“…To each bottle, one of the following treatments was applied: 1) control (C; no supplemental zinc), 2) 20 ppm of Zn as ZnS, or 3) 20 ppm of Zn as ZnP. Samples were incubated in triplicate and cumulative gas production was monitored at 2, 4,6,8,10,12,15,19,24,30,36,48,72, and 96 h after incubation. Three bottles with incubation medium only were used as blanks to correct the gas production values for gas release from the rumen contents.…”