Effect of oocyte quality on blastocyst development after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in a sheep model

Catalá, M. G.; Izquierdo, Dolors; Rodríguez-Prado, M.; Hammami, Sondes; Paramio, M. T.

doi:10.1016/j.fertnstert.2011.12.043

Cited by 51 publications

(52 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…of patients, the correlation between AOPP concentration in the FF and blastocyst rate presented a significant negative correlation. The low success rate of ART may be explained by the low oocyte quality and blockage of embryo development (63). In clinical practice, selecting good-quality oocytes and embryos is key for promoting ART.…”

Section: Discussionmentioning

confidence: 99%

Advanced oxidation protein products from the follicular microenvironment and their role in infertile women with endometriosis

et al. 2017

View full text Add to dashboard Cite

Abstract. Endometriosis (EM) is associated with oxidative stress. Advanced oxidation protein products (AOPPs) are novel markers of oxidative stress, which serve an important role as an inflammatory mediator in various chronic diseases. In order to examine the role of AOPPs in infertile women with EM, the present study analyzed the levels of AOPPs, estradiol (E 2 ) and progesterone (P 4 ) in the follicular fluid (FF) of 89 women with or without EM undergoing in vitro fertilization (IVF). The AOPP concentration in the FF of the EM group was significantly higher when compared with that of the control group (51.5±22.4 vs. 41.8±18.3 µmol/l; P<0.05). However, the FF P 4 levels and blastocyst rate were significantly lower in the EM group compared with the control group (P 4 :1,249.6±465.4 vs. 1,752.7±565.4 ng/ml, P<0.05; blastocyst rate: 0.511±0.322 vs. 0.662±0.278; P<0.05). The AOPP concentration and P 4 level in the FF presented a significant negative correlation in the EM and control groups, as well as in the total cohort of patients (EM group: r=-0.406, P=0.006; control group: r=-0.315, P=0.035; total: r=-0.421, P<0.001). In addition, there was a significant negative correlation between the FF AOPP concentrations and blastocyst rate in the EM group and in the total cohort (EM group: r=-0.376, P=0.012; total: r=-0.367, P<0.001). In conclusion, these results suggested that AOPPs may be a potentially effective marker for predicting the oocyte quality and outcomes of IVF in infertile women with EM.

show abstract

Section: Discussionmentioning

confidence: 99%

Advanced oxidation protein products from the follicular microenvironment and their role in infertile women with endometriosis

et al. 2017

View full text Add to dashboard Cite

show abstract

“…We believe that in vitro matured oocytes shall give us great and easily repeatable results in every laboratory of ruminant reproduction. The low success of ICSI in sheep has generally been ascribed to inadequate oocyte activation by ram spermatozoon; therefore, an external stimulus has usually been provided [3,4,11,30].…”

Section: Discussionmentioning

confidence: 99%

Plasma membrane and acrosome loss before ICSI is required for sheep embryonic development

Anzalone

Iuso

Czernik

et al. 2016

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose This study aims to determine if the integrity of the sperm plasma membrane and acrosome vesicle could be limiting factors in sheep intracytoplasmic sperm injection (ICSI). Methods Prior to in vitro fertilization (IVF) or ICSI, the oocytes were subjected to in vitro maturation (IVM) for 24 h. First, to evaluate the need of artificial activation for ovine ICSI, 226 oocytes were injected with intact spermatozoa (IS), from which 125 were activated by incubation in ionomycin and 101 were cultured without activation. Next, spermatozoa were mechanically (by piezo-electrical pulses) and/or chemically (by ionomycin/Triton X-100) treated to break membranes and acrosomes and were injected into oocytes, grouped as follows: (i) piezo-pulsed spermatozoa (PPS), (ii) PPS pre-treated with ionomycin (PPS-I), (iii) PPS pre-treated with Triton X-100 (PPS-T), and (iv) intact and untreated spermatozoa as a control (CTR-IS). Results No differences were observed in the zygote/cleavage/ blastocyst rate between chemically activated and nonactivated oocytes (50 vs. 45 %, 11.6 vs. 10.1 %; 1.8 vs. 1.1 %, respectively), after ICSI with CTR-IS. Injection of PPS compared to CTR-IS increased the proportion of zygotes and blastocysts (84.6 vs. 45 %, p < 0.01; 15.5 vs. 1.1 %, p < 0.0001, respectively). Moreover, the percentage of PPSderived blastocysts was not significantly different from that obtained by conventional IVF (15.5 vs. 20.2 %). The ICSI blastocysts' development was also improved with PPS pretreated with ionomycin (15.6 %), but was completely impeded with PPS pre-treated with Triton X-100 (0 %). Conclusion Our findings confirm that ICSI with spermatozoa whose plasma membrane and acrosome have been mechanically damaged substantially improves embryonic development until the blastocyst stage.

show abstract

“…In this assay, the activity of glucose-6-phosphate dehydrogenase (G6PDH) is measured: oocytes with blue colour are regarded to be fully developmentally competent, while colourless gametes are regarded as incompetent (Roca et al 1998;Kempisty et al 2011). There are numerous studies using both BCB positive (BCB + ) and BCB negative (BCB -) oocytes (Wongsrikeao et al 2006;Catala et al 2012). Therefore, this study was aimed at investigating the key regulators of cell cycle division-cyclin-dependent kinase inhibitors (Cdkns) in developmentally competent and incompetent porcine oocytes.…”

Section: Discussionmentioning

confidence: 99%

Expression of cyclin-dependent kinase inhibitors (CDKN1, CDKN5) in developmentally competent and incompetent porcine oocytes

Piotrowska¹,

Kempisty²,

Bukowska³

et al. 2013

Vet. Med.

View full text Add to dashboard Cite

Although several reports have been published regarding the cyclin-dependent kinases (Cdks) in developmentally competent mammalian oocytes, still little is known about their expression pattern in developmentally incompetent female gametes. Since Cdks are the main cell cycle division regulators, also described as "checkpoints" in the MI to MII transition, the aim of this study was to investigate the differential mRNA expression of genes encoding Cdkn1 and Cdkn5 in developmentally competent and incompetent porcine oocytes. Porcine cumulus-oocyte complexes (COCs) were collected from crossbred Landrace gilts after slaughter and partly subjected to brilliant cresyl blue (BCB) staining and in vitro maturation (IVM). Three groups of COCs were analysed: (i) oocytes soon after collection without BCB staining, (ii) oocytes which remained colourless after staining (BCB -) and (iii) COCs which stained blue (BCB + ). BCB + COCs were additionally cultured in standard porcine medium (TCM199) for 44 h. All oocytes were analysed using QT-PCR targeting CDKN1 and CDKN5 mRNA expression. The highest CDKN1 mRNA expression was found in oocytes without BCB staining and IVM (Group I) compared to BCB -and BCB + oocytes, (P < 0.001, P < 0.01, respectively). An increased CDKN5 mRNA level was observed in BCB + oocytes (Group III) compared to oocytes of Group I and Group II (P < 0.01), respectively. The mRNA expression of both genes was always higher in BCB + compared to BCB -oocytes. Based on this study it can be supposed that CDKN1 and CDKN5 are differentially expressed in developmentally distinct porcine oocytes and in a maturation stage-dependent manner. Moreover, CDKN1 may play a role as a molecule which regulates cell cycle arrest and the specific "block of maturation" from MI to MII.

show abstract

Effect of oocyte quality on blastocyst development after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in a sheep model

Cited by 51 publications

References 19 publications

Advanced oxidation protein products from the follicular microenvironment and their role in infertile women with endometriosis

Advanced oxidation protein products from the follicular microenvironment and their role in infertile women with endometriosis

Plasma membrane and acrosome loss before ICSI is required for sheep embryonic development

Expression of cyclin-dependent kinase inhibitors (CDKN1, CDKN5) in developmentally competent and incompetent porcine oocytes

Contact Info

Product

Resources

About