Effect of ovarian hormones on periodical changes in immune resistance associated with estrous cycle in the beagle bitch

Sugiura, Kikuya; Nishikawa, Mami; Ishiguro, Kayoko; Tajima, Tomoko; Inaba, Muneo; Torii, Ryuzo; Hatoya, Shingo; Wijewardana, Viskam; Kumagai, D.; Tamada, Hiromichi; Sawada, Tsutomu; Ikehara, Susumu; Inaba, Toshio

doi:10.1016/j.imbio.2004.09.003

Cited by 52 publications

(37 citation statements)

References 34 publications

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“…In contrast, our study showed that P 4 significantly inhibited expression of IFN-γ and tended to inhibit TNF-α. Our results are consistent with those of Sugiura et al [27] that P 4 caused significant inhibition of the IFN-γ expression of PBMCs in anestrus dogs in response to E. coli isolated from the uterus affected by pyometra. Recently, Miyaura and Iwata [20] found that P 4 directly inhibits the development of Th1 cells, which are the sources of IFN-γ and TNF-α in PBMCs.…”

supporting

confidence: 93%

“…In addition, Watson et al [30] reported that mares with elevated plasma concentrations of P 4 had decreased bactericidal activity of neutrophils in the uterus. Also, in anestrus dogs, P 4 caused significant inhibition of the proliferation of peripheral blood mononuclear cells (PBMcs) in response to E. coli isolated from the uterus affected by pyometra [27]. Progesterone inhibits the proliferation of peripheral blood lymphocytes activated by mitogens in humans [22], cows [3], sheep [16] and pigs [31].…”

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confidence: 99%

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Effect of Progesterone on the in vitro Response of Peripheral Blood Mononuclear Cells Stimulated by Escherichia coli in Mares

Maeda

Ohtsuka

Tomioka

et al. 2012

The Journal of Veterinary Medical Science

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ABSTRAcT. Escherichia coli(E. coli) isolated from the uterus of a Thoroughbred mare with bacterial endometritis was used to evaluate the effect of progesterone (P 4 ) on the immune response of mares. Peripheral blood mononuclear cells (PBMcs) were collected from 10 nonpregnant clinically healthy adult mares (range, 4-12 years) during diestrus, four Thoroughbreds and six Hokkaido native horses. Cell proliferation and expression of cytokine mRNA, including interferon (IFN)-γ, tumor necrosis factor (TNF)-α and interleukin (IL)-10, of PBMcs stimulated with E. coli and P 4 were examined in vitro. P 4 was shown to have significantly inhibited E. coli induced proliferation and expression of IFN-γ in PBMCs. These results indicate that P 4 inhibits the immune response to E. coli in mares. KEY WORDS: bacterial endometritis, cytokine, Escherichia coli, mare, progesterone.doi: 10.1292/jvms.11-0364; J. Vet. Med. Sci. 74(5): 629-632, 2012 Endometritis is the third most common medical condition in horses [28] that causes infertility and results in major losses in the equine breeding industry. To date, bacterial uterine infection has been reported to occur in 25-60% of barren mares [2,5,7] and Escherichia coli(E. coli) are most frequently associated with fertility problems in mares [1]. It was reported that the endometrium of the mare shows an enhanced ability to eliminate infection during the follicular phase compared with the luteal phase of the estrous cycle [14]. Previous studies suggest that progesterone (P 4 ) is closely associated with the incidence of bacterial endometritis and that P 4 dominated uterus exhibits reduced physical clearance of bacteria in the mare [10]. These findings suggest that P 4 significantly affects the incidence of endometritis.Inflammation is initiated and maintained by the emergence of a network of proinflammatory cytokines, such as interferon (IFN)-γ and tumor necrosis factor (TNF)-α, and anti-inflammatory cytokines, such as interleukin (IL)-10 [24]. Recently, Fumuso et al. [12,13] reported that mares susceptible to developing persistent endometritis have higher endometrial expression of proinflammatory cytokines and lower levels of the anti-inflammatory cytokines. In addition, Watson et al. [30] reported that mares with elevated plasma concentrations of P 4 had decreased bactericidal activity of neutrophils in the uterus. Also, in anestrus dogs, P 4 caused significant inhibition of the proliferation of peripheral blood mononuclear cells (PBMcs) in response to E. coli isolated from the uterus affected by pyometra [27]. Progesterone inhibits the proliferation of peripheral blood lymphocytes activated by mitogens in humans [22], cows [3], sheep [16] and pigs [31]. Progesterone treatment of mares has been associated with prolonged infection after intrauterine inoculation with bacteria [4,14]. These reports in different species indicate that not only the status of systemic immunity affected by P 4 was associated with bacterial growth in the uterus but also that the immunosuppressive effects of P...

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confidence: 93%

mentioning

confidence: 99%

Effect of Progesterone on the in vitro Response of Peripheral Blood Mononuclear Cells Stimulated by Escherichia coli in Mares

Maeda

Ohtsuka

Tomioka

et al. 2012

The Journal of Veterinary Medical Science

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“…Leukocyte inhibition in the progesterone-primed uterus often supports bacterial growth. All these effects are cumulative and each estrous cycle is exacerbating this uterine pathology (Sugiura et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Influence of some factors on the incidence of pyometra in the bitch

Antonov¹,

Atanasov²,

Fasulkov³

et al. 2015

BJVM

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The aim of the present study was to determine the influence of breed, age, season, mating, pregnancy, progestin contraceptive treatment and the onset time of the disease after the last heat on the incidence and epidemiology of canine pyometra in Bulgaria. The investigation included 217 bitches suffering from pyometra. We found that the disorder was most often observed in 7-year-old, mixed breed bitches, most frequently in September and 4 weeks after the heat.

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“…potentially altered susceptibility) of the development of this disease in bitches has remained open to question. Sugiura et al (2004) demonstrated the influence of ovarian hormones on antigen-specific immune responses, particularly secondary responses. In their research, oestrogen appeared to upregulate, while progesterone downregulated the activity of lymphocytes in vivo and in vitro, effects which were also dependent on the stage of the oestrous cycle.…”

mentioning

confidence: 99%

The effects of in vitro exposure to progesterone and estradiol-17β on the activity of canine neutrophils

Bartošková¹,

Ondráčková²,

Levá³

et al. 2014

Vet. Med. - Czech

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ABSTRACT:To date, only limited information about the influence of ovarian hormones on canine immune system cells has been available. The study investigated the in vitro influence of progesterone and estradiol-17β on the activity of canine neutrophils. Treatment of cells by both hormones led to a significant decrease in phagocytosisinduced oxidative burst as detected using luminometry after stimulation with opsonised zymosan. The increase in oxidative burst, not connected with phagocytosis, was recorded after stimulation with a soluble stimulator. Using flow cytometry, the tendency of both hormones to decrease the production of reactive oxygen species associated with phagocytosis of Escherichia coli was also evident, although not significant. Suppression of canine neutrophil activity is not connected with pathogen recognition capabilities, since the expression of Toll-like receptor 4 was unaffected. This study reveals that both hormones have a suppressive effect on the activity of canine neutrophils and thus might contribute to the aetiology of pyometra.

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Effect of ovarian hormones on periodical changes in immune resistance associated with estrous cycle in the beagle bitch

Cited by 52 publications

References 34 publications

Effect of Progesterone on the <i>in vitro</i> Response of Peripheral Blood Mononuclear Cells Stimulated by <i>Escherichia coli</i> in Mares

Effect of Progesterone on the <i>in vitro</i> Response of Peripheral Blood Mononuclear Cells Stimulated by <i>Escherichia coli</i> in Mares

Influence of some factors on the incidence of pyometra in the bitch

The effects of in vitro exposure to progesterone and estradiol-17β on the activity of canine neutrophils

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