Effect of osteocalcin deficiency on the nanomechanics and chemistry of mouse bones

Kavukcuoglu, N.B.; Patterson-Buckendahl, Patricia; Mann, A.

doi:10.1016/j.jmbbm.2008.10.010

Cited by 70 publications

(44 citation statements)

References 32 publications

(50 reference statements)

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“…47 There are a few studies of the correlation between biomechanical parameters and Raman spectroscopic composition parameters in mouse models of disorders, including osteogenesis imperfect (OI) [48][49][50][51] and osteoporosis, 52 as well as on the effects of knocking out matrix proteinase, 53 transcription factor 54 and noncollagenous proteins. [55][56][57][58][59][60][61][62] These are discussed in the following sections.…”

Section: Animal Age Effectsmentioning

confidence: 99%

“…Raman spectroscopic studies on the OC À / À genotype suggest that OC also has a role in mineralization, but unlike in the OPN À / À genotype mineral/matrix ratios were not different between OC À / À and OC þ / þ mice. 57 In contrast, OC À / À mice have lower carbonate/phosphate ratios and reduced crystallinity than OC þ / þ mice. This suggests that OC regulates the growth of apatitic crystals, which was consistent with nanoindentation studies in which tissue hardness increased more than elastic modulus in OC À / À mice.…”

Section: Fbn2mentioning

confidence: 99%

“…Femurs from fibrillin-2 (Fbn2 À / À ), 55 fetuin-A/alpha-HSglycoprotein (Ahsg À / À ), 59 osteopontin (OPN À / À ) 56 and osteocalcin (OC À / À ) 57 -deficient mice have been studied using bone mechanics and Raman spectroscopy. In one study,…”

mentioning

confidence: 99%

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Contributions of Raman spectroscopy to the understanding of bone strength

Mandair¹,

Morris²

2015

BoneKEy Reports

284

314

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Raman spectroscopy is increasingly commonly used to understand how changes in bone composition and structure influence tissue-level bone mechanical properties. The spectroscopic technique provides information on bone mineral and matrix collagen components and on the effects of various matrix proteins on bone material properties as well. The Raman spectrum of bone not only contains information on bone mineral crystallinity that is related to bone hardness but also provides information on the orientation of mineral crystallites with respect to the collagen fibril axis. Indirect information on collagen cross-links is also available and will be discussed. After a short introduction to bone Raman spectroscopic parameters and collection methodologies, advances in in vivo Raman spectroscopic measurements for animal and human subject studies will be reviewed. A discussion on the effects of aging, osteogenesis imperfecta, osteoporosis and therapeutic agents on bone composition and mechanical properties will be highlighted, including genetic mouse models in which structure-function and exercise effects are explored. Similarly, extracellular matrix proteins, proteases and transcriptional proteins implicated in the regulation of bone material properties will be reviewed.

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Section: Animal Age Effectsmentioning

confidence: 99%

Section: Fbn2mentioning

confidence: 99%

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Contributions of Raman spectroscopy to the understanding of bone strength

Mandair¹,

Morris²

2015

BoneKEy Reports

284

314

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“…Mechanical studies show young OPNÀ/À mice have lower hardness and elastic modulus compared with OPN +/+ mice, confirming osteopontin's role in the early stages of mineralization. Raman measurements on femoral bones from fibrillin 2 (Fbn2À/À) and (OCÀ/À) knockout mice have also been conducted and correlated with mechanical measurements [36]. For example, substantial intrabone variation in mechanics and crystallinity were been reported, especially in the midcortical section of OCÀ/À mice when compared with OC +/+ controls.…”

Section: Mouse Models Of Bone Propertiesmentioning

confidence: 99%

Raman Assessment of Bone Quality

Morris

Mandair

2011

Clinical Orthopaedics &Amp; Related Research

439

477

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Background Progress in the diagnosis and prediction of fragility fractures depends on improvements to the understating of the compositional contributors of bone quality to mechanical competence. Raman spectroscopy has been used to evaluate alterations to bone composition associated with aging, disease, or injury. Questions/purposes In this survey we will (1) review the use of Raman-based compositional measures of bone quality, including mineral-to-matrix ratio, carbonateto-phosphate ratio, collagen quality, and crystallinity; (2) review literature correlating Raman spectra with biomechanical and other physiochemical measurements and with bone health; and (3) discuss prospects for ex vivo and in vivo human subject measurements. Methods ISI Web of Science was searched for references to bone Raman spectroscopy in peer-reviewed journals. Papers from other topics have been excluded from this review, including those on pharmaceutical topics, dental tissue, tissue engineering, stem cells, and implant integration. Results Raman spectra have been reported for human and animal bone as a function of age, biomechanical status, pathology, and other quality parameters. Current literature supports the use of mineral-to-matrix ratio, carbonate-tophosphate ratio, and mineral crystallinity as measures of bone quality. Discrepancies between reports arise from the use of band intensity ratios rather than true composition ratios, primarily as a result of differing collagen band selections. Conclusions Raman spectroscopy shows promise for evaluating the compositional contributors of bone quality in ex vivo specimens, although further validation is still needed. Methodology for noninvasive in vivo assessments is still under development.

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“…In the mentioned work, BGP was suggested to have a role in the growth of hydroxyapatite crystals via an increase in the degree of carbonate substitutions. 38 Moreover, studies conducted in Ciprinus carpio (carp) rib bone showed a positive correlation between BGP and mineralization, 39 as well as enhanced binding to hydroxyapatite in the presence of calcium. 40 Collectively, these and our observations suggest that BGP is not a passive outcome of the mineralization process, but rather an active contributor, stimulating the differentiation and mineralization of chondrocytes and VSMCs.…”

Section: Idelevich Et Al Bgp Increases Hif-1␣-dependent Metabolism E67mentioning

confidence: 99%