tion; Pigmentioning

confidence: 99%

Demonstration of nerve fibers containing the C-terminal flanking peptide of neuropeptide Y (CPON) in the pig pineal gland (Sus domesticus): An immunocytochemical study by light and electron microscopy

Przybylska‐Gornowicz¹,

Lewczuk²,

Møller

1997

Anat. Rec.

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Background The pineal gland is richly innervated by the neuropeptide Y (NPY)‐positive nerves that mostly exibit immunoreactivity for the enzyme tyrosine hydroxylase, a marker of sympathetic nerve fibers. NPY is synthesized as a part of larger prepromolecule. The present study was undertaken to demonstrate that the posttranslational processing of preproNPY resulted in the presence of C‐terminal flanking peptide of NPY (CPON) in nerve fibers of the pig pineal gland. Methods An immunohistochemical anti‐CPON technique was done over mounted sections of perfusion‐paraformaldehyde‐fixed material. An immunocytochemical preembedding technique was done to study the CPON‐positive nerve terminals under electron microscopy. Results The pig pineal gland is densely innervated by CPON‐immunoreactive nerve fibers. These nerve fibers follow from the pineal capsule into the connective tissue septa and farther into the pineal parenchyma, where the varicose branches terminate between the pinealocytes. The fiber density was the highest in the peripheral and ventral parts of the gland. At the ultrastructural level, the CPON‐immunoreactive nerve terminals were found in the perivascular spaces and in the parenchyma. The terminals contained small vesicles (30‐40 nm in diameter), some of which showed an eccentrically located dense core. In addition, large clear vesicles (80‐100 nm in diameter) were present. Some of the CPON‐immunoreactive nerve terminals were found in close apposition to the pinealocyte cell membrane, making a synapticlike contact with the pinealocytes. Conclusions Our results show the presence of dense CPON‐IR innervation in the pig pineal gland. The ultrastructural localization of CPON‐IR nerve terminals shows that the peptide can be released to both perivascular and intercellular spaces. The functional role for this peptide in pig pineal gland is still an open question. Anat. Rec. 248:576‐582, 1997. © 1997 Wiley‐Liss, Inc.

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Section: Tion; Pigmentioning

confidence: 99%

Demonstration of nerve fibers containing the C-terminal flanking peptide of neuropeptide Y (CPON) in the pig pineal gland (Sus domesticus): An immunocytochemical study by light and electron microscopy

Przybylska‐Gornowicz¹,

Lewczuk²,

Møller

1997

Anat. Rec.

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“…Four subtypes [MBB-1 A, MBB-IB, MBB-lC, conglomerates (CON)] were distinguished among the dense bodies of type I in the present study. The structure and the number of the dense bodies change during development as well as under various physiological and experimental conditions [Przybylska, 1990;Przybylska et al, 1988Przybylska et al, , 1990Przybylska et al, , 1992Przybylska et al, , 1993Przybylska-Gomowicz et al, 1996;Wyrzykowski et al, 1990Wyrzykowski et al, , 1992Lewczuk et al, 19961. The results of our previous investigations led to the hypothesis that these structures are involved in some of secretory processes in pig pinealoc ytes.…”

Section: Introductionmentioning

confidence: 99%

Effects of sympathicolytic and sympathomimetic drugs on pineal ultrastructure in the domestic pig

Lewczuk

Przybylska‐Gornowicz

1997

Journal of Pineal Research

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The effects of a 4-day administration of two sympathicolytic (alpha-methyl-p-tyrosine, propranolol) and three sympathicomimetic (clorgyline, isoproterenol, dobutamine) drugs on the ultrastructure of the pineal gland in the domestic pig were examined. The morphological changes in the pinealocytes of the experimental groups mainly concerned cytoplasmic dense bodies, primary lysosomes, multivesicular bodies, dense core vesicles, and smooth endoplasmic reticulum. These structures seemed to be under direct or indirect control by the adrenergic nervous system. The relative volume of dense bodies in pinealocyte perikarya increased after administration of alpha-methyl-p-tyrosine as well as propranolol and decreased on treatment with clorgyline. The decrease in the relative volume of primary lysosomes in pinealocyte perikarya was noted in pigs treated with clorgyline and isoproterenol. The number of multivesicular bodies in pinealocyte perikarya decreased after administration of alpha-methyl-p-tyrosine. The numerical density of dense core vesicles in pinealocyte perikarya increased after treatment with clorgyline. Administration of isoproterenol and clorgyline resulted in the presence of wide cisterns of smooth endoplasmic reticulum. Narrow cisterns of this form of endoplasmic reticulum and lamellar whorls were observed after treatment with alpha-methyl-p-tyrosine in pinealocytes with large dense bodies. The presence of multilamellar structures was a characteristic feature of the pinealocytes of pigs treated with isoproterenol and dobutamine. Administration of alpha-methyl-p-tyrosine and clorgyline resulted in the appearance of pinealocytes which differed in number, size, and structure of dense bodies.

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Adrenergic regulation of cytoplasmic structures related to secretory processes in pig pinealocytes—an ultrastructural, quantitative study

Przybylska‐Gornowicz

Lewczuk

Ziółkowska

et al. 2017

Micron

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Effect of ovariectomy followed by administration of ovarian steroid hormones on the pig pinealocyte ultrastructure

Cited by 3 publications

References 30 publications

Demonstration of nerve fibers containing the C-terminal flanking peptide of neuropeptide Y (CPON) in the pig pineal gland (Sus domesticus): An immunocytochemical study by light and electron microscopy

Demonstration of nerve fibers containing the C-terminal flanking peptide of neuropeptide Y (CPON) in the pig pineal gland (Sus domesticus): An immunocytochemical study by light and electron microscopy

Effects of sympathicolytic and sympathomimetic drugs on pineal ultrastructure in the domestic pig

Adrenergic regulation of cytoplasmic structures related to secretory processes in pig pinealocytes—an ultrastructural, quantitative study

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