Effect of Omega-3 Fatty Acids on Erythrocyte Membrane in Diabetic Rats

Hussein, Jihan; Mostafa, Eman; El-Waseef, Maha; El-Khayat, Zakaria; Badawy, Ehsan; Medhat, Dalia

doi:10.3889/mjms.1857-5773.2011.0180

Cited by 11 publications

(4 citation statements)

References 33 publications

(29 reference statements)

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“…MDAs were significantly increased in the RBC membranes of diabetic rats, which is consistent with the results of previous studies (28,29). In contrast, SOD activity in RBC membranes was higher in diabetic rats than in control rats (30), which could be due to the activation of an antioxidant defense system to suppress the formation of free radicals (25).…”

Section: Discussionsupporting

confidence: 93%

The protective effect of aqueous extracts of roselle (Hibiscus sabdariffa L. UKMR-2) against red blood cell membrane oxidative stress in rats with streptozotocin-induced diabetes

Mohamed¹,

Shing²,

Idris³

et al. 2013

Clinics

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OBJECTIVES:The aim of this study was to investigate the protective effects of aqueous extracts of roselle (Hibiscus sabdariffa L. UKMR-2) against red blood cell (RBC) membrane oxidative stress in rats with streptozotocin-induced diabetes.METHODS:Forty male Sprague-Dawley rats weighing 230-250 g were randomly divided into four groups (n = 10 rats each): control group (N), roselle-treated control group, diabetic group, and roselle-treated diabetic group. Roselle was administered by force-feeding with aqueous extracts of roselle (100 mg/kg body weight) for 28 days.RESULTS:The results demonstrated that the malondialdehyde levels of the red blood cell membranes in the diabetic group were significantly higher than the levels in the roselle-treated control and roselle-treated diabetic groups. The protein carbonyl level was significantly higher in the roselle-treated diabetic group than in the roselle-treated control group but lower than that in the diabetic group. A significant increase in the red blood cell membrane superoxide dismutase enzyme was found in roselle-treated diabetic rats compared with roselle-treated control rats and diabetic rats. The total protein level of the red blood cell membrane, osmotic fragility, and red blood cell morphology were maintained.CONCLUSION:The present study demonstrates that aqueous extracts of roselle possess a protective effect against red blood cell membrane oxidative stress in rats with streptozotocin-induced diabetes. These data suggest that roselle can be used as a natural antioxidative supplement in the prevention of oxidative damage in diabetic patients.

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Section: Discussionsupporting

confidence: 93%

The protective effect of aqueous extracts of roselle (Hibiscus sabdariffa L. UKMR-2) against red blood cell membrane oxidative stress in rats with streptozotocin-induced diabetes

Mohamed¹,

Shing²,

Idris³

et al. 2013

Clinics

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“…After 3 days’ acclimatisation, the rats commenced consumption of a high-fat diet (HFD), which continued for 5 weeks and was followed by two intraperitoneal injections of low-dose streptozotocin (STZ; 30 mg/kg), 1 week apart [ 21 ]. STZ was dissolved in 50 mM sodium citrate solution (pH 4.5) containing 150 mM NaCl [ 22 ]. After the induction of diabetes using HFD and STZ, fasting blood glucose (FBG) levels were measured in the blood from the tail vein using a glucometer.…”

Section: Methodsmentioning

confidence: 99%

The Effect of Puguntano Leaf Extract (Curanga Fel - Terrae Merr.) On P38 Mapk Levels and Glut-4 Expression in Type 2 Diabetic Rat Muscle

Syafril¹,

Lindarto²,

Lelo³

et al. 2019

Open Access Maced J Med Sci

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BACKGROUND: Puguntano (Curanga feel-terrae Merr.) contains flavonoids, saponins, tannins, and steroids/ terpenoids which improved post-receptor insulin signalling in rats model of type 2 diabetes mellitus (T2DM). AIM: This study aimed to determine the effect of puguntano leaf extract on p38 mitogen-activated protein kinase (MAPK) levels and glucose transporter-4 (GLUT-4) expression in diabetic rats muscle. METHODS: Forty-eight male Wistar rats had T2DM induced using a combination of feeding a high-fat diet for 5 weeks and multiple intraperitoneal injections of low-dose streptozotocin (30 mg/kg). The diabetic rats were randomly divided into control and treatment groups, and 200 mg/kg/day puguntano extract was administered orally for 10 days to treatment group. Subsequently, p38 MAPK levels were measured by Sandwich Elisa and plasma membrane GLUT-4 expression was evaluated by Immunohistochemistry in their gastrocnemius muscles. RESULTS: There were significantly higher p38 MAPK levels and GLUT-4 expression in the treatment group than in the control group. CONCLUSION: These data suggest that a puguntano leaf extract can improve post-receptor insulin signalling by enhancing p38 MAPK levels and GLUT-4 expression in a rat model of T2DM.

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“…Giron dkk, 20 dalam penelitiannya pada tikus diabetes, menunjukkan bahwa konsumsi Omega-3 menurunkan kadar gula darah dengan meningkatkan transporter Glut-4 yang akan menginduksi transport glukosa ke otot. Hussein dkk, 21 dalam penelitiannya di Mecedonia, juga mendapatkan penurunan kadar glukosa puasa setelah pemberian Omega-3 pada tikus yang dibuat menderita diabetes mellitus. Penelitian ini tidak membandingkan kadar glukosa puasa sebelum dan setelah pemberian Omega-3.…”

Section: Pembahasanunclassified

“…Hal tersebut disebabkan oleh pengaruh Omega-3 yang dapat meningkatkan sensitifitas terhadap insulin sehingga menurunkan kadar gula darah. 3,21 Lopez dkk, 22 dalam penelitiannya pada 201 anak obes, mendapatkan bahwa pemberian Omega-3 selama 12 minggu dapat menurunkan resistensi insulin dan kadar trigliserida.…”

Section: Pembahasanunclassified

Pengaruh Pemberian Omega-3 Terhadap Kadar C-Reactive Protein pada Remaja Obes Resistensi Insulin

Wilson

Rini

Bachtiar

2016

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Latar belakang. Obesitas berperan penting terhadap terjadinya sindrom metabolik yang dapat menyebabkan resistensi insulin,dislipidemia, diabetes mellitus dan hipertensi yang akan meningkatkan risiko penyakit jantung koroner. Jaringan adiposa padaobesitas akan memproduksi berbagai sitokin antara lain TNF-α dan IL-6 yang selanjutnya merangsang hati untuk menghasilkanC-RP. Omega-3 mempunyai efek anti inflamasi, dapat meningkatkan sensitifitas insulin, mengurangi resistensi insulin, perlemakanhati dan memperbaiki profil lemak serta menurunkan kadar C-RP.Tujuan. Mengetahui pengaruh pemberian Omega-3 terhadap kadar C-RP pada remaja obesitas resistensi insulin.Metode. Penelitian eksperimental dari November 2011 sampai Maret 2012 dengan disain pre and post-test group. Obesitas ditentukanberdasarkan pengukuran indek massa tubuh (IMT) >p-95 WHO-NCHS, resistensi insulin menurut kadar homeostasis model assesmentinsulinresistence (HOMA-IR) >3,16. Sampel terpilih diperiksa kadar C-RP sebelum dan sesudah pemberian Omega-3. Omega-3diberikan selama 3 bulan dengan dosis 250 mg perhari. C-RP diperiksa secara Elisa dengan metode CMIA (chemiluminescentImmunometriz assay), sensitivitas 0,01 mg/dL. Data diolah menggunakan paired t-test (α=0,05).Hasil. Didapatkan 75 orang remaja obesitas dan 21 orang di antaranya (50,7%) mengalami resistensi insulin. Sebanyak 21 sampeldiuji, lebih dari separuh laki-laki (57,1% vs 42,9%). Rerata umur 16±0,68 tahun, rerata IMT 32,88±2,44 kg/m2. Rerata kadarglukosa, insulin puasa dan HOMA-IR, masing-masing adalah 90,36±14,94 mg/dl, 20,89±4,19 μIU/L dan 4,52±0,89. Terdapatpenurunan kadar C-RP sebelum dan sesudah pemberian Omega-3, yaitu 8,69±5,46 mg/dL menjadi 6,41±4,77 mg/dL. Penurunanini bermakna secara statistik (p=0,018).Kesimpulan. Konsumsi Omega-3 selama 3 bulan menurunkan kadar C-RP remaja obesitas resistensi insulin.

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Effect of Omega-3 Fatty Acids on Erythrocyte Membrane in Diabetic Rats

Abstract: Background: Diabetes mellitus is a metabolic disease characterized by chronic hyperglycemia resulting from defects in insulin secretion, almost always with a major contribution from insulin resistance which may be affected by cell membrane fatty acids and phospholipids fractions.

Cited by 11 publications

References 33 publications

The protective effect of aqueous extracts of roselle (Hibiscus sabdariffa L. UKMR-2) against red blood cell membrane oxidative stress in rats with streptozotocin-induced diabetes

The protective effect of aqueous extracts of roselle (Hibiscus sabdariffa L. UKMR-2) against red blood cell membrane oxidative stress in rats with streptozotocin-induced diabetes

The Effect of Puguntano Leaf Extract (Curanga Fel - Terrae Merr.) On P38 Mapk Levels and Glut-4 Expression in Type 2 Diabetic Rat Muscle

Pengaruh Pemberian Omega-3 Terhadap Kadar C-Reactive Protein pada Remaja Obes Resistensi Insulin

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