Effect of opiate receptor blockade by naloxone on development of ischemic cardiac arrhythmias

Kositskii, G. I.; Mikhailova, S. D.; Gorozhanin, S. L.; Semushkina, T. M.

doi:10.1007/bf00841757

Cited by 3 publications

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“…Hemodynamic parameters retuned to normal after 5-10 rain and did not differ from the initial values during reperfusion. In untreated animals these parameters considerably decreased by the 30th second of acute myocardial ischemia [8], hence DYN prevents the decrease in blood pressure at the initial stages of myocardial ischemia, which was demonstrated to represent a good prognostic sign [4]. CO against the background of DYN infusion was accompanied by the appearance of idioventricular rhythm in 42% animals.…”

Section: Resultsmentioning

confidence: 98%

“…Experiments on the isolated heart showed that DYN increased the incidence of cardiac arrhythmia and cAMP concentration in the heart, while naloxone completely abolished these effects [11]. Neurogenie mechanisms play an important role in the pathogenesis of ischemic cardiac arrhythmias [3]. Moreover, n-opiate receptors were found in brain regions involved in the regulation of the cardiovascular system and in sympathetic postganglionic fibers innervating coronary vessels and cardiomyocytes [14].…”

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confidence: 97%

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Role of sympathetic nervous system in protective effects of selectivek-opiate receptor agonist dynorphin A1–13 on the incidence of cardiac arrhythmia during myocardial ischemia

et al. 2000

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Preliminary administration of dynorphin AH3 tO narcotized cats with myocardial ischemia attenuated cardiac fibrillation, but increased the incidence of ventricular tachycardia. Protective effect of dynorphin A1_13 was observed only under conditions of intact sympathetic cardiac innervation. Key Words: dynorphin A~.13; to-opiate receptors; myocardial ischemia; cardiac arrhythmia; stellate ganglionRecent studies demonstrated the presence of la-,~5-, and K:-opiate receptors in the heart. Dynorphin AH3 (DYN) is a selective agonist of n-opiate receptors [6]. Agonists of ~t-and ~-opiate receptors posses considerable antiarrhythmic properties in myocardial ischemia [7,12], but the role of~:-opiate receptors and the effects of DYN on heart rhythm received little attention. Experiments on the isolated heart showed that DYN increased the incidence of cardiac arrhythmia and cAMP concentration in the heart, while naloxone completely abolished these effects [11]. Neurogenie mechanisms play an important role in the pathogenesis of ischemic cardiac arrhythmias [3]. Moreover, n-opiate receptors were found in brain regions involved in the regulation of the cardiovascular system and in sympathetic postganglionic fibers innervating coronary vessels and cardiomyocytes [14]. Here we studied the effects of DYN on the development of cardiac arrhythmias and possible sympathetic mechanisms of this action. MATERIALS AND METHODSExperiments were performed on 49 male and female cats weighing 2-4 kg and narcotized with Nembutal Russian State Medical University, Moscow (40 mg/kg intraperitoneally). Myocardial ischemia was induced by occlusion of circumflex branch of the left coronary artery. The development of arrhythmia was monitored over 15-min myocardial ischemia and 15-min reperfusion. Our previous studies showed that under these conditions, idioventricular rhythm, ventricular tachycardia, and ventricular fibrillation were observed in 72, 28, and 56% observations, respectively [8].Electrocardiogram and blood pressure in the femoral artery and left ventricle were recorded on a Biokomb-8 polyphysiograph (Orion/EMG).The selective ~:-opiate receptor agonist DYN (Laboratory of Peptide Synthesis, Russian Cardiology Research Center) in a dose of 40 pg/kg was intravenously infused for 15 min from the moment of coronary occlusion.In series I (n=12), coronary occlusion (CO) was performed against the background of DYN administration and intact cardiac innervation. In series II (n=10), CO and infusion of DYN was preceded by bilateral vagotomy and transection of cardiac branches of the stellate ganglia (5 min before CO). In series III, cardiac branches of stellate ganglia were transected 5 min before CO in animals with (n=10) or without (n= 10) DYN infusion. In series 4 (n=7), DYN effects on adrenergic properties of the heart were studied by 0007-4888/00/0001-27525.00 9Kluwer Academic/Plenum Publishers

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confidence: 98%

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confidence: 97%

Role of sympathetic nervous system in protective effects of selectivek-opiate receptor agonist dynorphin A1–13 on the incidence of cardiac arrhythmia during myocardial ischemia

et al. 2000

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“…The early stage of myocardial ischemia (30 sec after CO) in this group was characterized by insignificant shifts of hemodynalnic parameters, while in the control group (myocardial ischemia without DAGO) these shifts were more pronounced [5]. Thus, DAGO prevented the ischemia-h~duced drop of hemodynamic parmneters in the early occlusion period, which is regarded as a good prognostic sign [2]. Our experiments showed that injection of the selective Ct-OR agonist DAGO in myocardial ischemia reduces the occurrence of idioventricular arrhythmias and ventricular fibrillation 2-and 5-fold, respectively (/)<0.05) in comparison with the control group (Fig.…”

Section: Resultsmentioning

confidence: 96%

Role of vagus nerves in antiarrhythmic effect of DAGO in acute myocardial ischemia

et al. 1997

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Acute experiments on cats show that DAGO, a selective ~t-opiate receptor agonist, elicits a pronounced antiarrhythmic effect in ischemia-induced arrhythmias. The protective effect of DAGO is observed only under conditions of intact parasympathetic ilmervation of the heart and apparently depends on the n. vagus activity and stimulating effect of DAGO on acetylcholine release. Key Words: DAGO; ~t-opiate receptors; myocardial ischemia; cardiac arrhythmias; vagus nervesAntiarrhythlnic effect of enkephalins in myocardial ischemia is mediated through specific receptors of different types. Since nonselective agonists of opiate l'eceptols (OR) are usually used in experiments, there is no need to identify these receptors. On the other hand, arrhythmogenic effect of enkephalins depends on functional activity of vagus nerves [7]. In light of this, of particular interest is the role of selective agonists of OR in the development of ischemic arrhythlnias. It has been shown that the selective ~t-OR agonist DAGO exhibits antiarrhythmic effect on the development of adrenaline-induced arrhytlunias [3,13]. Moreover, DAGO possesses an analgesic activity, inhibits lipid peroxidation and improves organism's resistance to hypoxia [1,8,9,14]. Taking into account these phannacodynamic effect of DAGO and the fact that antiarrhythmic activity of enkephalins depends on functional activity of the autonomous nerve system, this study was aimed at elucidating the effect of the selective ~t-OR agonist DAGO on the development of arrlwthlnias in myocardial ischemia and the role of the autonomous nerve system in this process. Russian S~aie Mc~tical University, Moscow MATERIALS AND METHODSA total of 57 experiments were performed on cats of both sexes weighing 2-4 kg under Nembutal narcosJ'; (40 mg/kg intraperitoneally). Myocardial ischemia was modeled by occluding the circumflex branch of the left coronary artery near the main vessel. Arrhythmias were recorded during 15-rain occlusion and subsequent 15-min reperfusion periods. We have shown that under these conditions idioventricular arrhythmias develop in 72% of cases, ventricular tachycardia in 28% of cases, and ventricular fibrillation in 55% of cases [5]. Electrocardiogranl and blood pressure in the femoral aftelT were recorded with a Biokomb-8 polyphysiograph (ORION/EMG). DAGO, a selective ~t-OR agonist (synthesized at the Laboratory of Peptide Synthesis, Russian Cardiology Research Center) were infnsed over the occlusion period in a dose of 20 txg/kg. The following interventions were performed: group 1 (n=ll) --coronary occlusion (CO) and injection of DAGO under conditions of intact innervation; group 2 (n=8) --CO and injection of DAGO under conditions of bilateral transection of the vagus nerve on the neck and sym-

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“…Key Words: laser radiation; ischemic cardiac arrhythmias; stimulation of sensorimotor cortex; opioid peptides; vagus nerve Animals with experimental myocardial ischemia (MI) complicated by ventricular fibrillation show disintegrated neuronal activity in the bulbar cardiovascular center mainly because of increased flow of afferent information from the ischemic myocardium via the vagus nerves to the bulbar centers and cortical structures involved in the regulation of the cardiovascular system activity. Stimulation of these structures during MI considerably increases the occurrence of all ventricular arrhythmias [3]. The fact that cortical centers play an important role in the development of ischemic arrhythmias is confirmed by the high occurrence of severe arrhythmias in MI patients with emotional and other types of pathological stress [9,11].…”

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The mechanism of action of laser radiation on the development of ischemic arrhythmias after stimulation of the sensorimotor cortex

et al. 1996

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Acute experiments on Nembutal-anesthetized cats with occluded circumflex branch of the left coronary artery reveal an antiarrhythmic effect of laser radiation even against the background of the sensorimotor cortex stimulation. This effect may result from the correcting influence of intra-atrial laser irradiation on the activity of bulbar cardiovascular center through the afferent fibers of the vagus nerve and the opioid peptide system. Key Words: laser radiation; ischemic cardiac arrhythmias; stimulation of sensorimotor cortex; opioid peptides; vagus nerve Animals with experimental myocardial ischemia (MI) complicated by ventricular fibrillation show disintegrated neuronal activity in the bulbar cardiovascular center mainly because of increased flow of afferent information from the ischemic myocardium via the vagus nerves to the bulbar centers and cortical structures involved in the regulation of the cardiovascular system activity. Stimulation of these structures during MI considerably increases the occurrence of all ventricular arrhythmias [3]. The fact that cortical centers play an important role in the development of ischemic arrhythmias is confirmed by the high occurrence of severe arrhythmias in MI patients with emotional and other types of pathological stress [9,11].Bearing in mind the effectiveness of laser therapy in the treatment of ischemic heart disease, we attempted to find out whether the antiarrhythmic effect of laser radiation is altered in animals with acute MI following stimulation of the sensorimotor cortex zone participating in the development of stress reactions. Russian State Medical University, Moscow MATERIALS AND METHODSThe study was conducted on Nembutal-anesthetized (40 mg/kg intraperitoneally), artificially ventilated adult cats of both sexes weighing 2-4 kg. After thoracotomy, the pericardium was excised, the circumflex branch of the left coronary artery was dissected near its orifice, and a dederone thread was passed to that site. Myocardial ischemia was produced by clamping the left coronary artery for 15 min and followed by reperfusion. In all cats, electrostimulation of the sensorimotor cortex was started 10 sec prior to clamping the coronary artery and lasted 20 see. Monopolar silver electrodes (tip diameter 0.8 ram) were inserted in the sigmoid sulcus after craniotomy and dissection of the dura mater. Stimulation was performed with rectangular pulses (1 msec, 30 Hz) from an ESL-1 apparatus. Pulse intensity ranged from 2.5 to 6.5 mA depending on the magnitude of the resistive voltage drop measured with an S1-48B oscillograph. The precise position of the stimulating electrode was ascertained after extensive craniotomy.

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Effect of opiate receptor blockade by naloxone on development of ischemic cardiac arrhythmias

Cited by 3 publications

References 3 publications

Role of sympathetic nervous system in protective effects of selectivek-opiate receptor agonist dynorphin A1–13 on the incidence of cardiac arrhythmia during myocardial ischemia

Role of sympathetic nervous system in protective effects of selectivek-opiate receptor agonist dynorphin A1–13 on the incidence of cardiac arrhythmia during myocardial ischemia

Role of vagus nerves in antiarrhythmic effect of DAGO in acute myocardial ischemia

The mechanism of action of laser radiation on the development of ischemic arrhythmias after stimulation of the sensorimotor cortex

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