The effects of in vitro exposure to progesterone and estradiol-17β on the activity of canine neutrophils

Abstract

ABSTRACT:To date, only limited information about the influence of ovarian hormones on canine immune system cells has been available. The study investigated the in vitro influence of progesterone and estradiol-17β on the activity of canine neutrophils. Treatment of cells by both hormones led to a significant decrease in phagocytosisinduced oxidative burst as detected using luminometry after stimulation with opsonised zymosan. The increase in oxidative burst, not connected with phagocytosis, was recorded after stimulation with a soluble stimulator. Using flow cytometry, the tendency of both hormones to decrease the production of reactive oxygen species associated with phagocytosis of Escherichia coli was also evident, although not significant. Suppression of canine neutrophil activity is not connected with pathogen recognition capabilities, since the expression of Toll-like receptor 4 was unaffected. This study reveals that both hormones have a suppressive effect on the activity of canine neutrophils and thus might contribute to the aetiology of pyometra.

Polish Journal of Veterinary Sciences

“…) (Marin et al 2010, Bartoskova et al 2014. These observations seem to confirm the research of Doucet et al (2010), who showed a higher activity of neutrophil oxidative burst in rats after ovariectomy than in animals with preserved gonads.…”

Contribution of sex steroids and prolactin to the modulation of T and B cells during autoimmunity

“…and oxidative burst [138]. In humans, some authors have shown that mPRα expressed in peripheral blood T cells during pregnancy, is mainly involved in immune response regulation, generates fetal protection and impairs fetal complications [139].…”

Pregnancy and spontaneous partial abortion associated with salmonella in a dog

“…23 Similarly, this is speculated to occur in pregnant dogs, with steroid hormones suppressing neutrophilic activity. 24 Client education is important to implement hygiene protocols in the household and prevent zoonosis in pets with known infections. Best practices include wearing gloves, frequent hand washing, and isolation from those in the household.…”