Effect of Ocimum sanctum on Oral Cancer Cell Line: An in vitro Study

Shivpuje, Prachi; Ammanangi, Renuka; Bhat, Kishore; Katti, Sandeep

doi:10.5005/jp-journals-10024-1745

Cited by 18 publications

(6 citation statements)

References 9 publications

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“…In addition, fluorescent staining with Hoechst 33258 showed increased and consistent nuclear condensation and shrinkage, cell growth inhibition and apoptotic body formation in EO treated colon cancer cells in comparison to control cells that showed rounded intact nuclei. Similar findings on the Ocimum viride essential oil were also reported by Sharma and coworkers against COLO 205 cells (Sharma et al, 2016), other species from Ocimum genus such as Ocimum basilium showed in vitro cytotoxicity against cancer cells (Zarlaha et al, 2014), Ocimum sanctum showed to trigger cell death by apoptosis against human breast, liver and oral cancer cells etc (Manaharan et al, 2016;Sharma et al, 2016;Shivpuje et al, 2015). Molecular docking analysis was performed to confirm the binding efficacy of thymol from essential oil to Beclin protein that functions in autophagy, it interacts with class III PI3K complex components and Bcl-2 and lysosomal degradation pathway (Domínguez-Martín et al, 2018;Kim et al, 2017;Li et al, 2017).…”

Section: Discussionsupporting

confidence: 71%

Chemical, biological and in silico assessment of Ocimum viride essential oil

Bhagat

Sangral

Kumar

et al. 2020

Heliyon

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Ocimum viride Willd. (family: Lamiaceae) is a member of the genus Ocimum, an aromatic annual and perennial herb with numerous culinary, horticultural and ethno-medicinal benefits. This study aims to explore the chemical properties of leaf essential oil (EO) from Ocimum viride and to evaluate its antimicrobial and anticancer potential. Main methods: Characterization of essential oil was done by GCMS, antimicrobial by agar well diffusion methods, in vitro cytotoxicity evaluation by MTT assay, cell death analysis was done by DNA fragmentation, cell cycle analysis, nuclear morphology analysis and molecular docking studies were also conducted. Key findings: Essential oil from aerial parts (leaf) of Ocimum viride revealed high content of oxygenated monoterpenes, notably thymol (~50%) and γ-terpinene (~18%). Further, antibacterial analysis showed that among all the evaluated bacterial species EO showed highest sensitivity against the Bacillus subtilis and was also found most effective against HT-29 colon cancer cell line with IC50 value of~0.034 AE 0.001μL/mL. Mechanistic studies revealed that EO inhibits the growth of HT-29 colon cancer cells probably through induction of irreparable DNA damage leading to subsequent cell death in apoptotic manner. Molecular docking analysis also supports the in vitro studies conducted by indicating the interaction of thymol with Sec A protein of Bacillus subtilis cell wall as well as with Beclin protein responsible for apoptotic corpse clearance. Significance: Taken together, our results indicate that EO possesses potent antimicrobial and anticancer properties, and may find applications as effective antibacterial and in cancer therapeutics.

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Section: Discussionsupporting

confidence: 71%

Chemical, biological and in silico assessment of Ocimum viride essential oil

Bhagat

Sangral

Kumar

et al. 2020

Heliyon

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“…13 Correspondingly in our study canagliflozin and dapagliflozin also have shown significant loss of cell viability in oral squamous cell carcinoma KB cell lines at concentrations of 250µg/ml and 400µg/ml in comparison with standard drug 5-fluorouracil, also we have detected the cells underwent reduction and decline in number exhibiting positive cytotoxic activity and the probable mechanism would be because of the restriction of glucose uptake and curbing Mitochondrial intracellular ATP synthesis thereby limiting the cell cycle of cancer cells. 21 In present study canagliflozin was more cytotoxic than dapagliflozin in KB cell line as established by IC50 values.…”

Section: Discussionmentioning

confidence: 49%

Evaluation and comparison of anti-cancer activity of dapagliflozin and canagliflozin in oral cancer cell line: an in vitro study

Vinay

David

2019

Int J Basic Clin Pharmacol

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Background: Cancer is rapidly evolving life-threatening ailment in the mankind due to changes in daily food intake and lifestyle changes. Oral carcinoma is 6th major cause of cancer death in the world and it is third major reason of cancer mortality in India. Every cell in the human body requires glucose for its metabolic energy. Besides normal cell, cancer cells also require the glucose for its endurance and multiplication. SGLT2 inhibitors which are aimed at diabetes therapy exhibited anticancer properties also in colon and pancreatic cancer lines. Present study aim is to evaluate the anticancer activity of SGLT2 inhibitors against oral cancer cell by MTT Assay.Methods: To evaluate the anticancer activity of SGLT2 inhibitors MTT Cytotoxic assay is performed as per standard protocols. Cancer cells were plated in 24-well plates and incubated at 370C with 5% CO2 condition. After convergence, samples are added to the plates in various concentrations and allowed to incubate then they are detached from the plates and cleansed with the reagents. The wells are coated with the dye and incubated. Later samples are analysed in UV-spectrophotometer.Results: Cytotoxic assay showed decrease in cell viability with increasing dose of SGLT2 inhibitors. IC50 values were determined graphically. The IC50 value of dapagliflozin is 400µg/ml and canagliflozin is 250µg/ml respectively after 24 hours of Assessment.Conclusions: The results of the current study give us an evidence that SGLT2 inhibitors dapagliflozin and canagliflozin exhibits anticancer property in Oral Cancer cell line.

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“…The induction of intrinsic and extrinsic apoptotic pathways by B. serrata extract and boswellic acids has also been demonstrated (29). Numerous plant extracts have been shown to have the ability to stop cancer cells from proliferating, making them useful in the treatment of cancer (27,30). According to Huang et al, a B. serrata extract containing β boswellic acids and related compounds prevents mice from developing tumours when induces tumour growth by preventing the up-regulation of pro-inflammatory cytokine proteins.…”

Section: Resultsmentioning

confidence: 99%

EVALUATION OF ANTI TUMORIGENIC ACTIVITY OF Boswelliaserrata USING KB CELL LINE

Michael¹,

Devi.R²,

Selvaraj³

2022

Journal of Pharmaceutical Negative Results

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Background: B.serrata is commonly known as Indian olibanum, it is mainly used in the production of Indian frankincense. B.serrata is used for many other purposes including asthma diabetes. The medicinal plant Boswelliaserrata has great potential for treating cancer. Since many cancer therapies have their origins in natural ingredients, this study aims to explore the anti-tumorigenic effect of B.serrata using KB cell line.Materials and methods: Seed of B.serrata was collected and extraction was done. Then the extract was added to the culture and the oral cancer cells were obtained from Pune. The cultured oral cells were incubated in a CO2 incubator. Treated oral cancer cell sample was done MTT assay and gene expression analysis.Result: The collected data from MTT assay and gene expression analysis tabulated using SPSS software. MTT assay results showed gradual decrease in cell viability with increase in concentration of Boswelliaserrata and the gene expression showed up regulation of caspase 3 and 9 expression.Conclusion: From the study, Boswelliaserrata has a significant effect on anti-tumorigenic activity; this might be due to the active bio components present in Boswelliaserrata extract.

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Effect of Ocimum sanctum on Oral Cancer Cell Line: An in vitro Study

Cited by 18 publications

References 9 publications

Chemical, biological and in silico assessment of Ocimum viride essential oil

Chemical, biological and in silico assessment of Ocimum viride essential oil

Evaluation and comparison of anti-cancer activity of dapagliflozin and canagliflozin in oral cancer cell line: an in vitro study

EVALUATION OF ANTI TUMORIGENIC ACTIVITY OF Boswelliaserrata USING KB CELL LINE

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